Microscopy

Definition

Microscopy refers to any method used to acquire images of nearby objects at resolutions that greatly exceed the resolving ability of the unaided human eye. Object visualization may be mediated by light or electron beams using optical or magnetic lenses respectively, or through the use of a physical scanning probe that measures one of a wide range of different sample characteristics.

Featured

Latest Research and Reviews

  • Research |

    The structure of human ABCG2 bound to an inhibitory antibody using cryo-electron microscopy, representing the first high-resolution structural data of a human multidrug transporter.

    • Nicholas M. I. Taylor
    • , Ioannis Manolaridis
    • , Scott M. Jackson
    • , Julia Kowal
    • , Henning Stahlberg
    •  & Kaspar P. Locher
  • Research |

    FHIRM-TPM is a miniature two-photon microscope capable of imaging fluorescently labeled neurons in the brains of freely behaving mice. It allows for imaging of spines or recording of neural activity with a frame rate up to 40 Hz.

    • Weijian Zong
    • , Runlong Wu
    • , Mingli Li
    • , Yanhui Hu
    • , Yijun Li
    • , Jinghang Li
    • , Hao Rong
    • , Haitao Wu
    • , Yangyang Xu
    • , Yang Lu
    • , Hongbo Jia
    • , Ming Fan
    • , Zhuan Zhou
    • , Yunfeng Zhang
    • , Aimin Wang
    • , Liangyi Chen
    •  & Heping Cheng
  • Research |

    Structural ensembles of the 70S ribosome bound to cognate or near-cognate charged tRNAs in complex with EF-Tu illustrate the crucial role of the nucleotide G530 in decoding of mRNA, and demonstrate that translational fidelity results from direct control of GTPase by the decoding centre.

    • Anna B. Loveland
    • , Gabriel Demo
    • , Nikolaus Grigorieff
    •  & Andrei A. Korostelev
  • Research | | open

    The proteasome regulates several important cellular processes and has been identified as a target for therapeutic interventions. Here the authors map the conformational and energy landscape of the 26S proteasome upon Oprozomib binding and uncover long-range allosteric effects that control the dynamic behaviour of the proteasome.

    • David Haselbach
    • , Jil Schrader
    • , Felix Lambrecht
    • , Fabian Henneberg
    • , Ashwin Chari
    •  & Holger Stark

News and Comment

  • News and Views |

    The antimalarial mefloquine has been used in the clinic for decades, yet its mode of action has remained elusive. Now, a study reports that the enantiomer (+)-mefloquine binds to the cytosolic ribosome of the major malaria parasite Plasmodium falciparum.

    • Jeremy Burrows
  • News and Views |

    Structural analysis of the mycobacterial ESX-5 secretion complex presents an important step towards understanding how the ESX type VII (T7) secretion systems can translocate a multitude of substrates — including virulence factors involved in pathogenesis — across the bacterial cell envelope.

    • Tracy Palmer
  • News and Views |

    G-protein-coupled receptors are biological targets for drug discovery. Developments in cryo-electron microscopy have enabled the solution of the structure of a class B receptor in complex with its signalling protein. Two biologists and a microscopist explain the exciting implications of this work.

    • Ching-Ju Tsai
    • , Joerg Standfuss
    •  & Robert M. Glaeser
  • News and Views |

    Standard rheology tells us how a cell responds to deformation. But ramping up the frequency reveals more about its internal dynamics and morphology, mapping a route to improved drug treatments — and possible insight into the malignancy of cancers.

    • Klaus Kroy
  • News and Views |

    Biological molecules are often imaged by attaching fluorescent labels — but only a few label types can be used at a time. A method that could smash the record for the number of labels that can be used together is now reported. See Letter p.465

    • Charles H. Camp Jr
    •  & Marcus T. Cicerone
    Nature 544, 423–424