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Reconstruction and deconstruction of human somitogenesis in vitro
Somitoids and segmentoids—culture systems that recapitulate the formation of somite-like structures—reveal that an initial salt-and-pepper expression pattern of MESP2 in a newly formed segment is transformed into compartments of anterior and posterior identity through an active cell-sorting mechanism.
- Yuchuan Miao
- , Yannis Djeffal
- & Olivier Pourquié
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Article |
Periodic inhibition of Erk activity drives sequential somite segmentation
The zebrafish segmentation clock drives sequential segmentation of somites by periodically lowering double-phosphorylated Erk and therefore projecting its oscillation on the double-phosphorylated Erk gradient.
- M. Fethullah Simsek
- , Angad Singh Chandel
- & Ertuğrul M. Özbudak
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Article |
Gibbin mesodermal regulation patterns epithelial development
Characterization of Gibbin, encoded by AHDC1, offers insights into the epidermal and mesodermal patterning phenotypes seen in Xia–Gibbs and related syndromes in humans, which derive from abnormal mesoderm maturation as a result of gene-specific DNA methylation decisions.
- Ann Collier
- , Angela Liu
- & Anthony E. Oro
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Article |
Anatomically distinct fibroblast subsets determine skin autoimmune patterns
Single-cell analyses of skin samples from patients with vitiligo and functional genetic experiments in vitiligo mouse models show that distinct fibroblast subsets drive the organ level lesion patterns in this autoimmune disease.
- Zijian Xu
- , Daoming Chen
- & Ting Chen
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Article |
Single-cell transcriptomic characterization of a gastrulating human embryo
The single-cell transcriptional profile of a human embryo between 16 and 19 days after fertilization reveals parallels and differences in gastrulation in humans as compared with mouse and non-human primate models.
- Richard C. V. Tyser
- , Elmir Mahammadov
- & Shankar Srinivas
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Article |
Dispatched uses Na+ flux to power release of lipid-modified Hedgehog
Cryo-electron microscopy studies show that dynamic coordination of Na+ in the ion channel of Dispatched homologue 1 and the transmembrane Na+ gradient have key roles in exporting lipid-modified Hedgehog protein signal.
- Qianqian Wang
- , Daniel E. Asarnow
- & Philip A. Beachy
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Article |
Pairing of segmentation clock genes drives robust pattern formation
The pairing of genes is essential for robust expression of segmentation clock genes during development in zebrafish embryos.
- Oriana Q. H. Zinani
- , Kemal Keseroğlu
- & Ertuğrul M. Özbudak
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Article |
Glypicans shield the Wnt lipid moiety to enable signalling at a distance
Genetic studies in Drosophila combined with structural analyses show that glypicans bind palmitoylate moieties in Wnt ligands, and thus shield Wnt ligands from their aqueous environments to enable them to signal to their distant receptors.
- Ian J. McGough
- , Luca Vecchia
- & Jean-Paul Vincent
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An in vitro model of early anteroposterior organization during human development
Human gastruloids—three-dimensional aggregates derived from human embryonic stem cells—show features of human embryos at around 19–21 days, and provide a model for the study of early human development.
- Naomi Moris
- , Kerim Anlas
- & Alfonso Martinez Arias
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Article |
Single-cell and spatial transcriptomics reveal somitogenesis in gastruloids
Single-cell RNA sequencing and spatial transcriptomics reveal that the somitogenesis clock is active in mouse gastruloids, which can be induced to generate somites with the correct rostral–caudal patterning.
- Susanne C. van den Brink
- , Anna Alemany
- & Alexander van Oudenaarden
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Article |
In vitro characterization of the human segmentation clock
Human presomitic mesoderm cells derived in vitro demonstrate oscillations of the segmentation clock, thus providing a window into an otherwise inaccessible stage of human development.
- Margarete Diaz-Cuadros
- , Daniel E. Wagner
- & Olivier Pourquié
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Article |
Coupling delay controls synchronized oscillation in the segmentation clock
Monitoring cells of the mouse presomitic mesoderm using the Achilles reporter fused to HES7 sheds light on the mechanisms that underpin synchronous oscillations in the expression of clock genes between neighbouring cells.
- Kumiko Yoshioka-Kobayashi
- , Marina Matsumiya
- & Ryoichiro Kageyama
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Letter |
Wnt and TGFβ coordinate growth and patterning to regulate size-dependent behaviour
A planarian fission protocol shows that the number of progeny and the frequency of fission initiation correlate with parent size, and TGFβ and Wnt signalling components are identified as regulators of fission behaviour.
