Cryoelectron microscopy articles within Nature

Featured

  • Article |

    We propose a model for a sequential, multistep activation mechanism of metabotropic glutamate receptor subtype 5, including a series of structures in lipid nanodiscs, from inactive to fully active, with agonist-bound intermediate states.

    • Kaavya Krishna Kumar
    • , Haoqing Wang
    •  & Brian K. Kobilka

  • Article |

    The molecular basis of OSCA/TMEM63 channel mechanosensitivity was investigated by determining 44 cryogenic electron microscopy structures of channels in different environments, expanding understanding of channel-mediated mechanotransduction and pore formation, with implications for two protein families.

    • Yaoyao Han
    • , Zijing Zhou
    •  & Yixiao Zhang

  • Article
    | Open Access

    Cryo-electron microscopy structures of the human Integrator complex in three different functional states shed light on how Integrator terminates RNA polymerase II (Pol II) transcription by disengaging Pol II from the DNA template.

    • Isaac Fianu
    • , Moritz Ochmann
    •  & Patrick Cramer

  • Article |

    Cryo-electron microscopy and molecular dynamics studies of a Vitiosangium gasdermin pore reveal insights into the assembly of this large and diverse family of membrane pore-forming proteins.

    • Alex G. Johnson
    • , Megan L. Mayer
    •  & Philip J. Kranzusch

  • Article
    | Open Access

    Cryo-electron microscopy structures of human RAD51 in complex with the nucleosome show that RAD51 can adopt two conformations—rings and filaments—and reveal how RAD51 binds to the nucleosome through its N-terminal lobe domain.

    • Takuro Shioi
    • , Suguru Hatazawa
    •  & Hitoshi Kurumizaka

  • Article |

    Time-resolved cryo-EM is used to capture structural transitions during G-protein activation stimulated by a G-protein-coupled receptor.

    • Makaía M. Papasergi-Scott
    • , Guillermo Pérez-Hernández
    •  & Georgios Skiniotis

  • Article
    | Open Access

    A study reports structures of an insect taste receptor in the absence and presence of different sugars, providing details on the molecular basis of sugar detection and selectivity in insects.

    • João Victor Gomes
    • , Shivinder Singh-Bhagania
    •  & Joel A. Butterwick

  • Article |

    Structures of the yeast replisome associated with the FACT complex and an evicted histone hexamer offer insights into the mechanism of replication-coupled histone recycling for maintaining epigenetic inheritance.

    • Ningning Li
    • , Yuan Gao
    •  & Yuanliang Zhai

  • Article
    | Open Access

    Studies using genetic screening, biophysical characterization and structural reconstitution elucidate the mechanism of action and enable rational design of a new class of functional compounds that glue target proteins to E3 ligases via intramolecularly bridging two domains to enhance intrinsic protein–protein interactions and promote target ubiquitination and degradation.

    • Oliver Hsia
    • , Matthias Hinterndorfer
    •  & Alessio Ciulli

  • Article
    | Open Access

    A study identifies a new bacterial ribosome hibernation factor, Balon, and describes its association with EF-Tu and its initiation of mRNA-independent hibernation during protein synthesis.

    • Karla Helena-Bueno
    • , Mariia Yu. Rybak
    •  & Sergey V. Melnikov

  • Article |

    The cryo-electron microscopy structure of the human phagocyte NADPH oxidase in the activated state provides insight into how cytosolic factors bind to and promote the activating conformational changes of NOX2, facilitating its efficient electron transfer.

    • Xiaoyu Liu
    • , Yiting Shi
    •  & Lei Chen

  • Article
    | Open Access

    Cryo-electron microscopy structures of intermediates formed during the degradation of the 30S ribosomal unit shed light on how the 3′ to 5′ exonuclease ribonuclease R controls the ribosomal degradation process.

    • Lyudmila Dimitrova-Paternoga
    • , Sergo Kasvandik
    •  & Helge Paternoga

  • Article
    | Open Access

    A mechanism of lipid transport inhibition has been identified for a class of peptide antibiotics effective against resistant Acinetobacter strains, which may have applications in the inhibition of other Gram-negative pathogens.

    • Karanbir S. Pahil
    • , Morgan S. A. Gilman
    •  & Daniel Kahne

  • Article
    | Open Access

    Structural studies show how the PfRCR complex of Plasmodium falciparum forms a bridge between erythrocyte and parasite membranes, and how PfCyRPA-binding antibodies neutralize invasion through a steric mechanism, opening the way to new approaches in rational vaccine design.

