Special Feature

Five Year Anniversary Special

Nature Methods celebrates its five year anniversary with commentaries discussing the impact and progress of methodological developments in the life sciences. We also include a fun selection of papers and covers from our pages.



Special Feature: Five Year Anniversary Special

In celebration of methods p687


As evidenced by the cake adorning the cover, Nature Methods is five years old. To celebrate this anniversary, we look at methodological development and its role in scientific inquiry.


Historical Commentaries

Special Feature: Five Year Anniversary Special

Technical matters: method, knowledge and infrastructure in twentieth-century life sciencepp701 - 705

Angela N H Creager & Hannah Landecker


Conceptual breakthroughs in science tend to garner accolades and attention. But, as the invention of tissue culture and the development of isotopic tracers show, innovative methods open up new fields and enable the solution of longstanding problems.

Special Feature: Five Year Anniversary Special

Seeing things: from microcinematography to live cell imagingpp707 - 709

Hannah Landecker


From histology to microcinematography, from cytochemistry to live cell imaging, the history of visualization technology in the life sciences may be understood as a series of cycles of action and reaction between static and dynamic modes of representing life.



Special Feature: Five Year Anniversary Special

Is sequencing enlightenment ending the dark age of the transcriptome?pp711 - 713

Piero Carninci


Sequencing-based technologies for RNA discovery are playing a key role in deciphering the transcriptome and hold the potential to provide us with a census of RNAs and their functions.

Special Feature: Five Year Anniversary Special

Engineered fluorescent proteins: innovations and applications pp713 - 717

Michael W Davidson & Robert E Campbell


Despite expansion of the fluorescent protein and optical highlighter palette into the orange to far-red range of the visible spectrum, achieving performance equivalent to that of EGFP has continued to elude protein engineers.

Special Feature: Five Year Anniversary Special

Comparative analysis to guide quality improvements in proteomics pp717 - 719

Matthias Mann


The potential of mass spectrometry–based proteomics to advance biology and biomedicine is nearly unlimited but so is its potential for generating bad data. Apart from the pursuit of technological progress in protocols and instruments, stringent comparative analyses of different approaches are critical for fully developing the discipline.

Special Feature: Five Year Anniversary Special

From information to knowledge: new technologies for defining gene function pp721 - 723

Sean R Collins, Jonathan S Weissman & Nevan J Krogan


A wide range of methodology will be needed to bridge the gap between genome sequence and mechanistic understanding in biology. Recent advances in high-throughput genetic screening address this task.


Five years of Methods

Special Feature: Five Year Anniversary Special

Five years of Methods pp724 - 725