- Christopher P. Arnold
- , Blair W. Benham-Pyle
- & Alejandro Sánchez Alvarado
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Letter |
Hydraulic control of mammalian embryo size and cell fate
A mouse blastocyst model reveals how lumenal pressure, cell cortical tension and tissue stiffness act at the tissue scale to regulate embryo size, which in turn influences the division pattern of trophectoderm cells and their fate specification.
- Chii Jou Chan
- , Maria Costanzo
- & Takashi Hiiragi
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Letter |
Self-organization of a human organizer by combined Wnt and Nodal signalling
Stimulation of Wnt and Nodal pathways in micropatterned human embryonic stem cell colonies induce these colonies to exhibit characteristic spatial expression patterns of the organizer and reproduce organizer function when grafted into a host embryo.
- I. Martyn
- , T. Y. Kanno
- & A. H. Brivanlou
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Letter |
Orthogonal muscle fibres have different instructive roles in planarian regeneration
Longitudinal and circular muscle fibres have distinct regulatory roles during planarian regeneration.
- M. Lucila Scimone
- , Lauren E. Cote
- & Peter W. Reddien
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Article |
Wnt/β-catenin promotes gastric fundus specification in mice and humans
Wnt signalling is shown to be required for specification of the gastric fundus in mice, and was used to develop human gastric organoids with functional fundic cell types.
- Kyle W. McCracken
- , Eitaro Aihara
- & James M. Wells
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Letter |
Early myeloid lineage choice is not initiated by random PU.1 to GATA1 protein ratios
Live imaging and single-cell analyses are used to show that decision-making by differentiating haematopoietic stem cells between the megakaryocytic–erythroid and granulocytic–monocytic lineages is not initiated by stochastic switching between the lineage-specific transcription factors PU.1 and GATA1, which challenges the previous model of early myeloid lineage choice.
- Philipp S. Hoppe
- , Michael Schwarzfischer
- & Timm Schroeder
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Letter |
Molecular logic behind the three-way stochastic choices that expand butterfly colour vision
Butterflies diversify their retinal mosaics by producing three stochastic types of ommatidia instead of the two types found in Drosophila; this study shows that butterfly retinas use two R7-like photoreceptors per ommatidium that each make an independent stochastic decision to express the transcription factor Spineless, which controls photoreceptor and ommatidial fate.
- Michael Perry
- , Michiyo Kinoshita
- & Claude Desplan
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Letter |
FGF8 and SHH substitute for anterior–posterior tissue interactions to induce limb regeneration
The long-standing puzzle of why salamander limb regeneration requires anterior and posterior tissue interaction has been solved by the demonstration that fibroblast growth factor 8 and sonic hedgehog are key anterior and posterior cross-inductive signals that drive regeneration.
- Eugeniu Nacu
- , Elena Gromberg
- & Elly M. Tanaka
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Article |
Dpp spreading is required for medial but not for lateral wing disc growth
The morphogen Decapentaplegic (Dpp) has been implicated in both wing patterning and growth in fruitflies; here, a nanobody-based morphotrap approach has been developed that rules out a role for the Dpp gradient in regulating lateral wing growth.
- Stefan Harmansa
- , Fisun Hamaratoglu
- & Emmanuel Caussinus
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Letter |
Decapentaplegic and growth control in the developing Drosophila wing
The morphogen Decapentaplegic (Dpp) has been implicated in both wing patterning and growth in fruitflies; here, a CRISPR–Cas9 approach is used to rule out the Dpp gradient driving wing growth.
- Takuya Akiyama
- & Matthew C. Gibson
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Letter |
The ESCRT machinery regulates the secretion and long-range activity of Hedgehog
A new role for the endosomal sorting complex required for transport (ESCRT) is identified in fly larvae, where it is shown to be essential for the secretion and long-range signalling of the embryonic development morphogen Hedgehog.
- Tamás Matusek
- , Franz Wendler
- & Pascal P. Thérond
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Article |
RNA regulons in Hox 5′ UTRs confer ribosome specificity to gene regulation
Specialized ribosomes (with a particular protein composition) carry out translation of specific transcripts; analysis of Hox mRNA translation in mice reveals that unique RNA structural elements within their 5′ UTRs, including internal ribosome entry sites and translation inhibitory elements, are responsible for this specialized mode of translation.
- Shifeng Xue
- , Siqi Tian
- & Maria Barna
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Letter |
Calcisponges have a ParaHox gene and dynamic expression of dispersed NK homeobox genes
A study of the genomes of calcisponges shows that they contain at least one ParaHox gene, adding weight to the ‘ghost locus’ hypothesis that Hox and ParaHox genes were present in the earliest animal ancestor, but subsequently lost in some sponge lineages.
