Focuses and supplements


Each year, Nature Methods publishes special collections of articles focused on topics of particular methodological interest. This page provides an index of the supplements, focuses and special features—such as the popular Method of the Year—that we have published over the years.


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2017

Focus on deep imaging of live tissue

Focus on deep imaging of live tissue

One of the goals of biological imaging is to watch biological processes where they occur – within living tissue or even within a living animal. But in vivo imaging presents a set of challenges that are not encountered when imaging relatively small, flat samples like cells. In this Focus issue, we bring together papers on methods for optical imaging within living tissue. Two Reviews discuss: microscopy methods for functional brain imaging and the principles and practicalities of the different flavours of light sheet microscopy. A Perspective describes the use of adaptive optics to correct aberrations in scattering samples. This issue also includes primary research papers on multiphoton microscopy, an editorial that reminds our readers of the many areas of this large and exciting field that we do not cover, and a collection of recent papers on deep imaging from Nature Research journals.

April Vol. 14, No. 4

Method of the Year 2016

Special Feature — Method of the Year 2016

Epitranscriptome analysis is our choice for Method of the Year 2016. A News Feature looks at the history of the field, from the first discoveries of RNA modifications in the 1960s to recent transcriptome-wide methods. A Review describes the strengths and weaknesses of these methods, and a Commentary discusses the functional importance of a particular modification in stem cells. Our choice of eight methods to watch highlights areas we think will be influential in 2017 and beyond.

January Vol. 14, No. 1

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2016

Technology Features

Collection: Technology Features

A selection of Technology Features published in Nature and Nature Methods over the past year highlights topics in imaging, tissue clearing and probe finessing, genomics, epigenomics and microbiology, connectomics, proteomics, immunology, polymerases and cell-based assays in plant biology.

Collection delivered by Zmags

Method of the Year 2015

Special Feature — Method of the Year 2015

Our choice for Method of the Year 2015 is single-particle cryo-electron microscopy. A collection of articles discusses how recent technical advances, especially the development of direct-detection cameras, have enabled this structural biology technique to make impressive leaps in achievable resolution and, in turn, provide new insights about protein function. We also highlight methods to watch in the upcoming years.

January Vol. 13, No. 1

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2015

Method of the Year 2014

Special Feature — Method of the Year 2014

Our Method of the Year 2014 goes to light-sheet fluorescence microscopy. This series of papers discusses how this technology, in combination with increasingly sophisticated cameras and powerful computing, is dramatically changing and enabling the imaging of living biological samples from developing embryos to functioning brains. We also highlight methods worth watching in the upcoming years.

January Vol. 12, No. 1

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2014

Nobel Prize in Chemistry 2014

Collection: Nobel Prize in Chemistry 2014

The 2014 Nobel Prize in Chemistry was awarded for the development of superresolution fluorescence microscopy, which enables the imaging of fine biological structures previously thought to be unresolvable using light. This collection of news pieces and articles by the Nobel laureates and their collaborators celebrates this achievement.

Ten Year Anniversary Special

Ten Year Anniversary Special

Nature Methods is ten years old. In this anniversary issue, we highlight our choice of the ten areas of methods development with the most impact on biological research over the past decade, and feature commentaries on a subset of these methods.

Visit Methagora to browse Nature Methods papers in these areas.

October Vol. 11, No. 10

Technology Features 2014

Collection: Technology Features

A selection of Technology Features published in Nature and Nature Methods over the past year highlights topics in super-resolution microscopy, cell-line authentication, when to choose cell lines versus model organisms, near-infrared fluorescent proteins, PCR, liquid handling, noncoding RNAs, genome assembly, de novo mutation analysis tools, approaches to discern driver from passenger mutations, new technology to map the brain in high-resolution and crowdsourcing in neuroscience.

