Somatosensory system articles within Nature Methods

Featured

• Article | 27 March 2023

  High-speed low-light in vivo two-photon voltage imaging of large neuronal populations

  A suite of tools including positive-going voltage indicators, a high-speed two-photon microscope, and denoising software enables prolonged imaging of electrical activity in neurons with limited toxicity.

  • Jelena Platisa
  • , Xin Ye
  •  & Jerry L. Chen

• Brief Communication | 28 November 2022

  A large-scale neural network training framework for generalized estimation of single-trial population dynamics

  AutoLFADS models neural population activity via a deep learning-based approach with automated hyperparameter optimization.

  • Mohammad Reza Keshtkaran
  • , Andrew R. Sedler
  •  & Chethan Pandarinath

• Brief Communication | 25 November 2019

  A CMOS NMR needle for probing brain physiology with high spatial and temporal resolution

  A miniaturized NMR-on-a-chip needle can be implanted into rodent brains and can measure blood flow and oxygenation changes in vivo in a small volume at an unprecedentedly high temporal resolution of a few milliseconds.

  • Jonas Handwerker
  • , Marlon Pérez-Rodas
  •  & Klaus Scheffler

• Article | 04 November 2019

  Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits

  Simultaneous two-photon microscopic and one-photon mesoscopic imaging of calcium signals enables correlation of local cellular and brain-wide network activity.

  • Daniel Barson
  • , Ali S. Hamodi
  •  & Michael J. Higley

• Brief Communication | 17 June 2019

  Direct wavefront sensing enables functional imaging of infragranular axons and spines

  Two-photon microscopy in combination with adaptive optics enables diffraction-limited morphological and functional imaging up to around 800 μm below the pia. This is achieved with the help of fluorescent microvessels serving as guidestars.

  • Rui Liu
  • , Zengyi Li
  •  & David Kleinfeld

• Article | 14 July 2013

  Functional labeling of neurons and their projections using the synthetic activity–dependent promoter E-SARE

  The synthetic promoter E-SARE provides a genetic tool to tag neurons in an activity-dependent manner. The authors show the utility of this tool for labeling populations of neurons that respond to specific stimuli in living mice and for tracking the axonal projection patterns.

  • Takashi Kawashima
  • , Kazuo Kitamura
  •  & Haruhiko Bito

• Article | 19 February 2012

  Rapid optical control of nociception with an ion-channel photoswitch

  A photoisomerizable molecule, quaternary ammonium–azobenzene–quaternary ammonium (QAQ) enables reversible optical silencing of nociceptive neurons. The selective entry of QAQ into active nociceptive neurons allows spatially and temporally precise regulation of nociceptor activity in vitro and in vivo.

  • Alexandre Mourot
  • , Timm Fehrentz
  •  & Richard H Kramer

• News & Views | 01 June 2010

  Do mice have a pain face?

  Assessing changes in facial expression may enable us to assess pain in animals more accurately and more effectively.

  • Paul A Flecknell

• This Month | 01 June 2010

  Jeffrey Mogil

  Putting a face on pain in mice should improve our ability to measure it.

  • Monya Baker

• Brief Communication | 09 May 2010

  Coding of facial expressions of pain in the laboratory mouse

  The mouse grimace scale offers a standardized behavioral coding system to study the subjective pain experience in mice.

  • Dale J Langford
  • , Andrea L Bailey
  •  & Jeffrey S Mogil