Research Briefing |
Featured
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Article
| Open Access
Imaging brain tissue architecture across millimeter to nanometer scales
Mapping fixed brain samples with extracellular labeling and optical microscopy reveals synaptic connections.
- Julia M. Michalska
- , Julia Lyudchik
- & Johann G. Danzl
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Brief Communication
| Open Access
Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity
A dual illumination method reduces photobleaching for green and yellow fluorescent proteins.
- Lucie Ludvikova
- , Emma Simon
- & Agathe Espagne
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Research Briefing |
Augmenting electron microscopy with barcoded gene reporters
Genetically encoded concentric barcodes function as multiplexed electron microscopy (EM) gene reporters (EMcapsulins) for cell culture and in vivo models. These barcodes can be used to visualize gene expression information as automatically labeled overlays on volume EM data.
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Research Briefing |
Imaging the biological microcosmos with a tiny telescope
We present the Schmidt objective, a novel astronomy-inspired concept for designing multi-immersion microscope objectives with high numerical aperture, long working distance and large field of view. The Schmidt objective uses a spherical mirror and a correction plate to focus light. It is well suited for high-resolution imaging deep inside cleared biological samples.
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Research Briefing |
Imaging large cell populations with fast, automated super-resolution
Leveraging advances in hardware and probes, we have combined innovations in optics and algorithms to allow automated single-molecule-based super-resolution imaging at unprecedented throughput. This approach allows us to obtain nanoscale information from large cell populations, bridging the gap between imaging and indirect ensemble methods.
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Brief Communication |
Trans-segmental imaging in the spinal cord of behaving mice
A wearable macroscope enables cellular imaging across multiple spinal segments in behaving mice.
- Pavel Shekhtmeyster
- , Daniela Duarte
- & Axel Nimmerjahn
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Article
| Open Access
Three-dimensional structured illumination microscopy with enhanced axial resolution
Enhanced axial resolution for 3D SIM is achieved with deep learning or four-beam interference.
- Xuesong Li
- , Yicong Wu
- & Hari Shroff
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News & Views |
Optical microscopy gets down to angstroms
Super-resolution microscopy at ångström precision could pave the way to optical structural biology in cells.
- Giorgio Tortarolo
- & Suliana Manley
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Article
| Open Access
MINSTED nanoscopy enters the Ångström localization range
Sub-nanometer localization precision is achieved with all-optical microscopy.
- Michael Weber
- , Henrik von der Emde
- & Stefan W. Hell
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Research Briefing |
Combining a deep neural network with physical properties for super-resolution live imaging
We integrated the pre-characterized physical model of super-resolution (SR) microscopy into a deep neural network to guide the denoising of raw images for high-quality SR image reconstruction. This approach enabled us to investigate a wide variety of fragile and rapidly evolving bioprocesses at ultrahigh spatiotemporal resolution over extended imaging times.
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Article |
Rationalized deep learning super-resolution microscopy for sustained live imaging of rapid subcellular processes
Rationalized deep learning improves image reconstruction by incorporating prior knowledge of illumination patterns.
- Chang Qiao
- , Di Li
- & Dong Li
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Article
| Open Access
Real-time denoising enables high-sensitivity fluorescence time-lapse imaging beyond the shot-noise limit
DeepCAD-RT denoises fluorescence time-lapse images in real time.
- Xinyang Li
- , Yixin Li
- & Qionghai Dai
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Research Briefing |
Towards the non-invasive imaging of brain networks and functions at high resolution
A new study describes a microscope combining three-photon excitation and adaptive optics capable of high-resolution in vivo imaging of fine neuronal structures in the mouse cortex through the intact skull. The authors demonstrate the use of this platform to guide precise laser-mediated microsurgery and for accurate and sensitive functional calcium imaging.
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Article |
Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping
ALPHA-FSS enables high-resolution imaging deep in the mouse brain.
- Zhongya Qin
- , Zhentao She
- & Jianan Y. Qu
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Research Briefing |
Sustainable bioimaging using a fluorescent protein with unprecedented photostability
The jellyfish-derived green fluorescent protein StayGold is bright and hardly fades, contributing to improving spatiotemporal resolution and dramatically extending the observation period. To fully benefit from the rich photon budget, we tried some unusual illumination modalities for sustainable, quantitative live-cell or volumetric imaging.
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Article
| Open Access
A highly photostable and bright green fluorescent protein
StayGold is over one order of magnitude more photostable than current fluorescent proteins
- Masahiko Hirano
- , Ryoko Ando
- & Atsushi Miyawaki
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Article
| Open Access
Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging
Calcium and other analytes can be imaged at super-resolution and in vivo with photo-switchable sensors.
