Developmental biology

  • Article |

    Small intestinal crypts contain twice as many effective stem cells as large intestinal crypts, and this difference is determined by the degree of Wnt-driven retrograde cell movement—which is largely absent in the large intestine—counteracting conveyor-belt-like upward movement.

    • Maria Azkanaz
    • , Bernat Corominas-Murtra
    •  & Jacco van Rheenen
  • Article
    | Open Access

    This study provides a comprehensive spatiotemporal map of human and mouse gonadal differentiation, using a combination of single-cell and spatial transcriptomics, chromatin accessibility assays and fluorescent microscopy, which can guide in vitro gonadogenesis.

    • Luz Garcia-Alonso
    • , Valentina Lorenzi
    •  & Roser Vento-Tormo
  • Article |

    3D transcriptomes reveal the molecular code of lineage specification in the primate embryo and provide an in vivo reference to decipher human development.

    • Sophie Bergmann
    • , Christopher A. Penfold
    •  & Thorsten E. Boroviak
  • Article |

    In situ barcoding and fate mapping in mice reveals that an early wave of progenitor specification, driven by embryonic multipotent progenitor cells, gives rise to adult blood independently of haematopoietic stem cells.

    • Sachin H. Patel
    • , Constantina Christodoulou
    •  & Fernando D. Camargo
  • Article |

    A study of zebrafish identifies an innate mechanism of blood vessel formation through the transdifferentiation of lymphatic endothelial cells, and provides in vivo evidence for a link between cell ontogeny and functionality in endothelial cells.

    • Rudra N. Das
    • , Yaara Tevet
    •  & Karina Yaniv
  • Article |

    Characterization of Gibbin, encoded by AHDC1, offers insights into the epidermal and mesodermal patterning phenotypes seen in Xia–Gibbs and related syndromes in humans, which derive from abnormal mesoderm maturation as a result of gene-specific DNA methylation decisions.

    • Ann Collier
    • , Angela Liu
    •  & Anthony E. Oro
  • Article |

    In the tunicate Ciona intestinalis, the homeobox transcription factor Hmx drives differentiation of bipolar tail neurons, indicating conserved regulation and function, and homology with cranial sensory ganglia in higher vertebrates.

    • Vasileios Papadogiannis
    • , Alessandro Pennati
    •  & Sebastian M. Shimeld
  • Article
    | Open Access

    Metabolomics analysis of the mouse embryo shows a metabolic shift towards the tricarboxylic acid cycle between gestational days 10.5 and 11.5, leading to the subsequent development of organ-specific metabolic programmes.

    • Ashley Solmonson
    • , Brandon Faubert
    •  & Ralph J. DeBerardinis
  • Article |

    Spatial transcriptomics and single-cell profiling identify previously uncharacterized cell types of human terminal and respiratory bronchioles, and show that cell differentiation and lineage trajectories are distinct from those in the mouse lung.

    • Preetish Kadur Lakshminarasimha Murthy
    • , Vishwaraj Sontake
    •  & Purushothama Rao Tata
  • Article
    | Open Access

    Evolutionary modelling shows that an initial set of inhibitory neurons serving olfactory bulbs may have been repurposed to diversify the taxonomy of interneurons found in the expanded striata and cortices in primates.

    • Matthew T. Schmitz
    • , Kadellyn Sandoval
    •  & Alex A. Pollen
  • Article |

    Haploinsufficiency in three genes associated with risk of autism spectrum disorder—KMT5B, ARID1B and CHD8—in cell lines from multiple donors results in cell-type-specific asynchronous development of GABAergic neurons and cortical deep-layer excitatory projection neurons.

    • Bruna Paulsen
    • , Silvia Velasco
    •  & Paola Arlotta
  • Article |

    Experiments in cerebral organoids show that sex hormones have a role in regulating the number of excitatory neurons in the human neocortex, providing insight into the mechanistic basis of sex-related brain differences in humans.

    • Iva Kelava
    • , Ilaria Chiaradia
    •  & Madeline A. Lancaster
  • Article |

    A chromatin accessibility atlas of 240,919 cells in the adult and developing Drosophila brain reveals 95,000 enhancers, which are integrated in cell-type specific enhancer gene regulatory networks and decoded into combinations of functional transcription factor binding sites using deep learning.

