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Cellulose is a burgeoning feedstock for more sustainable alternatives to fossil fuel-based materials. This protocol uses a cellulose-dissolving ionic liquid electrolyte with nuclear magnetic resonance methods to provide high-resolution structural analysis of cellulose.
The authors describe spiral volumetric optoacoustic tomography for the visualization of contrast agent perfusion and biodistribution in mice, featuring excellent scalability to achieve spatial resolution down to 90 µm or whole-body scans in <2 s.
We present the design, assembly and implementation of a molecular-electromechanical system composed of a tetrahedral DNA nanostructure immobilized on a graphene field-effect transistor for unamplified detection of trace amounts of analytes in biofluids.
We present a protocol for the encapsulation and stabilization of enzymes by using hydrogen-bonded organic frameworks (HOFs)—enzyme@HOF-101—along with procedures for their material characterization and testing of their biocatalytic performance.
This protocol enables the generation of human forebrain organoids from induced pluripotent stem cells with the intrinsic ability to assemble functionally integrated bilateral optic vesicles.
AbEMap generates large ensembles of docked antigen–antibody structures based on the structure of an antigen and either the structure or the sequence of an antibody. For each antigen residue, a likelihood score for being part of the epitope is obtained.
A protocol for the directed differentiation of human pluripotent stem cells into distal lung organoids that can be used to model interstitial lung disease, viral infection and human lung development, along with procedures for their analysis.
This Perspective explains how click chemistry—specifically, the copper-catalyzed azide–alkyne cycloaddition, the strain-promoted azide–alkyne cycloaddition and the inverse electron-demand Diels–Alder reaction—has revolutionized radiopharmaceutical chemistry.
This CRISPR-Combo system enables efficient multiplexed orthogonal genome editing and transcriptional activation in plants. Here, the use of CRISPR-Combo is demonstrated for speed breeding of transgene-free, genome-edited Arabidopsis and enhancing rice regeneration with more germline mutations.
This protocol for Multiplexed Intermixed CRISPR Droplets uses microfluidics to create droplets containing Cas9, multiplexed single-guide RNAs and corresponding DNA barcodes, allowing large-scale genetic screens to be performed in F0 zebrafish.
This protocol describes the use of Retro-Cascorder to make temporally resolved transcriptional recordings in Escherichia coli DNA, using a retron reverse transcriptase to store transcriptional events in a unidirectionally expanding CRISPR array via acquisition by CRISPR–Cas integrases.
The authors present a surgical approach to isolate neural cells from macaques with fast and efficient steps, combined with optimized culturing conditions, to improve the yield and quality of cell populations for downstream analyses
Graphene-based single-molecule junctions, integrated with an electrical circuit, facilitate the detection of electronic, optical and mechanical properties of reactions at the molecular scale.
This Protocol Extension describes Zebrafish targeting of Reactive Electrophiles and oXidants (Z-REX), an extension of a Protocol on the targetable reactive electrophiles and oxidants, adapted for on-demand redox targeting in live zebrafish embryos. The protocol details how to generate transgenic fish and run Z-REX, as well as its downstream validation.
Micro Capture-C is a chromatin conformation capture method for visualizing reproducible three-dimensional contacts of regulatory regions in the genome at base-pair resolution.
This Protocol Extension describes an operant model in which mice lever press to obtain rewarding social interaction with a peer. The model can be used to study the role of operant social reward and other neuropsychiatric disorders with a component of social dysfunction.
This protocol isolates large and small extracellular vesicles, as well as nonvesicular nanoparticles known as exomeres and supermeres, from cell-conditioned medium or human plasma via differential ultracentrifugation, filtration, concentration and high-resolution density-gradient fractionation.