- Kanuj Mishra
- , Juan Pablo Fuenzalida-Werner
- & Andre C. Stiel
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Article |
Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy
The resolution of fluorescence microscopy is increased by incorporating prior information into deconvolution algorithms.
- Weisong Zhao
- , Shiqun Zhao
- & Liangyi Chen
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Article |
Highly-multiplexed volumetric mapping with Raman dye imaging and tissue clearing
Simultaneous, Raman-based imaging of more than 10 proteins in thick samples.
- Lixue Shi
- , Mian Wei
- & Wei Min
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Article |
Volumetric live cell imaging with three-dimensional parallelized RESOLFT microscopy
A super-resolution microscope images live cells in 3D with high speed, large fields of view and minimal photodamage.
- Andreas Bodén
- , Francesca Pennacchietti
- & Ilaria Testa
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Brief Communication |
Left-handed DNA-PAINT for improved super-resolution imaging in the nucleus
Left-handed DNA probes improve super-resolution DNA-PAINT imaging in the nucleus by reducing background.
- H. J. Geertsema
- , G. Aimola
- & H. Ewers
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Article |
Imaging volumetric dynamics at high speed in mouse and zebrafish brain with confocal light field microscopy
A new imaging method enables deep imaging of neural networks.
- Zhenkun Zhang
- , Lu Bai
- & Kai Wang
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Article |
Rapid image deconvolution and multiview fusion for optical microscopy
Microscopy datasets are processed orders-of-magnitude faster with improved algorithms and deep learning.
- Min Guo
- , Yue Li
- & Hari Shroff
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Letter |
Multiplex digital spatial profiling of proteins and RNA in fixed tissue
A turnkey system allows for spatial profiling of proteins and RNA in fixed tissues, providing a window on cellular heterogeneity.
- Christopher R. Merritt
- , Giang T. Ong
- & Joseph M. Beechem
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Article |
Protection of tissue physicochemical properties using polyfunctional crosslinkers
Controlled tissue fixation with polyepoxides protects protein fluorescence, antigenicity, nucleic acids and tissue architecture.
- Young-Gyun Park
- , Chang Ho Sohn
- & Kwanghun Chung
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Article |
Deep learning massively accelerates super-resolution localization microscopy
Accelerating PALM/STORM microscopy with deep learning allows super-resolution imaging of >1,000 cells in a few hours.
- Wei Ouyang
- , Andrey Aristov
- & Christophe Zimmer
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Article |
Fast, long-term, super-resolution imaging with Hessian structured illumination microscopy
An improved image reconstruction algorithm increases time resolution and maximal imaging time for super-resolution microscopy.
- Xiaoshuai Huang
- , Junchao Fan
- & Liangyi Chen
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Article |
Nanoscale imaging of clinical specimens using pathology-optimized expansion microscopy
Expansion microscopy, a technique for super-resolution imaging, is extended to clinical human tissue samples that are formalin fixed, paraffin embedded, stained and/or fresh frozen.
- Yongxin Zhao
- , Octavian Bucur
- & Edward S Boyden
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News & Views |
Let there be light—at the right place
A light-sheet microscope that automatically corrects optical aberrations enables quantitative imaging of deep tissue.
- Lars Hufnagel
- & Rainer Pepperkok
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Article |
Adaptive light-sheet microscopy for long-term, high-resolution imaging in living organisms
Adaptive light-sheet microscopy improves imaging of live organisms by correcting for optical aberrations in real time.
- Loïc A Royer
- , William C Lemon
- & Philipp J Keller
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News & Views |
Super-resolution microscopy writ large
Improved expansion microscopy provides a super-resolution method that is accessible to any laboratory with a fluorescence microscope.
- Peter Engerer
- , Caroline Fecher
- & Thomas Misgeld
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Letter |
Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies
Improved expansion microscopy method preserves signal from fluorescent proteins and antibodies using off-the-shelf reagents.
- Paul W Tillberg
- , Fei Chen
- & Edward S Boyden
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Article |
A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo
In vivo imaging is facilitated by a bright, cyan-excitable orange fluorescent protein that is the basis of an improved bioluminescent protein.
- Jun Chu
- , Younghee Oh
- & Michael Z Lin
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Commentary |
The smart and gentle microscope
New instruments are needed to realize the potential of quantitative and systematic imaging of living samples. But what would such a microscope look like?
- Nico Scherf
- & Jan Huisken
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News & Views |
Two views on light sheets
A dual-view light-sheet microscope combines isotropic spatial resolution with high speed and minimal phototoxicity.
- Carl G Ebeling
- & Erik M Jorgensen
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Article |
Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy
A dual-view light sheet microscope allows high-speed imaging with an isotropic spatial resolution
- Yicong Wu
- , Peter Wawrzusin
- & Hari Shroff
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Generating expression profiles of single living cells from Raman microscopy
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- Atomic force microscopy
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