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We provide a twisting fabrication process for fiber electrodes that can be assembled into electronic threads and then integrated in electronic textile-based wearables.
The authors describe how to prepare antimicrobial phage-based microgels, using polystyrene film templates, and detail their use to kill antibiotic-resistant bacteria in food contamination tests as an example application.
This protocol provides detailed guidelines for using rMATS-turbo—the latest implementation of the popular software for the discovery and quantification of alternative splicing events from RNA sequencing data—exemplified in two representative scenarios.
A protocol for Nano-DMS-MaP, a method for in situ isoform-specific RNA structure determination. The technique employs nanopore sequencing to resolve the structures of long and highly similar RNA molecules, revealing previously hidden structural differences.
The Protocol Exchange is an open repository of community-contributed protocols. See the latest, including microCT scanning and analysis in the zebrafish skeleton, assessment of sleep health in Iran: a nationwide study protocol, and DPC-seq: genome-wide mapping of DNA-protein crosslinks (DPC) induced by endogenous aldehydes.
Our authors are invited to write blog posts that describe how they conceived and developed their protocols, prior to publication at Nature Protocols. These stories are published on a community website for researchers who are interested in techniques and methods.
This Collection highlights articles on how to improve methodological clarity in science publications from a spectrum of different journals and subject areas.
Synteny and colinearity are important parameters that delineate the evolution of genomes and gene families. This protocol describes MCScanX, a user-friendly toolkit that facilitates rapid evolutionary analysis of chromosomal structural changes.
Transkingdom Network Analysis (TkNA) is a unique analytical framework for inferring causal factors underlying host–microbiota and other multi-omic interactions, by integrating data from multiple cohorts and diverse omics types.
Ultra-stable magnetic tweezers allow measuring individual protein dynamics in equilibrium under physiologically relevant pulling forces and over timescales of days to weeks, enabling high-precision molecular studies in mechanobiology.
Atomic force microscopy can be used to determine the stiffness of materials. This protocol describes how to measure and quantify the Young’s modulus E of pulmonary mouse and human basement membranes with atomic force microscopy and the Center for Applied Tissue Engineering and Regenerative Medicine processing toolbox.
We provide a twisting fabrication process for fiber electrodes that can be assembled into electronic threads and then integrated in electronic textile-based wearables.
The authors describe how to prepare antimicrobial phage-based microgels, using polystyrene film templates, and detail their use to kill antibiotic-resistant bacteria in food contamination tests as an example application.
Optimization of chemical transformations involves screening numerous reaction parameters. This protocol outlines how screening experiments can be rapidly and economically analyzed by quantitative benchtop 19F NMR spectroscopy.
Phylogenetic trees from whole-genome sequencing datasets with the pipeline Sequoia are reconstructed to define the evolutionary history of clonally expanding cells and to clarify how somatic mutations shape normal cell behavior.