Super-resolution microscopy articles within Nature Photonics

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  • Article
    | Open Access

    Electrochemical control of the switching of fluorophores in stochastic optical reconstruction microscopy (EC-STORM) enables the counting of single fluorophores as well as cell imaging with improved spatial resolution and reduced artefacts compared with traditional STORM.

    • Ying Yang
    • , Yuanqing Ma
    •  & J. Justin Gooding

  • Article |

    Wide-field mid-infrared photothermal imaging is developed to supress the resolution degradation caused by photo-thermal heat diffusion. By employing a single-objective synthetic-aperture imaging with synchronized subnanosecond mid-infrared and visible light sources, spatial resolution of 120 nm is obtained.

    • Miu Tamamitsu
    • , Keiichiro Toda
    •  & Takuro Ideguchi

  • Article |

    Super-resolution pMINFLUX microscopy is combined with FRET and enables co-tracking of two fluorophores without photoswitching.

    • Fiona Cole
    • , Jonas Zähringer
    •  & Philip Tinnefeld

  • News & Views |

    The introduction of a two-step deconvolution workflow maximizes the detection of fluorescence in fluctuation-based super-resolution imaging, enabling a square millimetre field of view to be captured in as little as ten minutes.

    • David Baddeley

  • Article
    | Open Access

    Spatial-frequency tracking adaptive beacon light-field encoded endoscopy enables imaging through a single multimode fibre under bending and twisting. In vivo imaging with subcellular resolution is demonstrated in mice models.

    • Zhong Wen
    • , Zhenyu Dong
    •  & Qing Yang

  • News & Views |

    Two papers in Science demonstrate tracking of the stepping motion of the kinesin motor protein with nanometric spatial precision and sub-millisecond temporal resolution by using MINFLUX, a highly photon-efficient single-molecule localization technique.

    • Fernando D. Stefani

  • News & Views |

    A photothermal microscopy technique overcomes the diffraction limit by exploiting the spatiotemporal dynamics of heat dissipation within the imaging volume, offering new opportunities for super-resolution, bond-selective and label-free imaging of biological targets.

    • Zhilun Zhao
    •  & Wei Min

  • Article
    | Open Access

    A stimulated-emission-depletion-based fluorescence localization and super-resolution microscopy concept that is capable of attaining a spatial resolution down at the size scale of the fluorophores themselves and a localization precision of 1–3 nm in standard deviation is reported.

    • Michael Weber
    • , Marcel Leutenegger
    •  & Stefan W. Hell

  • Article |

    Adapting the amplitude-modulated light detection and ranging approach to super-resolution microscopy offers a typical axial localization precision of 6.8 nm over the entire field of view and the axial capture range, enabling imaging of biological samples by up to several micrometres in depth.

    • Pierre Jouchet
    • , Clément Cabriel
    •  & Sandrine Lévêque-Fort

  • News & Views |

    Using a photonic chip to generate the patterns of light needed for structured illumination microscopy could reduce the cost and complexity of super-resolution imaging.

    • Sara Abrahamsson

  • Article |

    The use of a photonic integrated circuit to both hold a biological sample and generate the necessary light patterns for structured illumination microscopy promises convenient super-resolution imaging.

    • Øystein Ivar Helle
    • , Firehun Tsige Dullo
    •  & Balpreet Singh Ahluwalia

  • News & Views |

    Using graphene as the ‘metal’ layer improves the localization accuracy of metal-induced energy transfer by nearly tenfold.

    • Margarida M. Barroso

  • Article |

    A two-step sequential broadband nanofocusing technique offers an external efficiency of ~50% over nearly all the visible range on a fibre-coupled plasmonic nanowire probe. Its integration with a scanning tunnelling microscope realizes lens-free tip-enhanced Raman spectroscopy with 1 nm spatial resolution.

    • Sanggon Kim
    • , Ning Yu
    •  & Ruoxue Yan

  • News & Views |

    Quantum correlations from photon antibunching enhance the resolution of image scanning microscopy in biological imaging by twofold, four times beyond the diffraction limit.

