Volume 18 Issue 9, September 2023

This protocol for universal and proficient Pseudomonas recombineering uses phage-encoded homologous recombination–Cas3 systems, including SacB counterselection and Cre site-specific recombinase for two- or three-step seamless genome modification.

This protocol is for using CapQuant, a mass spectrometry-based technique for accurate and sensitive quantification of the epitranscriptome of RNA caps using stable isotope-labeled cap nucleotides.

The authors provide a detailed protocol for the isolation of four membrane protein complexes (transmembrane channel-like proteins 1 and 2, lipid transfer protein and ‘Protein S’) from transgenic C. elegans.

Combining surface-enhanced Raman spectroscopy with surface-accessible nanomaterials allows molecule–metal interactions to be probed in the complex environments that are directly relevant to their application. This will underpin the development of improved functional nanomaterials.

This protocol describes how to measure the rate of racemization in atropisomers by (i) kinetic analysis of the racemization of an enantioenriched sample, (ii) dynamic HPLC and (iii) variable-temperature NMR.

A protocol for setting up, calibrating and validating a semi-automated sample processing pipeline for cell-free RNA isolation from clinical samples using the Opentrons 1.0 or 2.0 open-source robotic platform.

The authors provide a protocol for cytosine base editing to introduce precise substitutions into the genome of zebrafish, an important model for genetic studies and in vivo disease modeling.

In this protocol, layered semiconductor crystals undergo electrochemical intercalation of organic cations followed by exfoliation to form 2D nanosheets in solution. These nanosheets can assemble into thin films for diverse electronic applications.

Protocol for the fabrication of a microfluidic device and its implementation in three-dimensional human neural cultures, allowing the study of neuron–glia interactions in neuroinflammation and neurodegeneration.