Super-resolution Microscopy
Fluorescence microscopy is acquiring new capabilities as methodological developments allow it to break the diffraction limit—a barrier that until recently prevented light microscopy from being used to interrogate details of cellular function beyond the resolving power of conventional light microscopy. This collection of articles from several leaders in the field highlights the diversity of super-resolution microscopy techniques being developed and the principles that allow them to overcome this long-standing limitation.
Top of pageEditorial
Editorial
Microscopy begets nanoscopy
Daniel Evanko
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Top of pageSuper-resolution microscopy collection
Primer
Primer: fluorescence imaging under the diffraction limit
Daniel Evanko
Nature Methods 6, 19—20 (2009)
doi:10.1038/nmeth.f.235
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Perspective
Microscopy and its focal switch
Stefan W Hell
Nature Methods 6, 24—32 (2009)
doi:10.1038/nmeth.1291
Abstract | Full Text | PDF
Commentary
Putting super-resolution fluorescence microscopy to work
Jennifer Lippincott-Schwartz & Suliana Manley
Nature Methods 6, 21—23 (2009)
doi:10.1038/nmeth.f.233
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Commentary
Subdiffraction resolution in continuous samples
Rainer Heintzmann & Mats G L Gustafsson
Nature Photonics 3, 362—364 (2009)
doi:10.1038/nphoton.2009.102
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News and Views
Single-molecule mountains yield nanoscale cell images
W E Moerner
Nature Methods 3, 781—782 (2006)
doi:10.1038/nmeth1006-781
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Article
Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics
Hari Shroff, Catherine G Galbraith, James A Galbraith & Eric Betzig
Nature Methods 5, 417—423 (2008)
doi:10.1038/nmeth.1202
Abstract | Full Text | PDF
Article
Spherical nanosized focal spot unravels the interior of cells
Roman Schmidt, Christian A Wurm, Stefan Jakobs, Johann Engelhardt, Alexander Egner & Stefan W Hell
Nature Methods 5, 539—544 (2008)
doi:10.1038/nmeth.1214
Abstract | Full Text | PDF
Article
Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution
Bo Huang, Sara A Jones, Boerries Brandenburg & Xiaowei Zhuang
Nature Methods 5, 1047—1052 (2008)
doi:10.1038/nmeth.1274
Abstract | Full Text | PDF
Brief Communication
Super-resolution video microscopy of live cells by structured illumination
Peter Kner, Bryant B Chhun, Eric R Griffis, Lukman Winoto & Mats G L Gustafsson
Nature Methods 6, 339—342 (2009)
doi:10.1038/nmeth.1324
Abstract | Full Text | PDF