Cryoelectron microscopy articles within Nature

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  • Article |

    Mass spectrometry and structural studies demonstrate the specific changes in protein composition that accompany the transition of ribosomes in zebrafish and Xenopus eggs from a dormant to an active state during early embryogenesis.

    • Friederike Leesch
    • , Laura Lorenzo-Orts
    •  & Andrea Pauli

  • Article
    | Open Access

    Cryo-electron microscopy structures of Staphylococcus aureus BlaR1 reveal dynamic signalling states regulating broad spectrum β-lactam antibiotic resistance through cleavage of the transcriptional repressor BlaI and induced expression of the β-lactamase blaZ and the β-lactam-resistant cell-wall transpeptidase mecA.

    • J. Andrew N. Alexander
    • , Liam J. Worrall
    •  & Natalie C. J. Strynadka

  • Article
    | Open Access

    Structural analysis of Cas12a2, a CRISPR-associated nuclease that nonspecifically cleaves ssRNA, ssDNA and dsDNA, reveals a complete activation pathway involved in the abortive infection system protecting cells against invasion.

    • Jack P. K. Bravo
    • , Thomson Hallmark
    •  & David W. Taylor

  • Article |

    Structures of the human METTL1–WDR4 complex are revealed, providing molecular insights into substrate recognition, modification and catalytic regulation by the N7-methylguanosine methyltransferase complex.

    • Jiazhi Li
    • , Longfei Wang
    •  & Richard I. Gregory

  • Article |

    RibosomeST—a ribosome with a specialized nascent polypeptide exit tunnel—cotranslationally regulates the folding of a subset of male germ-cell-specific proteins that are essential for the formation of sperm.

    • Huiling Li
    • , Yangao Huo
    •  & Jiahao Sha

  • Article |

    Mutations in the sodium/iodide symporter (NIS) cause congenital hypothyroidism, and our results yield insights into how NIS selects, couples and translocates anions, thereby establishing a framework for understanding NIS function.

    • Silvia Ravera
    • , Juan Pablo Nicola
    •  & Nancy Carrasco

  • Article |

    We report cryogenic electron microscopy structures of disc-shaped active NLRP3 oligomers in complex with NEK7 and ASC, and propose that the role of NEK7 is to transform NLRP3 into the active NLRP3 inflammasome disc.

    • Le Xiao
    • , Venkat Giri Magupalli
    •  & Hao Wu

  • Article
    | Open Access

    Structural studies of the CRISPR-associated transposon comprising Cas12k, TnsC, TnsB and TniQ from Scytonema hofmannii using cryo-electron microscopy reveal insights into the architecture and mechanism of RNA-guided DNA transposition.

    • Jung-Un Park
    • , Amy Wei-Lun Tsai
    •  & Elizabeth H. Kellogg

  • Article
    | Open Access

    Structural insights into the poly-ADP-ribosyltransferase tankyrase reveal its filamentous architecture and illustrate how assembly controls catalytic and non-catalytic functions.

    • Nisha Pillay
    • , Laura Mariotti
    •  & Sebastian Guettler

  • Article
    | Open Access

    The cryo-EM structure of the yeast SEA complex suggests that SEACAT functions as a scaffold for binding TORC1 regulators.

    • Lucas Tafur
    • , Kerstin Hinterndorfer
    •  & Robbie Loewith

  • Article |

    Electrophysiological, structural and biochemical studies on the bestrophin-2 anion channel reveal asymmetric permeability to glutamate and show that it forms a cooperative machinery in complex with glutamine synthetase for glutamate release.

    • Aaron P. Owji
    • , Kuai Yu
    •  & Tingting Yang

  • Article
    | Open Access

    Cryo-electron microscopy structures of skeletal F-actin show solvent-driven rearrangements governing actin filament assembly and aging with potential application in design of drugs and small molecules for imaging and therapy.

    • Wout Oosterheert
    • , Björn U. Klink
    •  & Stefan Raunser

  • Article |

    Two cyclic dinucleotide molecules bind within the cavity of human SLC19A1 as a compact dual-molecule unit, whereas folate and antifolate bind to SLC19A1 as a monomer and occupy a distinct pocket of the cavity.

    • Qixiang Zhang
    • , Xuyuan Zhang
    •  & Pu Gao

  • Article |

    Biochemical and structural analysis of intermediates during multipass membrane protein biogenesis showed how an intramembrane chaperone guides nascent membrane proteins to a semi-enclosed lipid-filled cavity where they are inserted and folded correctly.

    • Luka Smalinskaitė
    • , Min Kyung Kim
    •  & Ramanujan S. Hegde

  • Article |

    Cryo-electron microscopy structures of the mouse class M immunoglobulin B cell receptor complex provide insight into B cell receptor subunit assembly, domain architecture and signalling mechanism.

    • Ying Dong
    • , Xiong Pi
    •  & Hao Wu

  • Article
    | Open Access

    The cryogenic-electron microscopy structure of the D. thermocuniculi IsrB protein in complex with its cognate ωRNA and a target DNA shows that the RNA-dominant IsrB effector complex shares a common scaffold with the protein-dominant Cas9 effector complex.

    • Seiichi Hirano
    • , Kalli Kappel
    •  & Feng Zhang

  • Article |

    The cryo-electron microscopy structure of NuA4 from Saccharomyces cerevisiae bound to the nucleosome illustrates how NuA4 is assembled and provides mechanistic insights into nucleosome recognition and transcription co-activation by a histone acetyltransferase.

