Featured
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This Month |
Languages in the lab
Members of a lab often have a varied language background. This rich language diversity leads to lab dynamics that take mindful handling.
- Vivien Marx
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Article
| Open AccessCross-modality supervised image restoration enables nanoscale tracking of synaptic plasticity in living mice
XTC is a supervised deep-learning-based image-restoration approach that is trained with images from different modalities and applied to an in vivo modality with no ground truth. XTC’s capabilities are demonstrated in synapse tracking in the mouse brain.
- Yu Kang T. Xu
- , Austin R. Graves
- & Jeremias Sulam
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Brief Communication
| Open AccessHigh-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics
For EM-based connectomics applications, a staining protocol for large tissue samples in the range of a centimeter has been developed, which avoids artifacts common with established protocols.
- Kun Song
- , Zhihui Feng
- & Moritz Helmstaedter
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Article
| Open AccessGlutamate indicators with improved activation kinetics and localization for imaging synaptic transmission
iGluSnFR variants with improved signal-to-noise ratios and targeting to postsynaptic sites have been developed, enabling the analysis of glutamatergic neurotransmission in vivo as illustrated in the mouse visual and somatosensory cortex.
- Abhi Aggarwal
- , Rui Liu
- & Kaspar Podgorski
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Article
| Open AccessEngineering of NEMO as calcium indicators with large dynamics and high sensitivity
The NEMO series of genetically encoded calcium indicators report calcium activity in neuronal and non-neuronal cells with high signal-to-baseline ratio, which is shown in neuronal culture, slice preparations, in vivo and in planta.
- Jia Li
- , Ziwei Shang
- & Youjun Wang
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Research Briefing |
Photon-based communication between two neurons in Caenorhabditis elegans
Cells exchange information with one another using secreted chemicals as data carriers. We developed an all-optogenetic synaptic transmission system that replaced a chemical neurotransmitter with emitted photons. This system enabled synthetic signaling between unconnected neurons and the generation of prosthetic synaptic circuits.
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Article |
Neural engineering with photons as synaptic transmitters
PhAST is a technology for establishing de novo or modulating synaptic transmission in a light-dependent manner in C. elegans. By combining a calcium-dependent luciferase on pre-synapses with channelrhodopsin on post-synapses, light serves as a synthetic neurotransmitter.
- Montserrat Porta-de-la-Riva
- , Adriana Carolina Gonzalez
- & Michael Krieg
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Article
| Open AccessRapid detection of neurons in widefield calcium imaging datasets after training with synthetic data
DeepWonder removes background signals from widefield calcium recordings and enables accurate and efficient neuronal segmentation with high throughput.
- Yuanlong Zhang
- , Guoxun Zhang
- & Qionghai Dai
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Research Briefing |
Deep-brain photoactivation of an opioid peptide shapes mouse behavior within seconds
Light-activated drugs and signaling molecules have therapeutic potential and are valuable experimental tools. Photoactivation of a mu opioid receptor agonist in the mouse brain rapidly triggered pain relief and locomotion, demonstrating that in vivo photopharmacology can drive dynamic studies into animal behavior.
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Article |
High-speed low-light in vivo two-photon voltage imaging of large neuronal populations
A suite of tools including positive-going voltage indicators, a high-speed two-photon microscope, and denoising software enables prolonged imaging of electrical activity in neurons with limited toxicity.
- Jelena Platisa
- , Xin Ye
- & Jerry L. Chen
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Brief Communication |
In vivo photopharmacology with a caged mu opioid receptor agonist drives rapid changes in behavior
CNV-Y-DAMGO enables photopharmacological manipulations of mu opioid receptor signaling in behaving mice with high temporal resolution.
- Xiang Ma
- , Desiree A. Johnson
- & Matthew R. Banghart
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Brief Communication |
An optical design enabling lightweight and large field-of-view head-mounted microscopes
Two miniature microscopes with innovative light paths are described and applied to imaging of juvenile zebra finches and mice.
- Joseph R. Scherrer
- , Galen F. Lynch
- & Michale S. Fee
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Article |
Femtosecond laser microdissection for isolation of regenerating C. elegans neurons for single-cell RNA sequencing
Femtosecond laser microdissection enables transcriptomic analyses of single neurons based on their phenotype.
- Peisen Zhao
- , Sudip Mondal
- & Adela Ben-Yakar
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News & Views |
Deep brain imaging on the move
New three-photon miniature microscopes open the study of neuronal networks to those deep in the brains of behaving animals.
- Jérôme A. Lecoq
- , Roman Boehringer
- & Benjamin F. Grewe
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Comment |
The end game: respecting major sources of population diversity
Human neuroscience is enjoying burgeoning population data resources: large-scale cohorts with thousands of participant profiles of gene expression, brain scanning and sociodemographic measures. The depth of phenotyping puts us in a better position than ever to fully embrace major sources of population diversity as effects of interest to illuminate mechanisms underlying brain health.
