Brief Communication abstract
Nature Methods 6, 339 - 342 (2009)
Published online: 26 April 2009 | doi:10.1038/nmeth.1324
Super-resolution video microscopy of live cells by structured illumination
Peter Kner1,2,7,8, Bryant B Chhun1,8, Eric R Griffis3,4, Lukman Winoto5 & Mats G L Gustafsson5,6
Structured-illumination microscopy can double the resolution of the widefield fluorescence microscope but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed structured-illumination microscope that is capable of 100-nm resolution at frame rates up to 11 Hz for several hundred time points. We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila melanogaster S2 cells in the total internal reflection mode.
- Department of Biochemistry and Biophysics, San Francisco California, USA.
- Keck Advanced Microscopy Laboratory, San Francisco California, USA.
- Department of Cellular and Molecular Pharmacology, San Francisco California, USA.
- Howard Hughes Medical Institute, San Francisco California, USA.
- Department of Physiology and Program in Bioengineering, University of California San Francisco, San Francisco California, USA.
- Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA.
- Present address: Faculty of Engineering, University of Georgia, Athens, Georgia, USA.
- These authors contributed equally to this work.
Correspondence to: Mats G L Gustafsson5,6 e-mail: gustafssonm@janelia.hhmi.org
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