    • Jasper Janssens
    • , Sara Aibar
    •  & Stein Aerts
  • Article |

    A mechanism involving intracellular recycling of the morphogen Decapentaplegic (Dpp) underlies the scaling of the Dpp gradient in the Drosophila wing disc, and this is modulated by the extracellular factors Pentagone and Dally.

    • Maria Romanova-Michaelides
    • , Zena Hadjivasiliou
    •  & Marcos Gonzalez-Gaitan
  • Article |

    Following global DNA demethylation, mouse gonadal primordial germ cells undergo remodelling of repressive chromatin modifications, resulting in a sex-specific signature that is required to safeguard the transcriptional program.

    • Tien-Chi Huang
    • , Yi-Fang Wang
    •  & Petra Hajkova
  • Article
    | Open Access

    Blastoids derived from naive PXGL-cultured human pluripotent stem cells in which Hippo, TGF-β and ERK pathways are inhibited closely recapitulate aspects of blastocyst development, form cells resembling blastocyst-stage cells and thus provide a model system for implantation and development studies.

    • Harunobu Kagawa
    • , Alok Javali
    •  & Nicolas Rivron
  • Article |

    The single-cell transcriptional profile of a human embryo between 16 and 19 days after fertilization reveals parallels and differences in gastrulation in humans as compared with mouse and non-human primate models.

    • Richard C. V. Tyser
    • , Elmir Mahammadov
    •  & Shankar Srinivas
  • Article |

    In the post-mitotic nervous system of Caenorhabditis elegans, the promotion of a mature neuronal transcription program is controlled by the developmental-stage-specific upregulation of the microRNA LIN-4 and the subsequent repression of its target, the transcription factor lin-14.

    • HaoSheng Sun
    •  & Oliver Hobert
  • Article |

    Cryo-electron microscopy studies show that dynamic coordination of Na+ in the ion channel of Dispatched homologue 1 and the transmembrane Na+ gradient have key roles in exporting lipid-modified Hedgehog protein signal.

    • Qianqian Wang
    • , Daniel E. Asarnow
    •  & Philip A. Beachy
  • Article
    | Open Access

    Auxin induces transmembrane-kinase-dependent activation of H+-ATPase in the plasma membrane through phosphorylation of its penultimate threonine residue, promoting apoplastic acidification and hypocotyl cell elongation in Arabidopsis.

    • Wenwei Lin
    • , Xiang Zhou
    •  & Zhenbiao Yang
  • Article
    | Open Access

    RNA-sequencing analysis of the prenatal human brain at different stages of development shows that areal transcriptional signatures are dynamic and coexist with developmental and cell-type signatures.

    • Aparna Bhaduri
    • , Carmen Sandoval-Espinosa
    •  & Arnold R. Kriegstein
  • Article |

    A single-cell atlas of human fetal bone marrow in healthy fetuses and fetuses with Down syndrome provides insight into developmental haematopoiesis in humans and the transcription and functional differences that occur in Down syndrome.

    • Laura Jardine
    • , Simone Webb
    •  & Muzlifah Haniffa
  • Perspective |

    This Perspective outlines the Human Developmental Cell Atlas initiative, which uses state-of-the-art technologies to map and model human development across gestation, and discusses the early milestones that have been achieved.

    • Muzlifah Haniffa
    • , Deanne Taylor
    •  & Matthias Zilbauer
  • Article
    | Open Access

    Cells from embryonic, fetal, paediatric and adult human intestinal tissue are analysed at different locations along the intestinal tract to construct a single-cell atlas of the developing and adult human intestinal tract, encompassing all cell lineages.

    • Rasa Elmentaite
    • , Natsuhiko Kumasaka
    •  & Sarah A. Teichmann
  • Article |

    Serial-section electron microscopy is used to reconstruct the full brain connectome of eight individual Caenorhabditis elegans at various stages of development, providing insight into the principles underlying brain maturation.

    • Daniel Witvliet
    • , Ben Mulcahy
    •  & Mei Zhen
  • Article |

    A comprehensive single-cell transcriptomic atlas of the mouse brain between gastrulation and birth identifies hundreds of cellular states and reveals the spatiotemporal organization of brain development.

    • Gioele La Manno
    • , Kimberly Siletti
    •  & Sten Linnarsson