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This article describes the main sources of bias in small RNA-sequencing and the importance of obtaining structure-level information for the correct interpretation of high-throughput sequencing results.
This Perspective covers recent advances in mass spectrometry and explains how these approaches may be used to study the structure and function of protein condensates undergoing liquid–liquid phase separation.
This Tutorial provides guide for how to evaluate, select and use publicly available computational tools for predicting intrinsic disorder in proteins, with a focus on performance and ease of use results, exemplified using results from the Critical Assessment of protein Intrinsic Disorder prediction experiment.
We provide a Perspective highlighting the significant ethical implications of the use of fast-developing neurotechnologies in humans, as well as the regulatory frameworks and guidelines needed to protect neurodata and mental privacy.
This tutorial provides guidelines for imputing human leukocyte antigen alleles, including standard quality control measures for input genotyping data and best practice recommendations for association testing and fine-mapping to identify causal alleles.
This tutorial outlines common strategies for analysis of bulk RNA-sequencing (RNA-seq) data in the context of tumor immunity and immunotherapy response and presents a comprehensive computational pipeline integrating these tools to streamline RNA-seq analysis.
This Perspective explains how click chemistry—specifically, the copper-catalyzed azide–alkyne cycloaddition, the strain-promoted azide–alkyne cycloaddition and the inverse electron-demand Diels–Alder reaction—has revolutionized radiopharmaceutical chemistry.
In this Perspective, authors from the UK Consortium on Metabolic Phenotyping propose a ‘fit-for-purpose’, four-tiered framework to evaluate the reliability of targeted metabolomics analyses, addressing the need for community-accepted, harmonized guidelines for tiers other than full validation.
In this Perspective, the authors propose genetically engineered mouse models to further the understanding and use of a recently discovered tool, selective endogenous encapsidation for cellular delivery via virus-like particles, in gene delivery and gene therapy applications.
Transcranial electrical stimulation (tES) can be used in combination with functional MRI (fMRI) to study brain functions. This Consensus Statement provides a checklist for reporting concurrent tES-fMRI research.
Cue reactivity measured by functional magnetic resonance imaging is used in studies of substance-use disorders. This Consensus Statement is the result of a Delphi process to arrive at parameters that should be reported in describing these studies.
This review explores factors to consider when introducing electronic laboratory notebooks, including discussion of integration with research data management and the functionalities to compare when evaluating specific software packages.
This Perspective discusses the use of CRISPR–Cas9-mediated gene conversion systems in laboratory and wild rodents. Although technical hurdles must be overcome before wild-release strategies are feasible, modest improvements could increase the efficiency and reduce the cost of mouse genetics in the laboratory.
The authors describe three-dimensional imaging pipelines available to analyze archival patient specimens. The pipelines facilitate the visualization of both large and small volumes of tissue with subcellular resolution.
This tutorial describes how to develop a nanoinjection workflow, including the selection of nanoneedle devices, approaches to loading cargo, strategies for interfacing to biological systems and assays to evaluate the efficacy of intracellular delivery.
Mass-spectrometry-based proteomics is a powerful approach for discovering disease biomarkers. This tutorial provides advice on the study design, including cohort selection, evaluating statistical power, blinding and randomization, and quality control.
Understanding the heterogeneity of extracellular vesicles is crucial for unraveling their functions. This review describes the benefits, challenges and contributions of the state-of-the art methods used in single-vesicle analysis.
This tutorial provides guidelines for interpreting single-cell transcriptomic maps to identify cell types, states and other biologically relevant patterns.
This tutorial provides guidance for selecting and optimizing tissue-clearing protocols for specific samples and biological questions. In addition, instructions are provided for developing an imaging strategy and processing the resulting data.