Microscopy articles within Nature Photonics

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  • Article
    | Open Access

    Electrochemical control of the switching of fluorophores in stochastic optical reconstruction microscopy (EC-STORM) enables the counting of single fluorophores as well as cell imaging with improved spatial resolution and reduced artefacts compared with traditional STORM.

    • Ying Yang
    • , Yuanqing Ma
    •  & J. Justin Gooding

  • Article |

    Wide-field mid-infrared photothermal imaging is developed to supress the resolution degradation caused by photo-thermal heat diffusion. By employing a single-objective synthetic-aperture imaging with synchronized subnanosecond mid-infrared and visible light sources, spatial resolution of 120 nm is obtained.

    • Miu Tamamitsu
    • , Keiichiro Toda
    •  & Takuro Ideguchi

  • News & Views |

    A non-common-path interferometric scheme enables holographic detection of single proteins of mass 90 kDa and estimation of single-protein polarizability.

    • Chia-Lung Hsieh

  • Article
    | Open Access

    Time-resolved lightwave-driven scanning tunnelling spectroscopy is developed to investigate how the spin–orbit-split energy levels of a selenium vacancy within a WSe2 monolayer shift under phonon displacement. Ultrafast snapshots of the electronic tunnelling spectra reveal transient energy shifts up to 40 meV.

    • C. Roelcke
    • , L. Z. Kastner
    •  & Y. A. Gerasimenko

  • Article
    | Open Access

    Holographic microscopy with independent control of the signal and reference fields enables the holographic imaging of a single protein with mass below 100 kDa and estimation of their polarizability.

    • Jan Christoph Thiele
    • , Emanuel Pfitzner
    •  & Philipp Kukura

  • News & Views |

    Brillouin light scattering anisotropy microscopy affords single-shot collection of angle-resolved phonon dispersion, enabling the mapping of mechanical anisotropies in living matter with a frequency resolution of 10 MHz and a spatial resolution of 2 µm.

    • Yogeshwari S. Ambekar
    •  & Giuliano Scarcelli

  • Article |

    Super-resolution pMINFLUX microscopy is combined with FRET and enables co-tracking of two fluorophores without photoswitching.

    • Fiona Cole
    • , Jonas Zähringer
    •  & Philip Tinnefeld

  • Review Article |

    This Review covers a comparison between various label-free biomedical imaging techniques, their advantages over label-based methods and relevant applications.

    • Natan T. Shaked
    • , Stephen A. Boppart
    •  & Jürgen Popp

  • News & Views |

    The introduction of a two-step deconvolution workflow maximizes the detection of fluorescence in fluctuation-based super-resolution imaging, enabling a square millimetre field of view to be captured in as little as ten minutes.

    • David Baddeley

  • Article
    | Open Access

    Spatial-frequency tracking adaptive beacon light-field encoded endoscopy enables imaging through a single multimode fibre under bending and twisting. In vivo imaging with subcellular resolution is demonstrated in mice models.

    • Zhong Wen
    • , Zhenyu Dong
    •  & Qing Yang

  • News & Views |

    Two papers in Science demonstrate tracking of the stepping motion of the kinesin motor protein with nanometric spatial precision and sub-millisecond temporal resolution by using MINFLUX, a highly photon-efficient single-molecule localization technique.

    • Fernando D. Stefani

  • News & Views |

    A photothermal microscopy technique overcomes the diffraction limit by exploiting the spatiotemporal dynamics of heat dissipation within the imaging volume, offering new opportunities for super-resolution, bond-selective and label-free imaging of biological targets.

    • Zhilun Zhao
    •  & Wei Min

  • News & Views |

    Ultrasound-induced gas bubbles in tissue can temporarily minimize optical scattering, enabling laser light to be focused at greater depth for higher-resolution imaging.

    • Paul Beard
    •  & Kishan Dholakia

  • Article |

    Nitrogen-vacancy centres in surface-engineered diamond are demonstrated to operate as charge-sensitive fluorescent reporters, enabling an optical scheme for voltage recording in physical and biological systems.

    • D. J. McCloskey
    • , N. Dontschuk
    •  & D. A. Simpson

  • Obituary |

    Gabriel Popescu passed away in June 2022. He will be remembered as a creative leader in biophotonics, with pioneering contributions to quantitative phase imaging and spectroscopy, an engaging collaborator and a dear friend.