- Jakub Kopal
- , Lucina Q. Uddin
- & Danilo Bzdok
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News & Views |
Linking connectome with transcriptome using a self-inactivating rabies virus
A self-inactivating rabies virus tracer allows long-term monitoring and transcriptomic profiling of synaptically connected cells.
- Shenqin Yao
- & Boaz P. Levi
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Brief Communication |
A modular architecture for organizing, processing and sharing neurophysiology data
A modular architecture for managing and sharing electrophysiology, behavior, colony management and other data has been built to support individual laboratories or large consortia.
- Luigi Acerbi
- , Valeria Aguillon-Rodriguez
- & Miles J. Wells
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Article |
Combining long-term circuit mapping and network transcriptomics with SiR-N2c
A self-inactivating variant of the CVS-N2c rabies virus enables both retrograde viral tracing and transcriptomic analyses, thereby allowing a combination of circuit mapping and molecular studies.
- Hassal Lee
- , Ernesto Ciabatti
- & Marco Tripodi
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Article |
Miniature three-photon microscopy maximized for scattered fluorescence collection
A three-photon miniature microscope with optimized light-collection efficiency facilitates imaging of neuronal activity throughout the cortex, as well as in the hippocampus, in freely moving mice.
- Chunzhu Zhao
- , Shiyuan Chen
- & Heping Cheng
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Article |
Mesoscale volumetric light-field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz
Light-field microscopy is extended to mesoscale fields of view, allowing calcium imaging of thousands of neurons at a high frame rate and high spatial resolution.
- Tobias Nöbauer
- , Yuanlong Zhang
- & Alipasha Vaziri
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Research Highlight |
A cell’s growth rings
Two research groups independently develop tools to record the history of cellular dynamics.
- Hui Hua
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Method to Watch |
Reproducibility in MRI
Strategies to overcome poor reproducibility in MRI studies are needed.
- Nina Vogt
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Research Briefing |
Artificial intelligence gives neuron reconstruction a performance boost
We developed an advanced deep learning approach called local shape descriptors (LSDs) to enable analysis of large electron microscopy datasets with increased efficiency. This technique will speed processing of future petabyte-sized datasets and democratize connectomics research by enabling these analyses using modest computational infrastructure available to most laboratories.
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Resource
| Open AccessMultifaceted atlases of the human brain in its infancy
This Resource presents surface and volume atlases of human brain development during early infancy, at monthly intervals.
- Sahar Ahmad
- , Ye Wu
- & Pew-Thian Yap
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Article
| Open AccessLocal shape descriptors for neuron segmentation
During segmentation of neurons in electron microscopy datasets, auxiliary learning via the prediction of local shape descriptors increases efficiency, which is important for the processing of datasets of ever-increasing size.
- Arlo Sheridan
- , Tri M. Nguyen
- & Jan Funke
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Research Briefing |
Harnessing the multiverse of neuroimaging standard references
We developed a FAIR (findable, accessible, interoperable, reusable) framework for researchers to share spatially standardized brain models. TemplateFlow enables the implementation of more reliable data processing pipelines by maximizing the accessibility of such models. It equips neuroimaging researchers with a foundational tool to bridge gaps between populations and species in neuroscience research.
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Brief Communication |
A large-scale neural network training framework for generalized estimation of single-trial population dynamics
AutoLFADS models neural population activity via a deep learning-based approach with automated hyperparameter optimization.
- Mohammad Reza Keshtkaran
- , Andrew R. Sedler
- & Chethan Pandarinath
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Article
| Open AccessA three-photon head-mounted microscope for imaging all layers of visual cortex in freely moving mice
A lightweight three-photon miniature microscope allows imaging neuronal activity throughout the cortex in freely moving mice.
- Alexandr Klioutchnikov
- , Damian J. Wallace
- & Jason N. D. Kerr
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Technology Feature |
Patch-seq takes neuroscience to a multimodal place
Patch-seq delivers morphology, electrophysiology and transcriptomic data to those with skill, patience and persistence.
- Vivien Marx
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This Month |
Athlete–scientists like to sweat
Some scientists find ways to balance their love of athleticism with research.
- Vivien Marx
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Article |
Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo
cAMPFIREs are genetically encoded cAMP sensors that are suitable for in vivo imaging of cAMP signaling, as demonstrated in Drosophila larvae and behaving mice.
- Crystian I. Massengill
- , Landon Bayless-Edwards
- & Haining Zhong
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News & Views |
Mapping of the zebrafish brain takes shape
The generation of a whole larval zebrafish brain electron microscopy volume in tandem with automated tools lays the groundwork for producing the first vertebrate brain connectome.
- Paul Brooks
- , Andrew Champion
- & Marta Costa
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Resource
| Open AccessAutomated synapse-level reconstruction of neural circuits in the larval zebrafish brain
This Resource presents a serial block-face EM dataset of the whole larval zebrafish brain, including automated segmentation of neurons, detection of synapses and reconstruction of circuitry for visual motion processing.
- Fabian Svara
- , Dominique Förster
- & Herwig Baier
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Brief Communication
| Open AccessSyConn2: dense synaptic connectivity inference for volume electron microscopy
SyConn2 is a machine learning-based framework for inferring and analyzing the connectomes contained in a volume electron microscopy dataset of brain tissue, for example from the zebra finch.