    • Andrew Forbes
    •  & Valeria Rodriguez-Fajardo

  • Article |

    Characterizing not only the fluorescence intensity but also the inherent quantum correlations of the fluorescent photon stream can enhance the spatial resolution of image scanning microscopy up to twofold, a fourfold improvement over the diffraction limit.

    • Ron Tenne
    • , Uri Rossman
    •  & Dan Oron

  • News & Views |

    In the quest for nanoscopy with super-resolution, consensus from the imaging community is that super-resolution is not always needed and that scientists should choose an imaging technique based on their specific application.

    • Rachel Won

  • News & Views |

    The science of superoscillations and the creation of local regions of light on a subwavelength scale is attracting attention for new forms of super-resolution microscopy and stiffer optical traps.

    • Oliver Graydon

  • Article |

    Nanoscopy on a chip makes it possible to perform super-resolution imaging of biological specimens with a wide field-of-view.

    • Robin Diekmann
    • , Øystein I. Helle
    •  & Balpreet S. Ahluwalia

  • Article |

    Stimulated emission double depletion addresses the issue of background in super-resolution imaging and quantitative microscopy through implementation of a two-pulse sequence in a modified stimulated emission depletion set-up. The measured background intensity is removed from each voxel in the acquired images thanks to time-resolved detection.

    • Peng Gao
    • , Benedikt Prunsche
    •  & G. Ulrich Nienhaus

  • News & Views |

    A 2016 competition to find the optimum software for 3D single-molecule localization microscopy will help practitioners choose the best tool for the job and spur further developments in the field.

    • Seamus Holden
    •  & Daniel Sage

  • Article |

    By exploiting a second off state of a reversibly switchable fluorophore, a general approach that can reduce photobleaching and enhance resolution of coordinate-targeted fluorescence nanoscopy has been demonstrated.

    • Johann G. Danzl
    • , Sven C. Sidenstein
    •  & Stefan W. Hell

  • Article |

    Researchers exploit direct stochastic optical reconstruction microscopy and dedicated detection of super-critical-angle fluorescence emission to enable direct optical nanoscopy with axially localized detection.

    • N. Bourg
    • , C. Mayet
    •  & S. Lévêque-Fort

  • News & Views |

    The award of this year's Nobel Prize in Chemistry to the pioneers of various optical schemes capable of achieving super-resolution and single-molecule detection is recognition of a revolution in optical imaging.

    • Michel Orrit

  • Commentary |

    Applying structured illumination microscopy to coherent imaging modalities such as scattering does not yield any additional information beyond that provided by oblique illumination. It thus yields no resolution enhancement over the Abbe diffraction limit, which was derived precisely for that case.

    • Kai Wicker
    •  & Rainer Heintzmann

  • Letter |

    A means for localizing fluorescent molecules over distances of hundreds of nanometres exploits the energy transfer between a donor molecule and surface plasmons on a metal film. The technique is demonstrated by using it to profile the membranes of living cells.

    • Alexey I. Chizhik
    • , Jan Rother
    •  & Jörg Enderlein

  • News & Views |

    Sustaining the ongoing revolution in optical microscopy will require gaining detailed insight into the optical fields in focal spots. Researchers have developed an elegant method for mapping the full electric vector field using just a metal nanosphere on a glass substrate.

    • L. Kuipers

  • Letter |

    An easily implementable reconstruction scheme is demonstrated for determining the full vectorial amplitude and relative phase distributions of highly confined electromagnetic fields with subwavelength resolution from a single-scan measurement. This scheme will help improve microscopy and nanoscopy techniques.

    • Thomas Bauer
    • , Sergej Orlov
    •  & Gerd Leuchs

  • News & Views |

    Using a pump–probe technique, scientists have experimentally demonstrated a nonlinear imaging scheme that permits the super-resolution imaging of nonfluorescent samples, making it promising for use with unstained specimens.

    • Rainer Heintzmann

  • Letter |

    A scheme for overcoming the diffraction limit in the far-field imaging of non-fluorescent species is demonstrated. This technique, which is based on the spatially controlled saturation of electronic absorption, may enable the super-resolution imaging of nanomaterials and non-fluorescent chromophores.

    • Pu Wang
    • , Mikhail N. Slipchenko
    •  & Ji-Xin Cheng

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