Volume 18 Issue 10, October 2023

A protocol for broad-spectrum, in vivo fluorescent labeling and tracking of extracellular matrix proteins through the systemic or local application of N-hydroxysuccinimide esters. This flexible approach can be adapted for a variety of organ systems and wounding models.

This protocol describes the critical concepts, experimental standardization, operational principles and data analysis for using on-chip electrocatalytic microdevices.

This Protocol documents the extensive preparation required to adopt Neuropixels probes for use in patients for recording spiking activity from up to hundreds of cortical neurons and associating it with local field potentials.

We present a protocol for generating mouse ESC-derived induced neuronal cells via lentiviral Tet-On inducible expression of NGN2 for the direct comparison of human and mouse neuronal populations.

A simple and robust approach for the large-scale assembly of DNA-templated precise carbon nanotube semiconductor arrays with clean channel interfaces to build field-effect transistors for high-performance electronics.

Purifying proteins by crystallization rather than chromatography is less expensive and more environmentally sustainable. This protocol describes how to determine the operational conditions for purifying monoclonal antibodies by template-assisted membrane crystallization.

A correlative imaging approach in which matrix-assisted laser desorption ionization mass spectrometry imaging is followed by fluorescence in situ hybridization (metaFISH) for the spatial metabolomics identification of host–microbiome interactions, at single-cell resolution, via nucleic acid probes.

Immobilization of enzymes in solid porous supports can enhance the recyclability and stability of enzymes. This protocol describes direct-immobilization methods to make enzyme-covalent organic framework biocomposites.

This protocol describes how to prepare ordered mesoporous intermetallic nanomaterials with controlled compositions, morphologies/structures and phases via a general concurrent template strategy.