Biological techniques articles within Nature Photonics

Featured

  • Article
    | Open Access

    Electrochemical control of the switching of fluorophores in stochastic optical reconstruction microscopy (EC-STORM) enables the counting of single fluorophores as well as cell imaging with improved spatial resolution and reduced artefacts compared with traditional STORM.

    • Ying Yang
    • , Yuanqing Ma
    •  & J. Justin Gooding

  • Article |

    Wide-field mid-infrared photothermal imaging is developed to supress the resolution degradation caused by photo-thermal heat diffusion. By employing a single-objective synthetic-aperture imaging with synchronized subnanosecond mid-infrared and visible light sources, spatial resolution of 120 nm is obtained.

    • Miu Tamamitsu
    • , Keiichiro Toda
    •  & Takuro Ideguchi

  • News & Views |

    Ultrasound-induced luminescence in trianthracene derivative-based nanoparticles enables tumour imaging and immunological profiling in a variety of in vivo models.

    • Cheng Xu
    •  & Kanyi Pu

  • News & Views |

    A non-common-path interferometric scheme enables holographic detection of single proteins of mass 90 kDa and estimation of single-protein polarizability.

    • Chia-Lung Hsieh

  • News & Views |

    Brillouin light scattering anisotropy microscopy affords single-shot collection of angle-resolved phonon dispersion, enabling the mapping of mechanical anisotropies in living matter with a frequency resolution of 10 MHz and a spatial resolution of 2 µm.

    • Yogeshwari S. Ambekar
    •  & Giuliano Scarcelli

  • Article |

    Super-resolution pMINFLUX microscopy is combined with FRET and enables co-tracking of two fluorophores without photoswitching.

    • Fiona Cole
    • , Jonas Zähringer
    •  & Philip Tinnefeld

  • Review Article |

    This Review covers a comparison between various label-free biomedical imaging techniques, their advantages over label-based methods and relevant applications.

    • Natan T. Shaked
    • , Stephen A. Boppart
    •  & Jürgen Popp

  • News & Views |

    Event-based detectors, which respond to local changes in light intensity rather than producing images, enable super-resolution single-molecule localization microscopy with sensitivity and resolution comparable to conventional methods.

    • Ian M. Dobbie

  • News & Views |

    The introduction of a two-step deconvolution workflow maximizes the detection of fluorescence in fluctuation-based super-resolution imaging, enabling a square millimetre field of view to be captured in as little as ten minutes.

    • David Baddeley

  • Article
    | Open Access

    Spatial-frequency tracking adaptive beacon light-field encoded endoscopy enables imaging through a single multimode fibre under bending and twisting. In vivo imaging with subcellular resolution is demonstrated in mice models.

    • Zhong Wen
    • , Zhenyu Dong
    •  & Qing Yang

  • Article |

    Two-photon excitation with mid- and near-infrared pulses encodes bond selectivity in fluorescence imaging. Single-molecule imaging and spectroscopy is demonstrated on individual fluorophores as well as various labelled biological targets.

    • Haomin Wang
    • , Dongkwan Lee
    •  & Lu Wei

  • News & Views |

    Ultrasound-induced gas bubbles in tissue can temporarily minimize optical scattering, enabling laser light to be focused at greater depth for higher-resolution imaging.

    • Paul Beard
    •  & Kishan Dholakia

  • News & Views |

    The demonstration that diamond nitrogen–vacancy centre technology can optically detect voltages with an impressive sensitivity could bring new opportunities for investigating neurobiology.

    • Milos Nesladek
    •  & Micha E. Spira

  • Obituary |

    Gabriel Popescu passed away in June 2022. He will be remembered as a creative leader in biophotonics, with pioneering contributions to quantitative phase imaging and spectroscopy, an engaging collaborator and a dear friend.

    • Natan T. Shaked
    • , YongKeun Park
    •  & Peter T. C. So

  • News & Views |

    The chiral nature of phonons in crystals of biomolecules is identified by terahertz spectroscopy, paving the way to a better understanding of biochemical processes.

