Articles

  • Article |

    Nanoscale vibrations provided by a bioreactor induce the differentiation of mesenchymal stem cells into mineralized tissue in three dimensions, independently of other environmental factors.

    • Penelope M. Tsimbouri
    • , Peter G. Childs
    • , Gabriel D. Pemberton
    • , Jingli Yang
    • , Vineetha Jayawarna
    • , Wich Orapiriyakul
    • , Karl Burgess
    • , Cristina González-García
    • , Gavin Blackburn
    • , Dilip Thomas
    • , Catalina Vallejo-Giraldo
    • , Manus J. P Biggs
    • , Adam S. G. Curtis
    • , Manuel Salmerón-Sánchez
    • , Stuart Reid
    •  & Matthew J. Dalby
  • Article |

    Ultrasound pulses controlling the release of an anaesthetic encapsulated in liposomes allow for the timing, intensity and duration of sciatic-nerve blocks in rats.

    • Alina Y. Rwei
    • , Juan L. Paris
    • , Bruce Wang
    • , Weiping Wang
    • , Christopher D. Axon
    • , María Vallet-Regí
    • , Robert Langer
    •  & Daniel S. Kohane
  • Article |

    A system consisting of a glass microelectrode and a boron-doped diamond microsensor can simultaneously track, in rat brains and in the guinea pig cochlea, the local real-time kinetics of injected drugs and the resulting electrophysiological activity.

    • Genki Ogata
    • , Yuya Ishii
    • , Kai Asai
    • , Yamato Sano
    • , Fumiaki Nin
    • , Takamasa Yoshida
    • , Taiga Higuchi
    • , Seishiro Sawamura
    • , Takeru Ota
    • , Karin Hori
    • , Kazuya Maeda
    • , Shizuo Komune
    • , Katsumi Doi
    • , Madoka Takai
    • , Ian Findlay
    • , Hiroyuki Kusuhara
    • , Yasuaki Einaga
    •  & Hiroshi Hibino
  • Article |

    Uniform iron oxide nanoparticle clusters are highly biocompatible and can be used as contrast agents for high-resolution magnetic resonance angiography of large animals.

    • Yang Lu
    • , Yun-Jun Xu
    • , Guo-bing Zhang
    • , Daishun Ling
    • , Ming-quan Wang
    • , Yong Zhou
    • , Ya-Dong Wu
    • , Tao Wu
    • , Michael J. Hackett
    • , Byung Hyo Kim
    • , Hogeun Chang
    • , Jonghoon Kim
    • , Xin-Tian Hu
    • , Liang Dong
    • , Nohyun Lee
    • , Fangyuan Li
    • , Jia-Cai He
    • , Li Zhang
    • , Hui-Qin Wen
    • , Bo Yang
    • , Seung Hong Choi
    • , Taeghwan Hyeon
    •  & Duo-Hong Zou
  • Article |

    Polymer–lipid–peptide nanoparticles carrying an antiplatelet antibody and a chemotherapy drug deplete tumour-associated platelets to increase vascular permeability and augment the accumulation of the drug in tumours.

    • Suping Li
    • , Yinlong Zhang
    • , Jing Wang
    • , Ying Zhao
    • , Tianjiao Ji
    • , Xiao Zhao
    • , Yanping Ding
    • , Xiaozheng Zhao
    • , Ruifang Zhao
    • , Feng Li
    • , Xiao Yang
    • , Shaoli Liu
    • , Zhaofei Liu
    • , Jianhao Lai
    • , Andrew K. Whittaker
    • , Gregory J. Anderson
    • , Jingyan Wei
    •  & Guangjun Nie
  • Article |

    An array-based method can identify synthetic hydrogels that outperform Matrigel in screening for vascular-disruptor compounds and in supporting the expansion and pluripotency of human embryonic stem cells.

    • Eric H. Nguyen
    • , William T. Daly
    • , Ngoc Nhi T. Le
    • , Mitra Farnoodian
    • , David G. Belair
    • , Michael P. Schwartz
    • , Connie S. Lebakken
    • , Gene E. Ananiev
    • , Mohammad Ali Saghiri
    • , Thomas B. Knudsen
    • , Nader Sheibani
    •  & William L. Murphy
  • Article |

    The biophysical properties of cells can be used to predict the cellular age of humans with higher accuracy than conventional biomolecular markers.

    • Jude M. Phillip
    • , Pei-Hsun Wu
    • , Daniele M. Gilkes
    • , Wadsworth Williams
    • , Shaun McGovern
    • , Jena Daya
    • , Jonathan Chen
    • , Ivie Aifuwa
    • , Jerry S. H. Lee
    • , Rong Fan
    • , Jeremy Walston
    •  & Denis Wirtz
  • Article |

    Large microscale arrays of zymosan particle clusters enable the characterization of human-neutrophil swarming, including the presence of start and stop signals, and the deficient swarming behaviour of neutrophils from patients following major trauma.

    • Eduardo Reátegui
    • , Fatemeh Jalali
    • , Aimal H. Khankhel
    • , Elisabeth Wong
    • , Hansang Cho
    • , Jarone Lee
    • , Charles N. Serhan
    • , Jesmond Dalli
    • , Hunter Elliott
    •  & Daniel Irimia
  • Article |

    A library of single-strand oligonucleotide probes with a common long-adapter sequence can clone, in a single reaction, thousands of open reading frames spanning 400–5,000 bp, and has been applied to DNA from human stool and microbiome samples.