Collection delivered by Zmags

Method of the Year 2013

Focus on Synthetic Biology

Synthetic biology spans many disciplines and has many different goals but all rely on well-characterized basic tools. In this Focus experts discuss the current status of essential methods, going from DNA synthesis to genetic circuit design to whole genome assembly and how these tools can be deployed for applications from medical research to addressing the origin of life.

May Vol. 11, No. 5

Method of the Year 2013

Special Feature — Method of the Year 2013

Nature Methods' choice for Method of the Year 2013 is single-cell sequencing. A collection of articles present the unique considerations related to sequencing single cells and highlight recent applications in biology and medicine. The Methods to Watch feature provides a look at possible future Methods of the Year.

January Vol. 11, No. 1

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2013

Light-sheet Microscopy

Collection: Light-sheet Microscopy

The use of a planar sheet of light for illumination in fluorescence microscopy allows researchers to image sample volumes faster than possible with other current methods, while limiting light dosage. This collection of articles provides a brief overview of this exciting methodology and the biological research applications that it makes possible.

Technology Features 2013

Collection: Technology Features

A selection of Technology Features published in Nature and Nature Methods over the past year highlights topics in microscopy, antibodies, cell heterogeneity, epigenetics, genome editing, post-translational modifications, PCR, stem cell–niche engineering, cell culture media, in vivo imaging, synthetic biology and genome analysis workflows and tools.

Collection delivered by Zmags

Focus on Mapping the Brain

Focus on Mapping the Brain

We are entering a new era in the neurosciences, in which development of technology will be in the spotlight. In this Focus, experts outline the different technologies needed to obtain anatomical and functional brain maps across species, and discuss the importance of assembling these maps and what will be needed beyond them, to understand the functioning of the brain.

June Vol. 10, No. 6

Method of the Year 2011

Special Feature — Method of the Year 2012

Nature Methods' choice for Method of the Year 2012 is targeted proteomics. A collection of articles showcases how mass spectrometry technology is being developed and applied for targeted protein analysis in both basic and translational research.

January Vol. 10, No. 1

2012

Technology Features 2012

Collection: Technology Features

A selection of Technology Features published in Nature and Nature Methods over the past year highlights topics in microscopy, genome sequencing, digital PCR, mass spectrometry, protein interactions, epigenetics, optogenetics and data handling.

Collection delivered by Zmags

Focus on Bioimage Informatics

Focus on Bioimage Informatics

Explosive growth in the size and complexity of microscopy-based imaging data and the need to extract more quantitative data from it increasingly requires sophisticated image acquisition and analysis methods and software tools. A collection of articles discusses the role of bioimage informatics in microscopy, specific tools that are available, and the challenges and opportunities in the field.

July Vol. 9, No. 7

Method of the Year 2011

Special Feature — Method of the Year 2011

Nature Methods' choice for Method of the Year 2011 is genome editing with engineered nucleases. This collection of articles—and the related video—highlights how the ability to use engineered nucleases to make precise, tailored and specific changes to coding and noncoding sequences of the genome, in cells and in organisms of many species, could revolutionize the study of gene function.

January Vol. 9, No. 1

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2011

Technology Features 2011

Collection: Technology Features

A selection of Technology Features published in Nature and Nature Methods over the past year highlights topics in nucleic acid sequencing and synthesis, microscopy, mass spectrometry, cell and tissue culture, whole-organism phenotyping and laboratory software.

Collection delivered by Zmags

International Year of Chemistry

Special Feature — International Year of Chemistry

We celebrate the 2011 International Year of Chemistry by highlighting the important contributions of chemistry to methods currently used in biology research. In a series of Commentaries, developers of chemistry-related tools and methods in three selected areas of research discuss their history and applications.

August Vol. 8, No. 8

Flow-Cytometry

Collection: Flow Cytometry

Flow cytometry has been an essential technique in modern biological research and the number and variety of applications using flow cytometers has been expanding rapidly. This collection of articles highlights recent methodological advances in the use of flow cytometry and the powerful new applications being developed.

