Focus

Focus on Next-Generation Sequencing

Focus issue:
October 2008 Volume 26, No 10

This focus summarizes the current state of the art in massively parallel sequencing technologies and how these platforms are changing the face of biological and biomedical research and of the sequencing business itself. With financial support from Roche, the focus will be freely available online from October 2008 to April 2009.

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Editorial

Next-generation sequencing

Prepare for the deluge p1099

doi:10.1038/nbt1008-1099

The gobs of data produced by next-generation sequencing are a key problem limiting wider adoption.

Abstract | Full Text | PDF (132 KB)

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News

Next-generation sequencing

Profile: Rade Drmanac p1100

Laura DeFrancesco

doi:10.1038/nbt1008-1100

Launching the world's first commercial human genome sequencing center is currently the 'unreasonable' career ambition of sequencing-by-hybridization pioneer Rade Drmanac.

Abstract | Full Text | PDF (110 KB)

News Features

Next-generation sequencing

Fixing the front end pp1101 - 1104

Ken Garber

doi:10.1038/nbt1008-1101

One bottleneck in next-generation sequencing is genomic sample selection. As research groups tackle the problem, companies are seizing a market opportunity. Ken Garber reports.

Abstract | Full Text | PDF (163 KB)

Next-generation sequencing

What price personal genome exploration? pp1105 - 1108

Jeffrey L Fox

doi:10.1038/nbt1008-1105

Companies offering direct-to-consumer genomic information face tough questions about who regulates them, where they fit in health care and how to value their services. What will it take to move them from niche services to a broader customer base? Jeffrey Fox reports.

Abstract | Full Text | PDF (390 KB)

Next-generation sequencing

The sequencing shakeup pp1109 - 1112

Amy Coombs

doi:10.1038/nbt1008-1109

Deep sequencing technology could soon be competitive with certain array applications. But the jury remains out on which of the myriad platforms will have the greatest impact and broadest application. Amy Coombs investigates.

Abstract | Full Text | PDF (170 KB)

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Opinion and Comment

Commentary

Next-generation sequencing

How to get genomes at one ten-thousandth the cost pp1113 - 1115

Jeffery A Schloss

doi:10.1038/nbt1008-1113

The NHGRI's Advanced DNA Sequencing Technology program is spearheading the development of platforms that will bring routine whole-genome sequencing closer to reality.

Abstract | Full Text

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Research

Perspectives

Next-generation sequencing

The development and impact of 454 sequencing pp1117 - 1124

Jonathan M Rothberg & John H Leamon

doi:10.1038/nbt1485

Abstract | Full Text | PDF (583 KB)

Next-generation sequencing

What would you do if you could sequence everything? pp1125 - 1133

Avak Kahvejian, John Quackenbush & John F Thompson

doi:10.1038/nbt1494

Abstract | Full Text | PDF (908 KB)

Reviews

Next-generation sequencing

Next-generation DNA sequencing pp1135 - 1145

Jay Shendure & Hanlee Ji

doi:10.1038/nbt1486

Abstract | Full Text | PDF (694 KB)

Next-generation sequencing

The potential and challenges of nanopore sequencing pp1146 - 1153

Daniel Branton, David W Deamer, Andre Marziali, Hagan Bayley, Steven A Benner, Thomas Butler, Massimiliano Di Ventra, Slaven Garaj, Andrew Hibbs, Xiaohua Huang, Stevan B Jovanovich, Predrag S Krstic, Stuart Lindsay, Xinsheng Sean Ling, Carlos H Mastrangelo, Amit Meller, John S Oliver, Yuriy V Pershin, J Michael Ramsey, Robert Riehn, Gautam V Soni, Vincent Tabard-Cossa, Meni Wanunu, Matthew Wiggin & Jeffery A Schloss

doi:10.1038/nbt.1495

Abstract | Full Text | PDF (1,791 KB)

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