Multiphoton microscopy articles within Nature Photonics

Featured

  • Review Article |

    This Review summarizes the latest state-of-the-art technologies for high-speed multiphoton (fluorescence) microscopy, especially at kilohertz 2D frame rate, and 3D video rate or beyond—a speed regime that was generally inconceivable until very recently, as well as the prospects and challenges of these emerging technologies.

    • Jianglai Wu
    • , Na Ji
    •  & Kevin K. Tsia

  • Article |

    A non-invasive scattering compensation method, termed F-SHARP, gives direct access to the phase and amplitude of the electric-field point spread function, enabling fast and high-resolution correction of aberrations and scattering in living tissue.

    • Ioannis N. Papadopoulos
    • , Jean-Sébastien Jouhanneau
    •  & Benjamin Judkewitz

  • Article |

    A single-source multimodal nonlinear optical imaging system has been developed to probe different endogenous biomolecules. Rapid, stain-free imaging of fresh tissue specimens is possible with short turnaround times for disease diagnosis.

    • Haohua Tu
    • , Yuan Liu
    •  & Stephen A. Boppart

  • Article |

    A high-resolution, broadband imaging system based on coherent anti-Stokes Raman spectroscopy performs rapid, chemically specific imaging of biological tissue. It employs three-colour excitation and operates across the entire biological window.

    • Charles H. Camp Jr
    • , Young Jong Lee
    •  & Marcus T. Cicerone

  • Article |

    A dual-wavelength fibre laser source has been developed for stimulated Raman scattering microscopy. It is precisely tunable over the entire high-wavenumber region of Raman spectra, where most stimulated Raman scattering imaging is performed. Imaging speeds of up to 1 frame s−1 with shot-noise-limited sensitivity were achieved.

    • Christian W. Freudiger
    • , Wenlong Yang
    •  & Khanh Q. Kieu

  • Review Article |

    The ability to dynamically image features deep within living organisms, permitting real-time analysis of cellular structure and function, is important for biological science. This Review article discusses multiphoton microscopy capable of such analysis, along with technologies that are pushing the limits of phenomena that can be quantitatively imaged.

    • Erich E. Hoover
    •  & Jeff A. Squier

  • Letter |

    A parallel implementation of multifocal multiphoton modulation microscopy allows simultaneous phosphorescent lifetime and intensity imaging in vivo at speeds 100 times faster than conventional configurations. Three-dimensional imaging of a phosphorescent quenching dye is also presented.

    • Scott S. Howard
    • , Adam Straub
    •  & Chris Xu

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