Special Feature
Five Year Anniversary Special
- Special Feature issue:
- October 2009 Volume 6, No 10
Nature Methods celebrates its five year anniversary with commentaries discussing the impact and progress of methodological developments in the life sciences. We also include a fun selection of papers and covers from our pages.
Editorial
Special Feature: Five Year Anniversary Special
In celebration of methods - p687
doi:10.1038/nmeth1009-687
As evidenced by the cake adorning the cover, Nature Methods is five years old. To celebrate this anniversary, we look at methodological development and its role in scientific inquiry.
Abstract - | Full Text - Five Year Anniversary Special | PDF (72 KB) - Five Year Anniversary Special
Historical Commentaries
Special Feature: Five Year Anniversary Special
Technical matters: method, knowledge and infrastructure in twentieth-century life science- pp701 - 705
Angela N H Creager & Hannah Landecker
doi:10.1038/nmeth1009-701
Conceptual breakthroughs in science tend to garner accolades and attention. But, as the invention of tissue culture and the development of isotopic tracers show, innovative methods open up new fields and enable the solution of longstanding problems.
Abstract - | Full Text - Super-resolution microscopy: breaking the limits | PDF (530 KB) - Super-resolution microscopy: breaking the limits
Special Feature: Five Year Anniversary Special
Seeing things: from microcinematography to live cell imaging- pp707 - 709
Hannah Landecker
doi:10.1038/nmeth1009-707
From histology to microcinematography, from cytochemistry to live cell imaging, the history of visualization technology in the life sciences may be understood as a series of cycles of action and reaction between static and dynamic modes of representing life.
Abstract - | Full Text - Super-resolution microscopy: breaking the limits | PDF (384 KB) - Super-resolution microscopy: breaking the limits
Commentaries
Special Feature: Five Year Anniversary Special
Is sequencing enlightenment ending the dark age of the transcriptome?- pp711 - 713
Piero Carninci
doi:10.1038/nmeth1009-711
Sequencing-based technologies for RNA discovery are playing a key role in deciphering the transcriptome and hold the potential to provide us with a census of RNAs and their functions.
Abstract - | Full Text - Primer: fluorescence imaging under the diffraction limit | PDF (207 KB) - Primer: fluorescence imaging under the diffraction limit
Special Feature: Five Year Anniversary Special
Engineered fluorescent proteins: innovations and applications - pp713 - 717
Michael W Davidson & Robert E Campbell
doi:10.1038/nmeth1009-713
Despite expansion of the fluorescent protein and optical highlighter palette into the orange to far-red range of the visible spectrum, achieving performance equivalent to that of EGFP has continued to elude protein engineers.
Abstract - | Full Text - Putting super-resolution fluorescence microscopy to work | PDF (1,050 KB) - Putting super-resolution fluorescence microscopy to work
Special Feature: Five Year Anniversary Special
Comparative analysis to guide quality improvements in proteomics - pp717 - 719
Matthias Mann
doi:10.1038/nmeth1009-717
The potential of mass spectrometry–based proteomics to advance biology and biomedicine is nearly unlimited but so is its potential for generating bad data. Apart from the pursuit of technological progress in protocols and instruments, stringent comparative analyses of different approaches are critical for fully developing the discipline.
Abstract - | Full Text - Microscopy and its focal switch | PDF (498 KB) - Microscopy and its focal switch
Special Feature: Five Year Anniversary Special
From information to knowledge: new technologies for defining gene function - pp721 - 723
Sean R Collins, Jonathan S Weissman & Nevan J Krogan
doi:10.1038/nmeth1009-721
A wide range of methodology will be needed to bridge the gap between genome sequence and mechanistic understanding in biology. Recent advances in high-throughput genetic screening address this task.
First Paragraph - | Full Text - Induced pluripotency | PDF (420 KB) - Induced pluripotency
Five years of Methods
Special Feature: Five Year Anniversary Special
Five years of Methods- pp724 - 725
doi:10.1038/nmeth1009-724
Abstract - | Full Text - Primer: fluorescence imaging under the diffraction limit | PDF (207 KB) - Primer: fluorescence imaging under the diffraction limit
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