    • Natan T. Shaked
    • , YongKeun Park
    •  & Peter T. C. So

  • Article |

    Researchers exploit Rayleigh waves and associated dynamic strains to control exciton transport in the weak coupling regime at room temperature. The findings may pave the way for new types of excitonic device for applications ranging from communications to energy.

    • Kanak Datta
    • , Zhengyang Lyu
    •  & Parag B. Deotare

  • Review Article |

    This Review summarizes the latest state-of-the-art technologies for high-speed multiphoton (fluorescence) microscopy, especially at kilohertz 2D frame rate, and 3D video rate or beyond—a speed regime that was generally inconceivable until very recently, as well as the prospects and challenges of these emerging technologies.

    • Jianglai Wu
    • , Na Ji
    •  & Kevin K. Tsia

  • Article
    | Open Access

    A stimulated-emission-depletion-based fluorescence localization and super-resolution microscopy concept that is capable of attaining a spatial resolution down at the size scale of the fluorophores themselves and a localization precision of 1–3 nm in standard deviation is reported.

    • Michael Weber
    • , Marcel Leutenegger
    •  & Stefan W. Hell

  • Article |

    Adapting the amplitude-modulated light detection and ranging approach to super-resolution microscopy offers a typical axial localization precision of 6.8 nm over the entire field of view and the axial capture range, enabling imaging of biological samples by up to several micrometres in depth.

    • Pierre Jouchet
    • , Clément Cabriel
    •  & Sandrine Lévêque-Fort

  • News & Views |

    Directly relating the complex second-harmonic-generation field to the second-order susceptibility tensor allows tomographic imaging of nonlinear optical contrast at high frame rates.

    • Paul J. Campagnola

  • Review Article |

    Recent improvements of the operation speed of variable optical elements are reviewed with an emphasis on components with microsecond focus-varying response time.

    • SeungYeon Kang
    • , Martí Duocastella
    •  & Craig B. Arnold

  • News & Views |

    Using a photonic chip to generate the patterns of light needed for structured illumination microscopy could reduce the cost and complexity of super-resolution imaging.

    • Sara Abrahamsson

  • Article |

    A tomographiac approach to second-harmonic-generation imaging on nonlinear structures is demonstrated, with experiments and three-dimensional reconstructions on a beta-barium borate crystal and various biological specimens performed.

    • Chenfei Hu
    • , Jeffrey J. Field
    •  & Gabriel Popescu

  • Article |

    The use of a photonic integrated circuit to both hold a biological sample and generate the necessary light patterns for structured illumination microscopy promises convenient super-resolution imaging.

    • Øystein Ivar Helle
    • , Firehun Tsige Dullo
    •  & Balpreet Singh Ahluwalia

  • News & Views |

    Dark-field microscopy is a widely used imaging method that emphasizes sharp edges and other small features, but typically requires specialized microscope components. Researchers have now engineered special substrates that enable dark-field microscopy using simple bright-field microscopes.

    • Mikhail A. Kats

  • Article |

    Transscleral optical phase imaging, which is based on transscleral flood illumination of the retina, is demonstrated to provide cellular-resolution, label-free, high-contrast images of the retinal layers over a large field of view without the drawback of a long exposure time.

    • Timothé Laforest
    • , Mathieu Künzi
    •  & Christophe Moser

  • Article |

    Vertical integration of a metalens to realize compound nanophotonic systems for optical analog image processing is realized, significantly reducing the size and complexity of conventional optical systems.

    • You Zhou
    • , Hanyu Zheng
    •  & Jason Valentine

  • News & Views |

    Quantitative phase gradient images can now be captured in a single shot thanks to the use of two layers of compact, multifunctional dielectric metasurfaces.

    • YoonSeok Baek
    •  & YongKeun Park

  • News & Views |

    Using graphene as the ‘metal’ layer improves the localization accuracy of metal-induced energy transfer by nearly tenfold.

    • Margarida M. Barroso

  • News & Views |

    A wide-field system that can perform video-rate imaging of the entire area of the brain of an awake mouse is aiding the study of neurones, epilepsy and the immune system.

    • Gail McConnell

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