Microscopy

Microscopy refers to any method used to acquire images of nearby objects at resolutions that greatly exceed the resolving ability of the unaided human eye. Object visualization may be mediated by light or electron beams using optical or magnetic lenses respectively, or through the use of a physical scanning probe that measures one of a wide range of different sample characteristics.

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Latest Research and Reviews

  • Research | | open

    During stress, protein synthesis is inhibited through phosphorylation of the initiation factor eIF2 on its alpha subunit and its interaction with eIF2B. Here the authors describe a structure of the yeast eIF2B in complex with its substrate - the GDP-bound phosphorylated eIF2, providing insights into how phosphorylation results in a tighter interaction with eIF2B.

    • Yuliya Gordiyenko
    • , José Luis Llácer
    •  & V. Ramakrishnan
  • Research | | open

    Epsilon toxin (Etx) is a potent pore forming toxin (PFT) produced by Clostridium perfringens. Here authors show the cryo-EM structure of the Etx pore assembled on the membrane of susceptible cells and shed light on pore formation and mutant phenotypes.

    • Christos G. Savva
    • , Alice R. Clark
    • , Claire E. Naylor
    • , Michel R. Popoff
    • , David S. Moss
    • , Ajit K. Basak
    • , Richard W. Titball
    •  & Monika Bokori-Brown
  • Research | | open

    Ribonucleotide reductase (RNR) catalyzes the conversion of ribonucleotides to deoxyribonucleotides, which is an essential step in DNA synthesis. Here the authors use small-angle X-ray scattering, X-ray crystallography, and cryo-electron microscopy to capture active and inactive forms of the Bacillus subtilis RNR and provide mechanistic insights into a convergent form of allosteric regulation.

    • William C. Thomas
    • , F. Phil Brooks III
    • , Audrey A. Burnim
    • , John-Paul Bacik
    • , JoAnne Stubbe
    • , Jason T. Kaelber
    • , James Z. Chen
    •  & Nozomi Ando
  • Research | | open

    Radiation induced sample deformation can be a limiting factor for X-ray imaging resolution at the nanoscale. The authors report a tomographic model that estimates and accounts for morphological changes during data acquisition and enables reconstruction of a high-resolution image ab initio.

    • Michal Odstrcil
    • , Mirko Holler
    • , Jörg Raabe
    • , Alessandro Sepe
    • , Xiaoyuan Sheng
    • , Silvia Vignolini
    • , Christian G. Schroer
    •  & Manuel Guizar-Sicairos
  • Research | | open

    Translation termination is under strong selection pressure for high speed and accuracy. Here the authors provide a 3D view of the dynamics of a translating bacterial ribosome as it recruits a class-1 release factor (RF1 or RF2) upon encountering a stop codon, and propose a structure-based kinetic model for the early steps in bacterial translation termination.

    • Ziao Fu
    • , Gabriele Indrisiunaite
    • , Sandip Kaledhonkar
    • , Binita Shah
    • , Ming Sun
    • , Bo Chen
    • , Robert A. Grassucci
    • , Måns Ehrenberg
    •  & Joachim Frank

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