- Sofia A. V. Fortunato
- , Marcin Adamski
- & Maja Adamska
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Letter |
A Hox regulatory network of hindbrain segmentation is conserved to the base of vertebrates
Nested Hox expression domains are found in jawed vertebrates and in non-vertebrate chordates, but it is unclear whether there is a link between brain regionalization and Hox expression in jawless vertebrates; here, Hox expression is shown to be integrated with hindbrain segmentation in lampreys.
- Hugo J. Parker
- , Marianne E. Bronner
- & Robb Krumlauf
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Article |
Patterning and growth control by membrane-tethered Wingless
Replacement of the wingless (wg) gene in Drosophila with one that expresses a membrane-tethered form of Wg results in viable flies with normally patterned appendages of nearly the right size; early wg transcription and memory of signalling ensure continued target-gene expression in the absence of Wg release, even though the spread of Wg could boost cell proliferation.
- Cyrille Alexandre
- , Alberto Baena-Lopez
- & Jean-Paul Vincent
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Letter |
Specialized filopodia direct long-range transport of SHH during vertebrate tissue patterning
This study uses single-cell real-time imaging to show that sonic hedgehog (SHH) is produced in the form of a particle that is transported along a novel class of specialized actin-based filopodia spanning several cell diameters within the field of SHH cell signalling, thus expanding our knowledge of ligand movement during normal vertebrate development.
- Timothy A. Sanders
- , Esther Llagostera
- & Maria Barna
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Letter |
Scaling of embryonic patterning based on phase-gradient encoding
An ex vivo primary culture assay is developed that recapitulates mouse embryonic mesodermal patterning and segment formation; using this approach, it is shown that oscillating gene activity is central to maintain stable proportions during development.
- Volker M. Lauschke
- , Charisios D. Tsiairis
- & Alexander Aulehla
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Research Highlights |
Stripes from shifting cells
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Research Highlights |
Evolutionary biology: Leopards change their spots
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Letter |
Pericytes regulate the blood–brain barrier
The blood–brain barrier (BBB) is made up of vascular endothelial cells and was thought to have formed postnatally from astrocytes. Two independent studies demonstrate that this barrier forms during embryogenesis, with pericyte/endothelial cell interactions being critical to regulate the BBB during development. A better understanding of the relationship among pericytes, neuroendothelial cells and astrocytes in BBB function will contribute to our understanding of BBB breakdown during central nervous system injury and disease.
- Annika Armulik
- , Guillem Genové
- & Christer Betsholtz
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Letter |
Spatiotemporal regulation of cell-cycle genes by SHORTROOT links patterning and growth
In higher animals and plants, the processes of growth and patterning are coordinated. In this study, the authors study patterning in Arabidopsis root and show that two key regulators of root organ patterning directly control the transcription of specific components of the cell-cycle machinery. This study provides a direct link between developmental regulators, components of the cell-cycle machinery and organ patterning.
- R. Sozzani
- , H. Cui
- & P. N. Benfey
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Article |
Generation of a novel wing colour pattern by the Wingless morphogen
Here, the generation and evolution of the complex spotted wing pattern of Drosophila guttifera are investigated. The findings show that wing spots are induced by the Wingless morphogen, and that the elaborate spot pattern evolved from simpler schemes by co-option of Wingless expression at new sites. This type of process is likely to occur in other animals, too.
- Thomas Werner
- , Shigeyuki Koshikawa
- & Sean B. Carroll
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Letter |
Changes in Hox genes’ structure and function during the evolution of the squamate body plan
The organization of Hox clusters in several different reptiles is investigated, showing that the Hox clusters in squamates — lizards and snakes — have unexpectedly accumulated transposable elements, reflecting extensive genomic rearrangements of coding and non coding regulatory regions. Comparative expression analyses between two species showing different axial skeletons, the corn snake and the whiptail lizard, revealed major alterations in Hox13 and Hox10 expression features during snake somitogenesis, in line with the expansion of both caudal and thoracic regions.
- Nicolas Di-Poï
- , Juan I. Montoya-Burgos
- & Denis Duboule
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Letter |
Rere controls retinoic acid signalling and somite bilateral symmetry
The vertebrate body plan shows marked bilateral symmetry, although this can be disrupted in conditions such as scoliosis. Here, a mutation in Rere is found that leads to the formation of asymmetrical somites in mouse embryos; furthermore, Rere is shown to control retinoic acid signalling, which is required to maintain somite symmetry by interacting with Fgf8. The results provide insight into how bilateral symmetry is maintained.
- Gonçalo C. Vilhais-Neto
- , Mitsuji Maruhashi
- & Olivier Pourquié