Volume 18 Issue 8, August 2023

This tutorial outlines common strategies for analysis of bulk RNA-sequencing (RNA-seq) data in the context of tumor immunity and immunotherapy response and presents a comprehensive computational pipeline integrating these tools to streamline RNA-seq analysis.

This Protocol Extension combines optogenetics with juxtacellular recordings and describes procedures for targeting, recording and labeling individual genetically defined neurons in freely moving mice.

A protocol for the construction of acoustic tweezers to manipulate single cells in a high-throughput, precise, selective and contact-free manner, which can be broadly adapted for investigations across the materials, physical and life sciences.

A general approach, via solution-based self-assembly, for the preparation of two-dimensional mesoporous conducting polymer nanosheets with tunable pore size and thickness, and defined morphology and composition.

A protocol for differentiation of human pluripotent stem cells into three-dimensional ureteric bud organoids that exhibit complex morphological development and the capacity to differentiate into functional collecting duct tissues.

This protocol describes how to coat polymeric nanoparticles (e.g., bare poly (lactic-co-glycolic acid) (50:50, acid terminated) Resomer 504H nanoparticles) with ionic liquids to improve their circulation half-life and biodistribution after intravenous injection. This is achieved via red blood cell hitchhiking in whole blood.

Laser-based sampling techniques are commonly used in many mass spectrometry imaging approaches. With this protocol, subcellular spatial resolution is achieved by focusing the laser with an optical fiber with an end rounded to form a microlens.

A protocol for fabricating quantum mechanical tunneling probes via carbon and gold deposition, functionalized by surface modifications such as biotinylated thiols, that enable conductance to be measured in single proteins in solution for up to several hours.

Understanding how proteins and materials interact is useful for evaluating their safety and function. This protocol describes a dimethyl labeling strategy for determining the protein-material interface and discovering allosteric structural changes.