Special feature: Method of the Year 2012

Method of the Year 2012

doi:10.1038/nmeth.2329

New method and tool developments are helping to bring targeted proteome analysis technologies to a broader array of biologists.

Abstract - | Full Text - Method of the Year 2012 | PDF (71 KB) - Method of the Year 2012

Special feature: Method of the Year 2012

Targeted proteomics

Vivien Marx

doi:10.1038/nmeth.2285

Analysis of a preselected group of proteins delivers more precise, quantitative, sensitive data to more biologists. Vivien Marx reports.

Abstract - | Full Text - Targeted proteomics | PDF (1,015 KB)

Special feature: Method of the Year 2012

Mass spectrometry–based targeted proteomics

Allison Doerr

doi:10.1038/nmeth.2286

A brief overview of mass spectrometry technology for targeted proteomics applications is presented.

Abstract - | Full Text - Mass spectrometry–based targeted proteomics | PDF (337 KB)

Special feature: Method of the Year 2012

Proteomics meets the scientific method -

Paola Picotti, Bernd Bodenmiller & Ruedi Aebersold

doi:10.1038/nmeth.2291

By delivering precise, reproducible quantification of proteins of interest in biological samples, targeted proteomics approaches are allowing researchers to apply the scientific method using mass spectrometry.

Abstract - | Full Text - Proteomics meets the scientific method | PDF (197 KB)

Special feature: Method of the Year 2012

Quantitative analysis of peptides and proteins in biomedicine by targeted mass spectrometry -

Michael A Gillette & Steven A Carr

doi:10.1038/nmeth.2309

The role of targeted mass spectrometry technology in the field of clinical proteomics is discussed in this Perspective.

Abstract - | Full Text - Quantitative analysis of peptides and proteins in biomedicine by targeted mass spectrometry | PDF (519 KB)

Special feature: Method of the Year 2012

Disruptive nanopores -

Nicole Rusk

doi:10.1038/nmeth.2292

2013 will see the first commercial nanopore sequencers.

Abstract - | Full Text - Disruptive nanopores | PDF (288KB)

Special feature: Method of the Year 2012

Probing microbiome function -

Tal Nawy

doi:10.1038/nmeth.2293

In studies of microbiome function, marker sequencing will be balanced by alternative profiling approaches.

Abstract - | Full Text - Probing microbiome function | PDF (288 KB)

Special feature: Method of the Year 2012

Near-infrared probes -

Erika Pastrana

doi:10.1038/nmeth.2294

Development of genetically encoded tools with absorption and emission spectra in the near infrared is worth the trouble.

Abstract - | Full Text - Near-infrared probes | PDF (170 KB)

Special feature: Method of the Year 2012

Defective (but useful) diamonds -

Daniel Evanko

doi:10.1038/nmeth.2295

Nitrogen vacancy center defects in diamonds confer remarkably useful properties.

Abstract - | Full Text - Defective (but useful) diamonds | PDF (170 KB)

Special feature: Method of the Year 2012

In vitro niches-

Natalie de Souza

doi:10.1038/nmeth.2296

Attempts to replicate the stem cell microenvironment in the culture dish continue.

Abstract - | Full Text - In vitro niches | PDF (271 KB)

Special feature: Method of the Year 2012

Volumetric imaging in a snapshot -

Erika Pastrana

doi:10.1038/nmeth.2297

Microscopes that render tissue-volume images from single snapshots are making their way into biology.

Abstract - | Full Text - Volumetric imaging in a snapshot | PDF (271 KB)

Special feature: Method of the Year 2012

Mass spectrometry of intact protein complexes -

Allison Doerr

doi:10.1038/nmeth.2298

Mass spectrometry technology to detect and characterize large, intact protein complexes is becoming more accessible.

Abstract - | Full Text - Mass spectrometry of intact protein complexes | PDF (326 KB)

Special feature: Method of the Year 2012

Machines learn phenotypes -

Natalie de Souza

doi:10.1038/nmeth.2299

Automated classifiers speed up biological phenotyping.

Abstract - | Full Text - Machines learn phenotypes | PDF (326 KB)