Featured
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Research Briefing |
A method to map single-cell lineages in the mouse brain by CRISPR-based barcoding
We developed CREST (CRISPR editing-based lineage-specific tracing) to enable high-throughput mapping of single-cell lineages in any Cre lineage of interest in mice. In addition, we delineated a comprehensive lineage landscape of the developing mouse ventral midbrain, revealing novel differentiation trajectories and molecular programs underlying neural specification.
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Article |
Comprehensive spatiotemporal mapping of single-cell lineages in developing mouse brain by CRISPR-based barcoding
CREST is a dual-recorder single-cell lineage tracing method for spatiotemporal mapping of the mouse brain.
- Lianshun Xie
- , Hengxin Liu
- & Yuejun Chen
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Research Highlight |
Assembling organs in vitro
Assembloids recapitulate organ spatial organization and interaction with high fidelity.
- Madhura Mukhopadhyay
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Research Highlight |
Recreating the blood–CNS barrier in vitro
Stem-cell-derived organoids reproduce the in vivo secretory and functional properties of the choroid plexus.
- Madhura Mukhopadhyay
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Methods in Brief |
Mapping the spatial proteome of primary neurons
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Research Highlights |
Robots take over electrophysiology
Image-guided targeting of fluorescently labeled neurons enables automated electrophysiological recordings of defined neuronal cell types.
- Nina Vogt
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Tools in Brief |
A switch for manipulating active neurons
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Article |
Fused cerebral organoids model interactions between brain regions
The fusion of patterned cerebral organoids into more complex structures enables modeling of inter-regional processes such as neuronal migration.
- Joshua A Bagley
- , Daniel Reumann
- & Juergen A Knoblich
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Research Highlights |
Cerebral organoids enter the fold
Two groups use cerebral organoids to model brain development and disease.
- Tal Nawy
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Research Highlights |
Manipulating neurons in an activity-dependent manner
Neurons that are activated in response to a particular stimulus or behavior can be labeled or manipulated with a new method.
- Nina Vogt
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Article |
Fixed single-cell transcriptomic characterization of human radial glial diversity
The Fixed and Recovered Intact Single-cell RNA (FRISCR) method enables robust RNA extraction and sequencing from fixed, stained and sorted single cells and allows unprecedented profiling of rare cell types, including two subpopulations of radial glial cells in the developing human cortex.
- Elliot R Thomsen
- , John K Mich
- & Sharad Ramanathan
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Article |
Multispectral labeling technique to map many neighboring axonal projections in the same tissue
The topography of axonal projections can be deciphered by retrograde labeling with multiple overlapping dye injections, as the dye composition in retrogradely transported vesicles is indicative of axonal projection sites.
- Shlomo Tsuriel
- , Sagi Gudes
- & Jeff W Lichtman
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Article |
Fast, accurate reconstruction of cell lineages from large-scale fluorescence microscopy data
This paper describes automated methods for the accurate segmentation and tracking of tens of thousands of nuclei in time-lapse imaging data of developing embryos.
- Fernando Amat
- , William Lemon
- & Philipp J Keller
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Research Highlights |
The developing human brain—modeled in a dish
Stem cells grown in three-dimensional cultures self-organize into tissues that resemble the developing human brain.
- Erika Pastrana
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Research Highlights |
An enhanced view of the brain
A digital atlas of enhancers active in the developing mammalian brain is available for exploration.
- Erika Pastrana
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Research Highlights |
A back door to the neuron
An electrochemical gene-delivery method enables rapid modification of gene expression in postmitotic neurons in vivo, changing their identity and connectivity pattern.
- Erika Pastrana
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Article |
ViBE-Z: a framework for 3D virtual colocalization analysis in zebrafish larval brains
An imaging and registration framework called Virtual Brain Explorer for Zebrafish (ViBE-Z) allows mapping of gene expression patterns and anatomical structures in the zebrafish larval brain. ViBE-Z is provided via a web interface and contains software for image processing, data sets from several developmental stages and a brain atlas.
- Olaf Ronneberger
- , Kun Liu
- & Wolfgang Driever
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Article |
Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy
Simultaneous multiview light-sheet microscopy using two illumination and two detection arms with one- or two-photon illumination is coupled to a fast data acquisition framework and analysis pipeline for quantitative imaging and tracking of individual cells and the developing nervous system throughout a living fly embryo. A related paper by Krzic et al. is also in this issue.
- Raju Tomer
- , Khaled Khairy
- & Philipp J Keller
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Article |
mGRASP enables mapping mammalian synaptic connectivity with light microscopy
In this paper, the authors report GFP reconstitution across synaptic partners (GRASP) adapted for synapse visualization in the mammalian brain.
- Jinhyun Kim
- , Ting Zhao
- & Jeffrey C Magee
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Article |
Real-time multimodal optical control of neurons and muscles in freely behaving Caenorhabditis elegans
An optogenetic illumination system based on the use of a liquid crystal display projector and video tracking software is reported, which allows real-time multispectral light delivery with high spatial resolution to specified targets in freely moving Caenorhabditis elegans. Also in this issue, Leifer et al. report a similar illumination system using a digital micromirror device. Both methods allow optogenetic perturbation of a variety of neural circuits in the behaving worm.
- Jeffrey N Stirman
- , Matthew M Crane
- & Hang Lu
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News Feature |
Light tools
Optogenetics grows from an idea into a discipline. Monya Baker reports.
- Monya Baker
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News & Views |
Watching the fly brain in action
New technical feats make it possible to monitor the activity of identified neurons in awake behaving Drosophila melanogaster.
- Damon A Clark
- , Saskia E J de Vries
- & Thomas R Clandinin
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Article |
Two-photon calcium imaging from head-fixed Drosophila during optomotor walking behavior
This technique allows functional imaging of neurons in head-fixed Drosophila while the fly walks on an air-supported ball. Using a genetically encoded calcium sensor, the activity of motion-sensitive neurons in the fly optic lobe was recorded while the flies were presented with visual stimuli. Activity in these cells correlated with robust optomotor behavior in the walking flies.
- Johannes D Seelig
- , M Eugenia Chiappe
- & Vivek Jayaraman
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Article |
Enhanced neuronal RNAi in C. elegans using SID-1
Expression of the transporter SID-1 in Caenorhabditis elegans neurons renders the cells sensitive to systemic RNAi and permits previously unidentified neuronal phenotypes to be uncovered. This expression also reduces RNAi in nonneuronal cell types, allowing examination of neuronal functions of lethal genes.
- Andrea Calixto
- , Dattananda Chelur
- & Martin Chalfie
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Article |
Computational prediction of neural progenitor cell fates
The fates of cultured neural progenitor cells can be predicted by algorithmic information theory-based computational analysis of time-lapse images of the cells.
- Andrew R Cohen
- , Francisco L A F Gomes
- & Michel Cayouette