Super-resolution microscopy is poised to revolutionize our understanding of the workings of the cell. But the technology still has some limitations, and these must be taken into consideration if widespread application is to yield biological insight.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Optimized measurements of separations and angles between intra-molecular fluorescent markers
Nature Communications Open Access 16 October 2015
-
Three-dimensional super-resolution protein localization correlated with vitrified cellular context
Scientific Reports Open Access 14 October 2015
-
Bayesian localization microscopy based on intensity distribution of fluorophores
Protein & Cell Open Access 12 February 2015
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Betzig, E., Trautman, J.K., Harris, T.D., Weiner, J.S. & Kostelak, R.L. Science 251, 1468–1470 (1991).
Hell, S.W. & Wichmann, J. Opt. Lett. 19, 780–782 (1994).
Gustafsson, M.G. Proc. Natl. Acad. Sci. USA 102, 13081–13086 (2005).
Betzig, E. et al. Science 313, 1642–1645 (2006).
Rust, M.J., Bates, J. & Zhuang, X. Nat. Methods 3, 793–796 (2006).
Hess, S.T., Girirajan, T.P.K. & Mason, M.D. Biophys. J. 91, 4258–4272 (2006).
Schermelleh, L. et al. Science 320, 1332–1336 (2008).
Willig, K.I., Rizzoli, S.O., Westphal, V., Jahn, R. & Hell, S.W. Nature 440, 935–939 (2006).
Kittel, R.J. et al. Science 312, 1051–1054 (2006).
Huang, B., Wang, W., Bates, M. & Zhuang, X. Science 319, 810–813 (2008).
Shroff, H., Galbraith, C.G., Galbraith, J.A. & Betzig, E. Nat. Methods 5, 417–423 (2008).
Biteen, J.S. et al. Nat. Methods (in the press).
Manley, S. et al. Nat. Methods 5, 155–157 (2008).
Shannon, C.E. Proc. Inst. Radio Eng. 37, 10–21 (1949). Reprinted in Proc. IEEE 86, 447–457 (1998).
Shroff, H. et al. Proc. Natl. Acad. Sci. USA 104, 20308–20313 (2007).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lippincott-Schwartz, J., Manley, S. Putting super-resolution fluorescence microscopy to work. Nat Methods 6, 21–23 (2009). https://doi.org/10.1038/nmeth.f.233
Published:
Issue Date:
DOI: https://doi.org/10.1038/nmeth.f.233
This article is cited by
-
Diverse protocols for correlative super-resolution fluorescence imaging and electron microscopy of chemically fixed samples
Nature Protocols (2017)
-
Image artifacts in Single Molecule Localization Microscopy: why optimization of sample preparation protocols matters
Scientific Reports (2015)
-
Optimized measurements of separations and angles between intra-molecular fluorescent markers
Nature Communications (2015)
-
Three-dimensional super-resolution protein localization correlated with vitrified cellular context
Scientific Reports (2015)
-
Bayesian localization microscopy based on intensity distribution of fluorophores
Protein & Cell (2015)