    • Minkyu Kim
    •  & Vladimir V. Tsukruk

  • Article |

    Chiral phonons—long-range lattice vibrations with rotational motion of atoms—are observed by terahertz chiroptical spectroscopy in biocrystals. Terahertz circular dichroism peaks between 0.2 and 2.0 THz clearly identify the chirality of these phonons in various microcrystalline and nanofibrils of biomolecules.

    • Won Jin Choi
    • , Keiichi Yano
    •  & Nicholas A. Kotov

  • News & Views |

    Measurement of the arrival times of annihilation photons in a detector with greater precision is opening the way to new direct forms of tomographic positron emission imaging that do not require back-projection-based reconstruction techniques.

    • Suleman Surti
    •  & Joel S. Karp

  • Review Article |

    This Review summarizes the latest state-of-the-art technologies for high-speed multiphoton (fluorescence) microscopy, especially at kilohertz 2D frame rate, and 3D video rate or beyond—a speed regime that was generally inconceivable until very recently, as well as the prospects and challenges of these emerging technologies.

    • Jianglai Wu
    • , Na Ji
    •  & Kevin K. Tsia

  • Article |

    Positron emission imaging without tomographic reconstruction is demonstrated. A Cherenkov radiation detector detects gamma rays produced by positron–electron annihilation. The position of a positron source is determined with a precision of 4.8 mm.

    • Sun Il Kwon
    • , Ryosuke Ota
    •  & Simon R. Cherry

  • Obituary |

    Radiation pressure exerted by light was a lifelong passion for Arthur Ashkin. He foresaw that light pressure could do useful work and invented the optical tweezers that can trap microscopic objects, from small ‘living things’ down to individual atoms.

    • René-Jean Essiambre

  • News & Views |

    A correlation method that combines ultrasound and fluorescence enables imaging in strongly scattering environments.

    • Allard P. Mosk

  • Article |

    A tomographiac approach to second-harmonic-generation imaging on nonlinear structures is demonstrated, with experiments and three-dimensional reconstructions on a beta-barium borate crystal and various biological specimens performed.

    • Chenfei Hu
    • , Jeffrey J. Field
    •  & Gabriel Popescu

  • News & Views |

    Advanced computational imaging techniques have the potential to extract neural activity patterns from scattered data without reconstructing images.

    • Gordon Wetzstein
    •  & Isaac Kauvar

  • Article |

    The use of a photonic integrated circuit to both hold a biological sample and generate the necessary light patterns for structured illumination microscopy promises convenient super-resolution imaging.

    • Øystein Ivar Helle
    • , Firehun Tsige Dullo
    •  & Balpreet Singh Ahluwalia

  • News & Views |

    Dark-field microscopy is a widely used imaging method that emphasizes sharp edges and other small features, but typically requires specialized microscope components. Researchers have now engineered special substrates that enable dark-field microscopy using simple bright-field microscopes.

    • Mikhail A. Kats

  • News & Views |

    A wide-field system that can perform video-rate imaging of the entire area of the brain of an awake mouse is aiding the study of neurones, epilepsy and the immune system.

    • Gail McConnell

  • Article |

    By synthesizing undistorted cross-sectional image reconstructions from multiple conventional images acquired with angular diversity, optical coherence refraction tomography offers greater than threefold improvement in lateral resolution and speckle reduction in imaging tissue ultrastructure, and reconstructs the tissue’s internal refractive index distribution.

    • Kevin C. Zhou
    • , Ruobing Qian
    •  & Joseph A. Izatt

  • News & Views |

    High-efficiency, time-domain, near-infrared fluorophores provide multiplexed colour channels for distinct deep bioimaging.

    • Shoujun Zhu
    •  & Xiaoyuan Chen

  • News & Views |

    Emerging data reveal that amyloid fibrils possess intrinsic photonic activity, showing luminescence over a wide range of the electromagnetic spectrum from the ultraviolet to the near-infrared.

    • Per Hammarström

Browse broader subjects

Browse narrower subjects

Browse Biological techniques across other nature.com journals