Multiphoton microscopy articles within Nature Biotechnology

Featured

• Research Briefing | 31 March 2023

  Imaging the biological microcosmos with a tiny telescope

  We present the Schmidt objective, a novel astronomy-inspired concept for designing multi-immersion microscope objectives with high numerical aperture, long working distance and large field of view. The Schmidt objective uses a spherical mirror and a correction plate to focus light. It is well suited for high-resolution imaging deep inside cleared biological samples.

• Research Briefing | 13 June 2022

  Towards the non-invasive imaging of brain networks and functions at high resolution

  A new study describes a microscope combining three-photon excitation and adaptive optics capable of high-resolution in vivo imaging of fine neuronal structures in the mouse cortex through the intact skull. The authors demonstrate the use of this platform to guide precise laser-mediated microsurgery and for accurate and sensitive functional calcium imaging.

• Article | 13 June 2022

  Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping

  ALPHA-FSS enables high-resolution imaging deep in the mouse brain.

  • Zhongya Qin
  • , Zhentao She
  •  & Jianan Y. Qu

• Article | 04 October 2021

  Highly-multiplexed volumetric mapping with Raman dye imaging and tissue clearing

  Simultaneous, Raman-based imaging of more than 10 proteins in thick samples.

  • Lixue Shi
  • , Mian Wei
  •  & Wei Min

• Article | 30 May 2016

  A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo

  In vivo imaging is facilitated by a bright, cyan-excitable orange fluorescent protein that is the basis of an improved bioluminescent protein.

  • Jun Chu
  • , Younghee Oh
  •  & Michael Z Lin