Nature Methods - CURRENT ISSUE : December 2009 - Vol 6 No 12
- Improving genetically encoded Ca2+ indicators
- Tissue-specific microRNA sponges
- Rapid and reversible protein degradation
Latest highlights
FOCUS
NGS data analysis
Online FocusFREE
To extract a maximum of information from next-generation sequencing data, users have to navigate a maze of different software options. A series of Reviews guides readers through the analysis steps for different applications and explains how different algorithms handle these steps.
SUPPLEMENT
Nature Milestones in Light Microscopy
Nature MilestonesFREE
The Nature Milestones in Light Microscopy supplement presents a historical overview of the key developments in light microscopy. From the first light microscopes over 400 years ago, to super-resolution imaging in the last decade, these technological developments have changed the way biologists work and point to continued advances in the future. A timeline and collection of short articles highlight these important developments.
CURRENT ISSUE
Tissue-specific miRNA sponges
Article by Loya et al.Tissue-specific expression of microRNA sponges allows precise regulation of microRNA activity in living flies. Van Vactor and colleagues investigate the role of miR-8 in the formation of neuromuscular junctions.
CURRENT ISSUE
Controlling degradation
Article by Nishimura et al.Kanemaki and colleagues show that a degradation pathway found in plants, dependent on the hormone auxin, can be transplanted and harnessed to induce rapid and reversible target protein degradation in both yeast and animal cells.
CURRENT ISSUE
Making molecular patterns
Article by Kress et al.Microsources positioned with holographic optical tweezers can establish a highly localized, three-dimensional chemical gradient that, Dufresne and colleagues show, allows the manipulation of polarization and migration in single cells.
RESEARCH HIGHLIGHTS
Reprogramming the epiblast
Many routes to pluripotencyFREE
Cells from the epiblast of the post-implantation mouse embryo can be reprogrammed to pluripotency without the addition of exogenous genes. Unlike epiblast stem cells, these reprogrammed cells contribute to mouse chimeras and to the germline.
NEW ON METHAGORA
Neuroscience tools
MethagoraFREE
Technical improvements to optical probes and methods are making it possible to probe neural function in ways previously unimaginable. But improvements are still needed, and ways to standardize the evaluation of new tools will be important for future development. Visit our blog to read our editorial on the topic.
As always, we welcome your comments on this and other posts.
Application Notes
SENSOLUX® stand-alone version: noninvasive determination of pH and DO in shake flasks
Go to the Application Notes database
See also this month:
Application Notes from BioTek, Epicentre Biotechnologies, Thermo Scientific, febit GmbH, Invitrogen, and Glen Mills.
