Research Briefing |
Featured
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Research Briefing |
Spectral phasor analysis enables multiplexed microscopy with bioluminescent probes
Bioluminescent phasor is a new technology for multiplexed, excitation-free imaging at the microscale using luciferase–luciferin pairs. This platform can readily unmix the broad, overlapping emission spectra of bioluminescent reporters, making possible the dynamic tracking of cellular and molecular features over prolonged time periods.
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Article |
Multiplexed bioluminescence microscopy via phasor analysis
The combination of engineered probes and spectral phasor analysis overcomes long-standing challenges associated with bioluminescence detection at the microscale, enabling multiplexed, real-time imaging of cellular features without the need for excitation light.
- Zi Yao
- , Caroline K. Brennan
- & Jennifer A. Prescher
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Article |
Novel NanoLuc substrates enable bright two-population bioluminescence imaging in animals
NanoLuc substrates with improved solubility and bioavailability, hydrofurimazine and fluorofurimazine, strongly enhance bioluminescence signals in vivo and enable bright dual-color bioluminescent imaging with AkaLuc and AkaLumine.
- Yichi Su
- , Joel R. Walker
- & Michael Z. Lin
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This Month |
Elena Goun
Bioluminescence lights her way to measure glucose uptake in vivo, and why a chemist travels outside her comfort zone.
- Vivien Marx
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Article |
Bioluminescent-based imaging and quantification of glucose uptake in vivo
A bioluminescent glucose-uptake probe enables accurate, real-time, non-invasive longitudinal imaging of d-glucose absorption both in vitro and in vivo.
- Tamara Maric
- , Georgy Mikhaylov
- & Elena Goun
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Research Highlight |
Harnessing fungal bioluminescence
Researchers have developed the first fully genetically encodable eukaryotic bioluminescent system.
- Rita Strack
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Method to Watch |
Building up bioluminescence
Improved luciferases and luciferins are poised to shine.
- Rita Strack
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Brief Communication |
Red-shifted luciferase–luciferin pairs for enhanced bioluminescence imaging
Red-shifted luciferins and corresponding mutants of NanoLuc enable brighter bioluminescence imaging in vitro, in cells, and in deep tissues of living mice alone and in the context of the newly developed Antares2 BRET reporter.
- Hsien-Wei Yeh
- , Omran Karmach
- & Hui-wang Ai
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Research Highlights |
Luciferase gets deep and sensitive
A luciferin analog enables highly sensitive bioluminescent imaging from deep within biological tissue samples.
- Richard Pattison
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Tools in Brief |
A bright orange fluorescent protein for enhanced in vivo imaging
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Tools in Brief |
Lighting up root architecture
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Tools in Brief |
Bright cyan and orange probes for bioluminescence imaging
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Brief Communication |
A synthetic luciferin improves bioluminescence imaging in live mice
The synthetic firefly luciferase substrate CycLuc1 offers brighter bioluminescence and improved imaging in mouse models at lower doses than the standard D-luciferin.
- Melanie S Evans
- , Joanna P Chaurette
- & Stephen C Miller
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Tools in Brief |
Imaging when cells get together
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Tools in Brief |
BRET-FRET nanoparticles
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Correspondence |
Reply to "Neonatal desensitization does not universally prevent xenograft rejection"
- Claire M Kelly
- , Victoria H Roberton
- & Anne E Rosser
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Correspondence |
Neonatal desensitization does not universally prevent xenograft rejection
- Miroslaw Janowski
- , Anna Jablonska
- & Piotr Walczak
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Methods in Brief |
Exciting fluorescence with luminescence
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Article |
Firefly luciferase mutants as sensors of proteome stress
Destabilized mutants of firefly luciferase are characterized as sensors for protein homeostasis (proteostasis). Their use as tools for comparisons of proteostasis capacity is demonstrated in cells and in Caenorhabditis elegans.
- Rajat Gupta
- , Prasad Kasturi
- & Swasti Raychaudhuri
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Research Highlights |
Neurons light the way
Monitoring the activity of neurons in vivo in the freely behaving zebrafish larvae is now possible using bioluminescence, an approach with great potential for unveiling how neuronal networks control behavior.
- Erika Pastrana
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Correspondence |
A red-shifted Renilla luciferase for transient reporter-gene expression
- Andreas Markus Loening
- , Anca Dragulescu-Andrasi
- & Sanjiv Sam Gambhir
Photon-based communication between two neurons in Caenorhabditis elegans