New technologies enable analysis of the extraordinarily diverse and polymorphic components of the immune system–especially the human immune system–at a level of unprecedented detail. Reviews and a Commentary specially commissioned by Nature Biotechnology and Nature Immunology discuss these new methodologies, and how they may be applied to track immune status in health and disease, as well as to reveal new basic immunological insight. A Correspondence describing an initiative to design tools and resources to facilitate these high-dimensional analyses is also included.


New dimensions in immunology


Nature Biotechnology 32, (2014)

Emerging technologies are broadening our understanding of the human immune system, but capitalizing on their application will likely require philosophical and practical changes to the way research is done.



Computational resources for high-dimensional immune analysis from the Human Immunology Project Consortium

Vladimir Brusic, Raphael Gottardo, Steven H Kleinstein, Mark M Davis & HIPC steering committee


Nature Biotechnology 32, (2014)

Brusic, Gottardo and colleagues explain the goals of the Human Immunology Project Consortium.



Quantitative shotgun proteomics: considerations for a high-quality workflow in immunology

Felix Meissner & Matthias Mann


Nature Immunology 15, 112 – 117 (2014)

Proteomics based on high-resolution mass spectrometry has become a powerful tool for the analysis of protein abundance, modifications and interactions. Here we describe technical aspects of proteomics workflows, instrumentation as well as computational considerations to obtain high-quality proteomics data.



Unifying immunology with informatics and multiscale biology

Brian A Kidd, Lauren A Peters, Eric E Schadt & Joel T Dudley


Nature Immunology 15, 118 – 127 (2014)

Dudley and colleagues review some of the computational analysis tools for high-dimensional data and how they can be applied to immunology.

Single-cell technologies for monitoring immune systems

Pratip K Chattopadhyay, Todd M Gierahn, Mario Roederer & J Christopher Love


Nature Immunology 15, 128 – 135 (2014)

Love and colleagues review the limitations of bulk measurements for immune monitoring, and explore advances in single-cell technologies that overcome these problems.

The promise and challenge of high-throughput sequencing of the antibody repertoire

George Georgiou, Gregory C Ippolito, John Beausang, Christian E Busse, Hedda Wardemann & Stephen R Quake


Nature Biotechnology 32, (2014)

Georgiou and colleagues discuss rapidly evolving methods for high-throughput sequencing of the antibody repertoire, and how the resulting data may be applied to answer basic and translational research questions.

Beyond model antigens: high-dimensional methods for the analysis of antigen-specific T cells

Evan W Newell & Mark M Davis


Nature Biotechnology 32, (2014)

Davis and Newell explain how cytometry, and single-cell and sequencing methodologies can be used to characterize human T-cell responses in health and disease.


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