- Philipp J. Schubert
- , Sven Dorkenwald
- & Joergen Kornfeld
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Article
| Open AccessEstimation of skeletal kinematics in freely moving rodents
Pose estimation in combination with an anatomically constrained model allows inferring skeletal kinematics in rodents.
- Arne Monsees
- , Kay-Michael Voit
- & Jason N. D. Kerr
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Article
| Open AccessLight-Seq: light-directed in situ barcoding of biomolecules in fixed cells and tissues for spatially indexed sequencing
Light-Seq uses light-directed DNA barcoding in fixed cells and tissues for multiplexed spatial indexing and subsequent next generation sequencing. This approach blends spatial and omics information to enable analysis of rare cell types in complex tissues.
- Jocelyn Y. Kishi
- , Ninning Liu
- & Peng Yin
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News & Views |
The data science future of neuroscience theory
An approach for integrating the wealth of heterogeneous brain data — from gene expression and neurotransmitter receptor density to structure and function — allows neuroscientists to easily place their data within the broader neuroscientific context.
- Bradley Voytek
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Article
| Open Accessneuromaps: structural and functional interpretation of brain maps
neuromaps is a toolbox for accessing, transforming and comparing human neuroimaging data.
- Ross D. Markello
- , Justine Y. Hansen
- & Bratislav Misic
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Research Briefing |
Lighting up oxytocin dynamics in the brain with MTRIAOT
A genetically encoded green fluorescent sensor for oxytocin, MTRIAOT, offers an opportunity to perform real-time recording of brain oxytocin dynamics in living animals.
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Article
| Open AccessA fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain
A fluorescent sensor for oxytocin called MTRIAOT has been developed. The sensor’s capabilities are demonstrated in fiber photometry measurements in freely behaving mice.
- Daisuke Ino
- , Yudai Tanaka
- & Masaaki Nishiyama
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Article
| Open AccessAntibody stabilization for thermally accelerated deep immunostaining
Thermostable antibodies called SPEARs enable rapid immunostaining with improved tissue penetration.
- Hei Ming Lai
- , Yumi Tang
- & Ho Ko
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Article
| Open AccessFunctional ultrasound localization microscopy reveals brain-wide neurovascular activity on a microscopic scale
Functional ultrasound localization microscopy monitors cerebrovascular blood flow by detecting the flow of injected microbubbles, providing access to brain activity at high spatiotemporal resolution.
- Noémi Renaudin
- , Charlie Demené
- & Mickael Tanter
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Article |
Directed evolution of adeno-associated virus for efficient gene delivery to microglia
Recombinant adeno-associated virus tools for enhanced microglial transduction in mice are reported. These viruses can be used to express functional reporters or genome editing tools with high microglial specificity, with the help of microglia-specific Cre lines.
- Rui Lin
- , Youtong Zhou
- & Minmin Luo
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This Month |
Everyday ups and downs for LGBT+ scientists
How to gauge academic culture and celebrate being an LGBT+ scientist.
- Vivien Marx
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Brief Communication |
ASLPrep: a platform for processing of arterial spin labeled MRI and quantification of regional brain perfusion
ASLPrep is a software suite for reproducible processing of arterial spin labeled magnetic resonance imaging data.
- Azeez Adebimpe
- , Maxwell Bertolero
- & Theodore D. Satterthwaite
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Research Briefing |
NeuroMechFly: an integrative simulation testbed for studying Drosophila behavioral control
Neuromechanical simulations enable the study of how interactions between organisms and their physical surroundings give rise to behavior. NeuroMechFly is an open-source neuromechanical model of adult Drosophila, with data-driven morphological biorealism that enables a synergistic cross-talk between computational and experimental neuroscience.
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- Auditory system
- Blood–brain barrier
- Cell death in the nervous system
- Cellular neuroscience
- Circadian rhythms and sleep
- Cognitive ageing
- Cognitive neuroscience
- Computational neuroscience
- Development of the nervous system
- Diseases of the nervous system
- Emotion
- Epigenetics in the nervous system
- Feeding behaviour
- Genetics of the nervous system
- Glial biology
- Gliogenesis
- Gustatory system
- Ion channels in the nervous system
- Learning and memory
- Molecular neuroscience
- Motivation
- Motor control
- Myelin biology and repair
- Neural ageing
- Neural circuits
- Neuro–vascular interactions
- Neurogenesis
- Neuroimmunology
- Neuronal physiology
- Neurotrophic factors
- Oculomotor system
- Olfactory system
- Peripheral nervous system
- Regeneration and repair in the nervous system
- Reward
- Sensorimotor processing
- Sensory processing
- Sexual behaviour
- Social behaviour
- Social neuroscience
- Somatosensory system
- Spine regulation and structure
- Stem cells in the nervous system
- Stress and resilience
- Synaptic plasticity
- Synaptic transmission
- Transporters in the nervous system
- Visual system