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Mid-infrared metabolic imaging with vibrational probes

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Volume 17 Issue 7

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    ZipSeq uses patterned illumination and photocaged oligonucleotides to serially print barcodes onto live, intact tissues for spatially mapping single cell transcriptomes.

    • Kenneth H. Hu
    • , John P. Eichorst
    • , Chris S. McGinnis
    • , David M. Patterson
    • , Eric D. Chow
    • , Kelly Kersten
    • , Stephen C. Jameson
    • , Zev J. Gartner
    • , Arjun A. Rao
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    A review on recent method development for discovering T cell antigens.

    • Alok V. Joglekar
    •  & Guideng Li

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    The Ribosolve pipeline combines single-particle cryo-EM, M2-seq biochemical analysis and Rosetta auto-DRRAFTER modeling to guide three-dimensional RNA structure determination.

    • Kalli Kappel
    • , Kaiming Zhang
    • , Zhaoming Su
    • , Andrew M. Watkins
    • , Wipapat Kladwang
    • , Shanshan Li
    • , Grigore Pintilie
    • , Ved V. Topkar
    • , Ramya Rangan
    • , Ivan N. Zheludev
    • , Joseph D. Yesselman
    • , Wah Chiu
    •  & Rhiju Das

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    PULSE is an optogenetic tool that consists of two modules with different wavelength sensitivities. Their interplay enables optogenetic access to gene expression in plants independently of ambient light.

    • Rocio Ochoa-Fernandez
    • , Nikolaj B. Abel
    • , Franz-Georg Wieland
    • , Jenia Schlegel
    • , Leonie-Alexa Koch
    • , J. Benjamin Miller
    • , Raphael Engesser
    • , Giovanni Giuriani
    • , Simon M. Brandl
    • , Jens Timmer
    • , Wilfried Weber
    • , Thomas Ott
    • , Rüdiger Simon
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  • Brief Communication |

    Hundred-fold-faster DNA-PAINT imaging is enabled by the introduction of concatenated, periodic DNA sequence motifs in the docking strand. Six orthogonal sequences are described for speed-optimized and highly multiplexed cellular imaging.

    • Sebastian Strauss
    •  & Ralf Jungmann

  • Article |

    Small vibrational tags (azide, 13C-edited carbonyl and deuterium-labeled probes) were introduced as metabolic probes for mid-infrared imaging. The tags allow unprecedented in situ visualization of metabolism in cells and animals with high information throughput.

    • Lixue Shi
    • , Xinwen Liu
    • , Lingyan Shi
    • , H. Ted Stinson
    • , Jeremy Rowlette
    • , Lisa J. Kahl
    • , Christopher R. Evans
    • , Chaogu Zheng
    • , Lars E. P. Dietrich
    •  & Wei Min
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    Producing reliable atomic- or close-to-atomic-resolution structures of RNA-only molecules has been a formidable task. Ribosolve can solve sub-nanometer-resolution cryo-EM structures of unbound RNA molecules with unprecedented accuracy and speed.

    • Jane S. Richardson

  • Editorial |

    Systemic racism has held back under-represented minorities, especially Black people, in scientific research. We’re committed in our roles as journal editors to help correct this inequality.

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    In situ labeling with azido sugars enables in vivo immunomodulation of dendritic cells.

    • Madhura Mukhopadhyay
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Expanding the CRISPR Toolbox

Expanding the CRISPR Toolbox

The CRISPR-Cas9 system is best known for its ability to knock out or replace specific genes, via targeted cleavage of the genome. But scientists are developing many more applications, typically by using an inactive Cas9 to target other enzymes to specific genomic sites. From transcriptional regulation to base editing, these developments are extending the range of biological questions that can be probed with CRISPR/Cas9.
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