Single-cell Analysis

Single-cell Analysis

In a series of commissioned pieces, authors discuss methods for the analysis of single cells and consider technical developments still needed.

April Vol. 8, No. 4s

Method of the Year 2010

Method of the Year 2010

Nature Methods' choice of Method of the Year 2010 is optogenetics for its capacity to control cell function with light. A series of articles and a video describe how optogenetics has revolutionized the way experiments are conducted in neuroscience and showcase the potential the method has for the study of many signaling pathways in cell biology. The special feature also discusses how technological development will be needed to expand the possibilities of optogenetics.

January Vol. 8, No. 1

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2010

Super-resolution Microscopy

Collection: Super-resolution Microscopy

Fluorescence microscopy is acquiring new capabilities as methodological developments allow it to break the diffraction limit. This collection of articles from several leaders in the field highlights the diversity of super-resolution microscopy techniques being developed and the principles that allow them to overcome this long-standing limitation.

Visualizing Biological Data

Visualizing Biological Data

A series of five commissioned Reviews discuss the challenges of visualizing biological data and the visualization tools available to biologists working with genomes, alignments and phylogenies, macromolecular structures, images and systems biology data.

March Vol. 7, No. 3s

Method of the Year 2009

Method of the Year 2009

Nature Methods' Method of the Year 2009 goes to induced pluripotency for its potential for biological discovery. This series of articles—and the related video—showcase how induced pluripotency is coming into its own in 2009 as a tool for discovery in both basic and disease biology and explore the incredible impact this area promises to have in biological research.

January Vol. 7, No. 1

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2009

Next-generation sequencing data analysis

Next-generation sequencing data analysis

A commissioned Commentary discusses the strength and challenges of next-generation sequencing, and a series of commissioned Reviews explain the principles behind data-analysis software for important applications from alignment and assembly to structural-variant detection and the interpretation of ChIP-seq and RNA-seq data.

October Vol. 6, No. 11s

Five Year Anniversary Special

Special Feature - Five Year Anniversary Special

Nature Methods celebrates its five year anniversary with commentaries discussing the impact and progress of methodological developments in the life sciences. We also include a fun selection of papers and covers from our pages.

October Vol. 6, No. 10

Method of the Year 2008

Special Feature - Method of the Year 2008

Nature Methods' Method of the Year 2008 goes to super-resolution fluorescence microscopy. This series of articles—and the related movie—showcase how these novel imaging methods came into their own in 2008 and the incredible impact they promise to have in biological research.

January Vol. 6, No. 1

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2008

Focus on Single-Molecule Analysis

Focus on Single-Molecule Analysis

Specially commissioned Reviews and a Perspective provide a guide to the maturing field of single-molecule analysis and two original research papers describe complementary advances in nanoscale visualization.

June Vol. 5, No. 6

Special Feature - Method of the Year 2007

Special Feature - Method of the Year 2007

Nature Methods' Method of the Year 2007 goes to next-generation sequencing. This series of articles showcase how these novel sequencing methods came into their own in 2007 and the incredible impact they promise to have in a variety of research applications. The Methods to Watch feature provide a glimpse and a wish list for future Methods of the Year.

January Vol. 5, No. 1

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2007

Focus on RNA interference - A user's guide

Focus on mass spectrometry in proteomics

A series of specially commissioned Commentaries, Reviews and Perspectives discuss the use of mass spectrometry in established approaches to proteomics as well as emerging technologies.

October Vol. 4, No. 10

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2006

Focus on RNA interference - A user's guide

Focus on RNA interference - A user's guide

Eight especially commissioned Perspective articles that discuss practical questions surrounding the use of RNA interference in mammalian cells, as a tool to knock down a specific gene of interest and as a resource for functional genomics screens.

September Vol. 3, No. 9


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2005

Fluorescence Imaging

Fluorescence Imaging

A series of review articles and other content discussing the principles of fluorescence and different imaging modalities along with practical advice.

December Vol. 2, No. 12

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