Special feature: Method of the Year 2014

Method of the Year 2014 - p1

doi:10.1038/nmeth.3251

Light-sheet fluorescence microscopy can image living samples in three dimensions with relatively low phototoxicity and at high speed.

Abstract - Method of the Year 2014 | Full Text - Method of the Year 2014 | PDF (65 KB) - Method of the Year 2014

Special feature: Method of the Year 2014

Pump up the volume - pp19 - 22

Michael Eisenstein

doi:10.1038/nmeth.3220

Light-sheet fluorescence microscopy techniques are enabling researchers to achieve dynamic, long-term imaging and three-dimensional reconstruction of specimens ranging from single cells to whole embryos.

Abstract - Pump up the volume | Full Text - Pump up the volume | PDF (522 KB) - Pump up the volume

Special feature: Method of the Year 2014

Light-sheet fluorescence microscopy for quantitative biology - pp23 - 26

Ernst H K Stelzer

doi:10.1038/nmeth.3219

In light sheet–based fluorescence microscopy (LSFM), optical sectioning in the excitation process minimizes fluorophore bleaching and phototoxic effects. Because biological specimens survive long-term three-dimensional imaging at high spatiotemporal resolution, LSFM has become the tool of choice in developmental biology.

Abstract - Light-sheet fluorescence microscopy for quantitative biology | Full Text - Light-sheet fluorescence microscopy for quantitative biology | PDF (438 KB) - Light-sheet fluorescence microscopy for quantitative biology

Special feature: Method of the Year 2014

Light-sheet imaging for systems neuroscience - pp27 - 29

Philipp J Keller, Misha B Ahrens & Jeremy Freeman

doi:10.1038/nmeth.3214

Developments in electrical and optical recording technology are scaling up the size of neuronal populations that can be monitored simultaneously. Light-sheet imaging is rapidly gaining traction as a method for optically interrogating activity in large networks and presents both opportunities and challenges for understanding circuit function.

Abstract - Light-sheet imaging for systems neuroscience | Full Text - Light-sheet imaging for systems neuroscience | PDF (482 KB) - Light-sheet imaging for systems neuroscience

Special feature: Method of the Year 2014

Guide to light-sheet microscopy for adventurous biologists - pp30 - 34

Emmanuel G Reynaud, Jan Peychl, Jan Huisken & Pavel Tomancak

doi:10.1038/nmeth.3222

Ten years of development in light-sheet microscopy have led to spectacular demonstrations of its capabilities. The technology is ready to assist biologists in tackling scientific problems, but are biologists ready for it? Here we discuss the interdisciplinary challenges light-sheet microscopy presents for biologists and highlight available resources.

Abstract - Guide to light-sheet microscopy for adventurous biologists | Full Text - Guide to light-sheet microscopy for adventurous biologists | PDF (840 KB) - Guide to light-sheet microscopy for adventurous biologists

Special feature: Method of the Year 2014

DIA mass spectrometry - p35

Allison Doerr

doi:10.1038/nmeth.3234

Data-independent acquisition (DIA) mass spectrometry may change how proteomic data are generated.

Abstract - DIA mass spectrometry | Full Text - DIA mass spectrometry | PDF (662 KB) - DIA mass spectrometry

Special feature: Method of the Year 2014

Understanding noncoding RNAs - p35

Nicole Rusk

doi:10.1038/nmeth.3235

Methods to profile and characterize the function of noncoding RNAs will emerge.

Abstract - Understanding noncoding RNAs | Full Text - Understanding noncoding RNAs | PDF (662 KB) - Understanding noncoding RNAs

Special feature: Method of the Year 2014

In vivo voltage sensors - p36

Nina Vogt

doi:10.1038/nmeth.3236

Genetically encoded voltage indicators are on the brink of allowing neuronal activity to be directly imaged in vivo.

Abstract - In vivo voltage sensors | Full Text - In vivo voltage sensors | PDF (1,248 KB) - In vivo voltage sensors

Special feature: Method of the Year 2014

Next-generation CRISPRs - p36

Nicole Rusk

doi:10.1038/nmeth.3237

As the CRISPR-Cas system matures, specificity, efficacy and maybe even a eukaryotic nuclease are being considered.

Abstract - Next-generation CRISPRs | Full Text - Next-generation CRISPRs | PDF (1,248 KB) - Next-generation CRISPRs

Special feature: Method of the Year 2014

Structures from tiny crystals - p37

Allison Doerr

doi:10.1038/nmeth.3238

Protein structures can be determined from microcrystals using X-ray and electron diffraction.

Abstract - Structures from tiny crystals | Full Text - Structures from tiny crystals | PDF (535 KB) - Structures from tiny crystals

Special feature: Method of the Year 2014

Super-resolution CLEM - p37

Natalie de Souza

doi:10.1038/nmeth.3239

Correlated light and electron microscopy (CLEM) is particularly powerful when applied in super-resolution.

Abstract - Super-resolution CLEM | Full Text - Super-resolution CLEM | PDF (535 KB) - Super-resolution CLEM

Special feature: Method of the Year 2014

Nanopores for proteins - p38

Tal Nawy

doi:10.1038/nmeth.3240

Nanopores hold promise for single-protein characterization.

Abstract - Nanopores for proteins | Full Text - Nanopores for proteins | PDF (550 KB) - Nanopores for proteins

Special feature: Method of the Year 2014

Imaging at depth - p38

Nina Vogt

doi:10.1038/nmeth.3241

A closer look into the depths of organs such as the brain is within reach.

Abstract - Imaging at depth | Full Text - Imaging at depth | PDF (550 KB) - Imaging at depth