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Volume 19 Issue 3, March 2024

Studying human brain vascular cells with single-cell transcriptomics and organoids

Human brain vascular cells, including endothelial and mural cells, can be purified with FACS and used in many downstream applications, including cell culture, transcriptomics and organoid transplants. This image shows vascular cells labeled with GFP and transplanted on top of induced pluripotent stem cell (iPSC)-derived cortical organoids. See Crouch et al. p603

Image : Elizabeth Crouch, University of California San Francisco. Cover design: S. Whitham

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  • This article describes the main sources of bias in small RNA-sequencing and the importance of obtaining structure-level information for the correct interpretation of high-throughput sequencing results.

    • Juan Pablo Tosar
    • Mauricio Castellano
    • Alfonso Cayota
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