Research

This list contains the 50 most recently published research articles, including advance online publication articles that have not yet been published in a journal issue.

Showing: 1–25 of 50

AOP
  1. Allele-specific expression reveals interactions between genetic variation and environment AOP

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    The EAGLE algorithm and software identifies replicable gene-by-environment interactions based on associations between environment and allele-specific expression.

  2. Nm-seq maps 2′-O-methylation sites in human mRNA with base precision AOP

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    Iterative oxidation, elimination and dephosphorylation steps remove nucleotides from the 3′ end of RNA until a 2′-O-methylated ribose that cannot be oxidized is encountered and brings the process to a stop. High-throughput sequencing of these fragments exposes 2′-O-methyl sites at base resolution.

  3. Generation of pure GABAergic neurons by transcription factor programming AOP

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    Transient transcription factor expression rapidly induces a homogenous population of mature GABAergic neurons from human pluripotent stem cells, aiding the study of inhibitory neuron function and disease.

  4. Mapping the genomic landscape of CRISPR–Cas9 cleavage AOP

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    SITE-Seq probes Cas9 cleavage sites in vitro and returns a comprehensive list of off-target sites at different Cas9–sgRNA concentrations.

  5. Marker-free coselection for CRISPR-driven genome editing in human cells AOP

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    A gain-of-function mutation in a sodium/potassium pump renders cells resistant to a small-molecule drug and provides an efficient coselection strategy to enrich for CRISPR-induced mutations at an independent locus.

  6. A tiling-deletion-based genetic screen for cis-regulatory element identification in mammalian cells AOP

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    CREST-seq allows functional screening of cis-regulatory elements through the use of sgRNA pairs to introduce tiled deletions in regions of interest. The analysis of a 2-megabase target region shows that promoters can act as distal enhancers for unrelated genes.

  7. Iterative expansion microscopy AOP

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    Iterative expansion microscopy (iExM) is a strategy that achieves high resolution expansion microscopy by expanding samples multiple times. Expanding a sample twice enables ∼4.5 × 4.5 ∼20× physical expansion and ∼25 nm resolution.

  8. Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1

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    This paper describes a fully defined, nonxenogeneic in vitro niche for the differentiation of haematopoietic stem and progenitor cells to progenitor T cells in mouse and human.

    See also: News and Views by Anne-Catherine Dolens et al.

  9. DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells

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    DeActs are genetically encoded tools that perturb the actin cytoskeleton. In contrast to drugs such as latrunculin, they can be targeted to specific cell types, which is demonstrated in the developing mouse brain and in Caenorhabditis elegans.

  10. Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoids

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    This paper describes an in vitro method to generate human T cells from hematopoietic stem and progenitor cells (HSPCs). It should be useful for both basic and applied studies using T cells.

    See also: News and Views by Anne-Catherine Dolens et al.

  11. Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain

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    iTango confers access to neuromodulation-sensitive subsets of neurons in a functionally defined and temporally controlled manner. The tool allows for manipulating the subset of neurons that are activated by dopamine during a behavior of interest.