Volume 18 Issue 6, June 2023

This Protocol Extension describes an operant model in which mice lever press to obtain rewarding social interaction with a peer. The model can be used to study the role of operant social reward and other neuropsychiatric disorders with a component of social dysfunction.

Micro Capture-C is a chromatin conformation capture method for visualizing reproducible three-dimensional contacts of regulatory regions in the genome at base-pair resolution.

The authors describe the fabrication, assembly and long-term implantation of ultraflexible neural probes for the simultaneous recording of neural activity and neuromodulation in rodents.

This protocol describes how to use NetColoc, a freely available tool for evaluating the extent to which two related diseases, each characterized by a set of mapped genes, are colocalized in a reference gene interaction network.

This CRISPR-Combo system enables efficient multiplexed orthogonal genome editing and transcriptional activation in plants. Here, the use of CRISPR-Combo is demonstrated for speed breeding of transgene-free, genome-edited Arabidopsis and enhancing rice regeneration with more germline mutations.

The CIRI-long protocol combines circular reverse transcription and long-read nanopore sequencing to capture full-length circular RNA isoforms, allowing their functional characterization.

AbEMap generates large ensembles of docked antigen–antibody structures based on the structure of an antigen and either the structure or the sequence of an antibody. For each antigen residue, a likelihood score for being part of the epitope is obtained.

This protocol for Multiplexed Intermixed CRISPR Droplets uses microfluidics to create droplets containing Cas9, multiplexed single-guide RNAs and corresponding DNA barcodes, allowing large-scale genetic screens to be performed in F0 zebrafish.

This protocol describes the use of Retro-Cascorder to make temporally resolved transcriptional recordings in Escherichia coli DNA, using a retron reverse transcriptase to store transcriptional events in a unidirectionally expanding CRISPR array via acquisition by CRISPR–Cas integrases.

This protocol enables the generation of human forebrain organoids from induced pluripotent stem cells with the intrinsic ability to assemble functionally integrated bilateral optic vesicles.

The authors present a surgical approach to isolate neural cells from macaques with fast and efficient steps, combined with optimized culturing conditions, to improve the yield and quality of cell populations for downstream analyses

Graphene-based single-molecule junctions, integrated with an electrical circuit, facilitate the detection of electronic, optical and mechanical properties of reactions at the molecular scale.