    • Brendan Farrell
    • , Nawsad Alam
    •  & Matthew K. Higgins

  • Article
    | Open Access

    X-ray crystallography, cryo-electron microscopy, structural modelling, biochemistry, cell biology, and evolutionary analysis enable characterization of ORF2p, the reverse transcriptase of the ancient ‘parasitic’ LINE-1 retrotransposon that has written around one-third of the human genome.

    • Eric T. Baldwin
    • , Trevor van Eeuwen
    •  & Martin S. Taylor

  • Article
    | Open Access

    Human LINE-1 ORF2p relies on upstream single-stranded target DNA to position the adjacent duplex in the endonuclease active site for nicking of the longer DNA strand, with a single nick generating a staggered DNA break.

    • Akanksha Thawani
    • , Alfredo Jose Florez Ariza
    •  & Kathleen Collins

  • Article |

    Structures of human vesicular monoamine transporter 2 in complexes with serotonin and three clinical drugs provide insights into the structural basis for serotonin transport and inhibition of transporter activity by the drugs.

    • Di Wu
    • , Qihao Chen
    •  & Daohua Jiang

  • Article
    | Open Access

    Cryogenic electron microscopy structures of amyloid filaments extracted from patient brains reveal that the protein TAF15 forms filaments that characterize certain cases of frontotemporal lobar degeneration.

    • Stephan Tetter
    • , Diana Arseni
    •  & Benjamin Ryskeldi-Falcon

  • Article |

    The accuracy of eukaryotic ribosome translocation relies on eukaryote-specific elements of the 80S ribosome, elongation factor 2 and transfer RNAs, all of which contribute to the maintenance of the messenger RNA reading frame.

    • Nemanja Milicevic
    • , Lasse Jenner
    •  & Gulnara Yusupova

  • Article
    | Open Access

    A time-resolved cryogenic electron microscopy analysis provides structural information on the processes of primary and secondary nucleation of tau amyloid formation, with implications for the development of new therapies.

    • Sofia Lövestam
    • , David Li
    •  & Sjors H. W. Scheres

  • Article
    | Open Access

    Single-particle cryo-electron microscopy structures of HIV-1 envelope (Env) trimers bound to one or two CD4 receptors identify intermediate Env conformations that precede host–virus fusion and inform the design of therapeutics to prevent HIV-1 infection.

    • Kim-Marie A. Dam
    • , Chengcheng Fan
    •  & Pamela J. Bjorkman

  • Article |

    The intricate molecular architecture and interactions of the human cardiac myosin filament offer insights into cardiac physiology, disease and drug therapy.

    • Debabrata Dutta
    • , Vu Nguyen
    •  & Roger Craig

  • Article
    | Open Access

    Upon hyperpolarization, the S4 voltage-sensing segment of sea urchin SLC9C1 moves down, removing inhibition caused by an intracellular helix and enabling Na+/H+ exchange, leading to pH-dependent activation of sAC and sperm chemotaxis.

    • Hyunku Yeo
    • , Ved Mehta
    •  & David Drew

  • Article
    | Open Access

    Binding of a sialoglycan-based primary receptor by the spike protein of the common cold human coronavirus HKU1 triggers conformational changes to a state that would allow binding to a second receptor required for cell entry.

    • Matti F. Pronker
    • , Robert Creutznacher
    •  & Daniel L. Hurdiss

  • Review Article |

    Structural studies of amyloid filaments purified from brains of people with neurodegenerative diseases link specific amyloid folds with distinct diseases and provide a basis for the development of models of neurodegenerative disease.

    • Sjors H. W. Scheres
    • , Benjamin Ryskeldi-Falcon
    •  & Michel Goedert

  • Article
    | Open Access

    The exploration of voltage-gated potassium channels using cryo-electron microscopy and electrophysiology identifies a mechanism of inactivation involved in regulating neuron firing.

    • Ana I. Fernández-Mariño
    • , Xiao-Feng Tan
    •  & Kenton J. Swartz

  • Article |

    High-speed atomic force microscopy single-molecule imaging and cryo-EM analysis discover and reveal the structure of a TRPV3 pentamer, providing evidence for a non-canonical pentameric TRP-channel assembly, laying the foundation for new directions in TRP channel research.

    • Shifra Lansky
    • , John Michael Betancourt
    •  & Simon Scheuring

  • Article |

    Cryo-electron microscopy structures of OPA1, mutations of which are associated with the disease dominant optic atrophy, provide insight into how structural features of OPA1 enable this protein to mediate mitochondrial-membrane fusion and remodelling.

    • Sarah B. Nyenhuis
    • , Xufeng Wu
    •  & Jenny E. Hinshaw

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