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Cover Art: Primordial Elements by Eve Stockton www.evestockton.com
Gene silencing through RNA interference (RNAi) has become the tool of choice for genome-scale, high-throughput analyses of gene function and has had a tremendous impact on science and drug discovery. Now, RNAi libraries targeting whole genomes or functionally related pathways permit systematic screens for clinically relevant components of cellular networks. Advances in rational design, sequence selection strategies and delivery options have substantially improved the utility of short interfering RNA (siRNA), short hairpin RNA (shRNA) and microRNA (miRNA) tools for screening.
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Article
Identification of genes that regulate epithelial cell migration using an siRNA screening approach
Kaylene J. Simpson, Laura M. Selfors, James Bui, Angela Reynolds, Devin Leake, Anastasia Khvorova & Joan S. Brugge
Nature Cell Biology 10, 1027—1038 (2008)
doi:10.1038/ncb1762
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Letters
Embryonic stem cell-specific microRNAs regulate the G1-S transition and promote rapid proliferation
Yangming Wang, Scott Baskerville, Archana Shenoy, Joshua E Babiarz, Lauren Baehner & Robert Blelloch
Nature Genetics 40, 1478—1483 (2008)
doi:10.1038/ng.250
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Review
Statistical methods for analysis of high-throughput RNA interference screens
Amanda Birmingham, Laura M Selfors, Thorsten Forster, David Wrobel, Caleb J Kennedy, Emma Shanks, Javier Santoyo-Lopez, Dara J Dunican, Aideen Long, Dermot Kelleher, Queta Smith, Roderick L Beijersbergen, Peter Ghazal & Caroline E Shamu
Nature Methods 6, 569—575 (2009)
doi:10.1038/nmeth.1351
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Letters
An elaborate pathway required for Ras-mediated epigenetic silencing
Claude Gazin, Narendra Wajapeyee, Stephane Gobeil, Ching-Man Virbasius & Michael R. Green
Nature 449, 1073—1077 (2007)
doi:10.1038/nature06251
Synthetic lethal screen identification of chemosensitizer loci in cancer cells
Angelique W. Whitehurst, Brian O. Bodemann, Jessica Cardenas, Deborah Ferguson, Luc Girard, Michael Peyton, John D. Minna, Carolyn Michnoff, Weihua Hao, Michael G. Roth, Xian-Jin Xie & Michael A. White
Nature 446, 815—819 (2007)
doi:10.1038/nature05697
A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity
Na-Yu Chia, Yun-Shen Chan, Bo Feng, Xinyi Lu, Yuriy L. Orlov, Dimitri Moreau, Pankaj Kumar, Lin Yang, Jianming Jiang, Mei-Sheng Lau, Mikael Huss, Boon-Seng Soh, Petra Kraus, Pin Li, Thomas Lufkin, Bing Lim, Neil D. Clarke, Frederic Bard & Huck-Hui Ng
Nature 468, 316—320 (2010)
doi:10.1038/nature09531
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