    • Lorenzo Tosi
    • , Viswanadham Sridhara
    • , Yunlong Yang
    • , Dongli Guan
    • , Polina Shpilker
    • , Nicola Segata
    • , H. Benjamin Larman
    •  & Biju Parekkadan
  • Article |

    A light-sheet microscope images large surgical and biopsy specimens non-destructively over large fields of view in two and three dimensions, with the same level of detail as traditional slide-based histopathology.

    • Adam K. Glaser
    • , Nicholas P. Reder
    • , Ye Chen
    • , Erin F. McCarty
    • , Chengbo Yin
    • , Linpeng Wei
    • , Yu Wang
    • , Lawrence D. True
    •  & Jonathan T. C. Liu
  • Article |

    A comparison of the in vivo engraftment of scaffolds containing either an unassembled suspension of human vascular cells or an assembled network of them shows that non-inflammatory host neutrophils are indispensable mediators of vascularization.

    • Ruei-Zeng Lin
    • , Chin Nien Lee
    • , Rafael Moreno-Luna
    • , Joseph Neumeyer
    • , Breanna Piekarski
    • , Pingzhu Zhou
    • , Marsha A. Moses
    • , Monisha Sachdev
    • , William T. Pu
    • , Sitaram Emani
    •  & Juan M. Melero-Martin
  • Article |

    In a rabbit model of prosthetic joint infection, optimization of the shape and loading of antibiotic clusters in a polymer implant augments and prolongs antibiotic elution while maintaining implant strength and wear rate.

    • V. J. Suhardi
    • , D. A. Bichara
    • , S. J. J. Kwok
    • , A. A. Freiberg
    • , H. Rubash
    • , H. Malchau
    • , S. H. Yun
    • , O. K. Muratoglu
    •  & E. Oral
  • Article |

    3D-printed grafts with geometrically designed and endothelialized vasculature rescue the perfusion of distal tissues in rodent models of hind limb ischaemia and myocardial infarction, preventing capillary loss, muscle atrophy and loss of function.

    • T. Mirabella
    • , J. W. MacArthur
    • , D. Cheng
    • , C. K. Ozaki
    • , Y. J. Woo
    • , M. T. Yang
    •  & C. S. Chen
  • Article |

    A simple and versatile assay that relies on the bioconjugation capabilities and ultrafast and localized deposition of polydopamine can be plugged into common laboratory bioassays to improve their detection sensitivity by orders of magnitude.

    • Junwei Li
    • , Madison A. Baird
    • , Michael A. Davis
    • , Wanyi Tai
    • , Larry S. Zweifel
    • , Kristina M. Adams Waldorf
    • , Michael Gale Jr
    • , Lakshmi Rajagopal
    • , Robert H. Pierce
    •  & Xiaohu Gao
  • Article |

    An optimized formulation of glucagon-like peptide-1 recombinantly fused to an elastin-like polypeptide leads to zero-order release kinetics from a subcutaneous depot and to 10 days of glycaemic control in three mouse models of diabetes.

    • Kelli M. Luginbuhl
    • , Jeffrey L. Schaal
    • , Bret Umstead
    • , Eric M. Mastria
    • , Xinghai Li
    • , Samagya Banskota
    • , Susan Arnold
    • , Mark Feinglos
    • , David D’Alessio
    •  & Ashutosh Chilkoti
  • Article |

    Single-impulse photoacoustic computed tomography can, at deep penetration and high resolution and contrast, image the whole-body dynamics of small animals in real time, and track injected cancer cells and image the vasculature of whole rat brains.

    • Lei Li
    • , Liren Zhu
    • , Cheng Ma
    • , Li Lin
    • , Junjie Yao
    • , Lidai Wang
    • , Konstantin Maslov
    • , Ruiying Zhang
    • , Wanyi Chen
    • , Junhui Shi
    •  & Lihong V. Wang
  • Article |

    A closed-loop control system measures and adjusts the concentration of a chemotherapeutic in real time and maintains it within a predefined therapeutic window in both rabbits and rats.

    • P. L. Mage
    • , B. S. Ferguson
    • , D. Maliniak
    • , K. L Ploense
    • , T. E. Kippin
    •  & H. T. Soh
  • Article |

    An efficient and chemically defined protocol for the differentiation of human induced pluripotent stem cells into podocytes enables the recapitulation of the differential clearance of the human kidney glomerulus in an organ-on-a-chip.

    • Samira Musah
    • , Akiko Mammoto
    • , Thomas C. Ferrante
    • , Sauveur S. F. Jeanty
    • , Mariko Hirano-Kobayashi
    • , Tadanori Mammoto
    • , Kristen Roberts
    • , Seyoon Chung
    • , Richard Novak
    • , Miles Ingram
    • , Tohid Fatanat-Didar
    • , Sandeep Koshy
    • , James C. Weaver
    • , George M. Church
    •  & Donald E. Ingber
  • Article |

    Nanoscale extracellular vesicles can be efficiently isolated in about 15 minutes, for downstream analyses of nucleic acids and proteins, via spontaneous labelling through a lipid nanoprobe and subsequent magnetic capture of the labelled vesicles.

    • Yuan Wan
    • , Gong Cheng
    • , Xin Liu
    • , Si-Jie Hao
    • , Merisa Nisic
    • , Chuan-Dong Zhu
    • , Yi-Qiu Xia
    • , Wen-Qing Li
    • , Zhi-Gang Wang
    • , Wen-Long Zhang
    • , Shawn J. Rice
    • , Aswathy Sebastian
    • , Istvan Albert
    • , Chandra P. Belani
    •  & Si-Yang Zheng