Imaging articles within Nature Methods

Featured

  • Article |

    Combining reverse transfection of protein tyrosine kinase substrates on cell arrays with fluorescence resonance energy transfer (FRET) measurements by fluorescence lifetime imaging microscopy (FLIM) allows quantitative assessment of phosphorylation patterns and identification of feedback loops at single-cell resolution.

    • Hernán E Grecco
    • , Pedro Roda-Navarro
    •  & Philippe I H Bastiaens

  • Research Highlights |

    Monitoring the activity of neurons in vivo in the freely behaving zebrafish larvae is now possible using bioluminescence, an approach with great potential for unveiling how neuronal networks control behavior.

    • Erika Pastrana

  • Brief Communication |

    Traptavidin, a streptavidin mutant with about tenfold lower 'off' rate for biotin than streptavidin itself, has increased mechanical strength and thermostability. It should find use in a diversity of applications in which the dissociation of streptavidin can be a limitation.

    • Claire E Chivers
    • , Estelle Crozat
    •  & Mark Howarth

  • Research Highlights |

    A new study, pooling brain-imaging data from 35 centers across the world, shows the power of data sharing and demonstrates a universal architecture of functional connections in the human brain.

    • Erika Pastrana

  • Brief Communication |

    A side-view endoscope permits the imaging of large fields of gastrointestinal and respiratory mucosa at high resolution in the mouse. The approach is applied to imaging changes during inflammation and tumor progression in the living mouse.

    • Pilhan Kim
    • , Euiheon Chung
    •  & Seok H Yun

  • News & Views |

    Prospective isolation of defined cell types is a crucial prerequisite for their molecular analysis, but the heterogeneity of populations yielded by current protocols obscures relevant information. New studies now use additional features from time-resolved imaging data for live prospective identification of cells with defined future behavior.

    • Timm Schroeder

  • Article |

    Protein dynamics can be studied in single living cells by time-resolved fluorescent imaging of the unfolding of a fluorescence resonance energy transfer (FRET) probe—labeled protein as fast temperature jumps are applied.

    • Simon Ebbinghaus
    • , Apratim Dhar
    •  & Martin Gruebele

  • Brief Communication |

    By subdividing a charge-coupled device (CCD) array into subgroups using a digital micromirror device and offsetting exposure times, temporal pixel multiplexing allows simultaneous high-speed and high-resolution imaging using a single CCD. This imaging modality allows 250 Hz microscopic imaging of fast cellular responses with a 10-Hz 1.3 megapixel camera

    • Gil Bub
    • , Matthias Tecza
    •  & Peter Kohl

  • Article |

    The fates of cultured neural progenitor cells can be predicted by algorithmic information theory-based computational analysis of time-lapse images of the cells.

    • Andrew R Cohen
    • , Francisco L A F Gomes
    •  & Michel Cayouette

  • Research Highlights |

    Subtle modifications to a red fluorescent protein make it highly effective for intravital imaging.

    • Michael Eisenstein

  • Research Highlights |

    By sampling a two-dimensional diffraction pattern on a spherical detector, three-dimensional structure determination of single molecules should be possible from a single measurement.

    • Allison Doerr

  • Brief Communication |

    Lifetime screening of fluorescent protein variants by fluorescent lifetime imaging microscopy of bacterial colonies identifies bright, high-quantum-yield fluorescent protein variants including a cyan fluorescent protein named mTurquoise that is 1.5-fold brighter than mCerulean and has a mono-exponential fluorescence decay.

    • Joachim Goedhart
    • , Laura van Weeren
    •  & Theodorus W J Gadella Jr

  • Research Highlights |

    A miniature head-mounted two-photon microscope small enough for a rat to carry allows researchers to visualize neuronal signaling while the animal freely interacts with its environment.

    • Daniel Evanko

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