Special Feature
Method of the Year 2014
- Special Feature
Our Method of the Year 2014 goes to light-sheet fluorescence microscopy. This series of papers discusses how this technology, in combination with increasingly sophisticated cameras and powerful computing, is dramatically changing and enabling the imaging of living biological samples from developing embryos to functioning brains. We also highlight methods worth watching in the upcoming years.
Editorial
Special feature: Method of the Year 2014
Method of the Year 2014 - p1
doi:10.1038/nmeth.3251
Light-sheet fluorescence microscopy can image living samples in three dimensions with relatively low phototoxicity and at high speed.
Abstract - Method of the Year 2014 | Full Text - Method of the Year 2014 | PDF (65 KB) - Method of the Year 2014
News Feature
Special feature: Method of the Year 2014
Pump up the volume - pp19 - 22
Michael Eisenstein
doi:10.1038/nmeth.3220
Light-sheet fluorescence microscopy techniques are enabling researchers to achieve dynamic, long-term imaging and three-dimensional reconstruction of specimens ranging from single cells to whole embryos.
Abstract - Pump up the volume | Full Text - Pump up the volume | PDF (522 KB) - Pump up the volume
Commentary
Special feature: Method of the Year 2014
Light-sheet fluorescence microscopy for quantitative biology - pp23 - 26
Ernst H K Stelzer
doi:10.1038/nmeth.3219
In light sheet–based fluorescence microscopy (LSFM), optical sectioning in the excitation process minimizes fluorophore bleaching and phototoxic effects. Because biological specimens survive long-term three-dimensional imaging at high spatiotemporal resolution, LSFM has become the tool of choice in developmental biology.
Abstract - Light-sheet fluorescence microscopy for quantitative biology | Full Text - Light-sheet fluorescence microscopy for quantitative biology | PDF (438 KB) - Light-sheet fluorescence microscopy for quantitative biology
Special feature: Method of the Year 2014
Light-sheet imaging for systems neuroscience - pp27 - 29
Philipp J Keller, Misha B Ahrens & Jeremy Freeman
doi:10.1038/nmeth.3214
Developments in electrical and optical recording technology are scaling up the size of neuronal populations that can be monitored simultaneously. Light-sheet imaging is rapidly gaining traction as a method for optically interrogating activity in large networks and presents both opportunities and challenges for understanding circuit function.
Abstract - Light-sheet imaging for systems neuroscience | Full Text - Light-sheet imaging for systems neuroscience | PDF (482 KB) - Light-sheet imaging for systems neuroscience
Special feature: Method of the Year 2014
Guide to light-sheet microscopy for adventurous biologists - pp30 - 34
Emmanuel G Reynaud, Jan Peychl, Jan Huisken & Pavel Tomancak
doi:10.1038/nmeth.3222
Ten years of development in light-sheet microscopy have led to spectacular demonstrations of its capabilities. The technology is ready to assist biologists in tackling scientific problems, but are biologists ready for it? Here we discuss the interdisciplinary challenges light-sheet microscopy presents for biologists and highlight available resources.
Abstract - Guide to light-sheet microscopy for adventurous biologists | Full Text - Guide to light-sheet microscopy for adventurous biologists | PDF (840 KB) - Guide to light-sheet microscopy for adventurous biologists
Methods to Watch
Special feature: Method of the Year 2014
DIA mass spectrometry - p35
Allison Doerr
doi:10.1038/nmeth.3234
Data-independent acquisition (DIA) mass spectrometry may change how proteomic data are generated.
Abstract - DIA mass spectrometry | Full Text - DIA mass spectrometry | PDF (662 KB) - DIA mass spectrometry
Special feature: Method of the Year 2014
Understanding noncoding RNAs - p35
Nicole Rusk
doi:10.1038/nmeth.3235
Methods to profile and characterize the function of noncoding RNAs will emerge.
Abstract - Understanding noncoding RNAs | Full Text - Understanding noncoding RNAs | PDF (662 KB) - Understanding noncoding RNAs
Special feature: Method of the Year 2014
In vivo voltage sensors - p36
Nina Vogt
doi:10.1038/nmeth.3236
Genetically encoded voltage indicators are on the brink of allowing neuronal activity to be directly imaged in vivo.
Abstract - In vivo voltage sensors | Full Text - In vivo voltage sensors | PDF (1,248 KB) - In vivo voltage sensors
Special feature: Method of the Year 2014
Next-generation CRISPRs - p36
Nicole Rusk
doi:10.1038/nmeth.3237
As the CRISPR-Cas system matures, specificity, efficacy and maybe even a eukaryotic nuclease are being considered.
Abstract - Next-generation CRISPRs | Full Text - Next-generation CRISPRs | PDF (1,248 KB) - Next-generation CRISPRs
Special feature: Method of the Year 2014
Structures from tiny crystals - p37
Allison Doerr
doi:10.1038/nmeth.3238
Protein structures can be determined from microcrystals using X-ray and electron diffraction.
Abstract - Structures from tiny crystals | Full Text - Structures from tiny crystals | PDF (535 KB) - Structures from tiny crystals
Special feature: Method of the Year 2014
Super-resolution CLEM - p37
Natalie de Souza
doi:10.1038/nmeth.3239
Correlated light and electron microscopy (CLEM) is particularly powerful when applied in super-resolution.
Abstract - Super-resolution CLEM | Full Text - Super-resolution CLEM | PDF (535 KB) - Super-resolution CLEM
Special feature: Method of the Year 2014
Nanopores for proteins - p38
Tal Nawy
doi:10.1038/nmeth.3240
Nanopores hold promise for single-protein characterization.
Abstract - Nanopores for proteins | Full Text - Nanopores for proteins | PDF (550 KB) - Nanopores for proteins
Special feature: Method of the Year 2014
Imaging at depth - p38
Nina Vogt
doi:10.1038/nmeth.3241
A closer look into the depths of organs such as the brain is within reach.
Abstract - Imaging at depth | Full Text - Imaging at depth | PDF (550 KB) - Imaging at depth