    • Keke Qu
    • , Kangjing Chen
    •  & Zhucheng Chen

  • Article |

    Structural studies of the Ebola virus polymerase complex provide insights into its function and demonstrate the structural basis of its inhibition by suramin.

    • Bin Yuan
    • , Qi Peng
    •  & Yi Shi

  • Article
    | Open Access

    Evolutionary conservation of plant receptor structure allowed for generation of new variants of wheat and barley nucleotide-binding leucine-rich repeat receptors (NLRs) that recognize AvrSr35 of the wheat stem rust pathogen, supporting proof of principle for structure-based engineering of NLRs for crop improvement.

    • Alexander Förderer
    • , Ertong Li
    •  & Jijie Chai

  • Article |

    A structural analysis focusing on plant immunity reveals how LRR-containing receptor-like proteins recognize pathogenic ligands and consequently become activated, with the data suggesting that these proteins target pathogens through two different mechanisms.

    • Yue Sun
    • , Yan Wang
    •  & Jijie Chai

  • Article |

    Cryo-electron microscopy studies of Escherichia coli complex I suggest a conserved mechanism of coupled proton transfers and electrostatic interactions that result in proton ejection from the complex exclusively at the distal NuoL subunit.

    • Vladyslav Kravchuk
    • , Olga Petrova
    •  & Leonid Sazanov

  • Article |

    Cryogenic electron microscopy analyses reveal a new, compact structure of telomeric chromatin, providing mechanistic insight into telomere maintenance and function.

    • Aghil Soman
    • , Sook Yi Wong
    •  & Lars Nordenskiöld

  • Article |

    Cryogenic electron microscopy structures of the Synechocystis phycobilisome—alone and bound with orange carotenoid protein—reveal detailed information regarding the biophysical basis of the control of cyanobacterial light harvesting.

    • María Agustina Domínguez-Martín
    • , Paul V. Sauer
    •  & Cheryl A. Kerfeld

  • Article |

    A bacterial antiviral defence system generates a cyclic tri-adenylate that binds to a TIR–SAVED effector, inducing formation of a superhelical structure with adjacent TIR domains organizing into an active site, allowing NAD+ degradation.

    • Gaëlle Hogrel
    • , Abbie Guild
    •  & Malcolm F. White

  • Article
    | Open Access

    Reconstitution of the SARS-CoV-2 RNA 5′ cap reveals the unconventional mechanism by which SARS-CoV-2 caps its RNA genome, providing a new target in the development of antiviral agents to treat COVID-19.

    • Gina J. Park
    • , Adam Osinski
    •  & Vincent S. Tagliabracci

  • Article |

    Thyroid-stimulating hormone and autoantibody M22 push the extracellular domain of the thyrotropin receptor into an upright active conformation, revealing a universal activation mechanism of glycoprotein hormone receptors and providing the molecular basis of Graves’ disease, hypothyroidism and Hashimoto’s disease.

    • Jia Duan
    • , Peiyu Xu
    •  & H. Eric Xu

  • Article
    | Open Access

    The nucleus-like compartment formed in bacteria during infection by jumbo phage 201phi2-1 is composed of the bacteriophage protein chimallin, which can self-assemble into closed compartments in vitro.

    • Thomas G. Laughlin
    • , Amar Deep
    •  & Elizabeth Villa

  • Article
    | Open Access

    Structures of the Arabidopsis thaliana auxin exporter PIN1 in the apo state, bound to the natural auxin or bound to an inhibitor provide insights into the polar auxin transport mechanisms mediated by PIN family transporters.

    • Zhisen Yang
    • , Jing Xia
    •  & Linfeng Sun

  • Article |

    Cryo-electron microscopy structures of the bacterial protein machinery that is involved in the production and function of nitrous oxide provide insight into the assembly pathway of this enzyme and the mechanisms of copper transport.

    • Christoph Müller
    • , Lin Zhang
    •  & Oliver Einsle

  • Article
    | Open Access

    Cryo-electron microscopy structures of IL-25–IL-17RB–IL-17RA and IL-17A–IL-17RC–IL-17RA complexes show a tip-to-tip architecture, which is a key organizing principle of the IL-17 receptor family.

    • Steven C. Wilson
    • , Nathanael A. Caveney
    •  & K. Christopher Garcia

  • Article
    | Open Access

    Through structural analysis of the activation of bacterial STING, the molecular basis of STING filament formation and TIR effector domain activation in antiphage signalling is defined.

    • Benjamin R. Morehouse
    • , Matthew C. J. Yip
    •  & Philip J. Kranzusch

  • Article |

    The cryo-electron microscopy structure of the filamentous hydrogen-dependent CO2 reductase (HDCR) enzyme from Thermoanaerobacter kivui, together with enzymatic analysis and in situ cryo-electron tomography, provides insight into the high catalytic activity of HDCR.

    • Helge M. Dietrich
    • , Ricardo D. Righetto
    •  & Jan M. Schuller

  • Article
    | Open Access

    The Csu pili of the multidrug-resistant nosocomial pathogen Acinetobacter baumannii assemble into an ultrathin zigzag architecture secured by a clinch mechanism that provides the pilus with high mechanical stability and superelasticity.

    • Natalia Pakharukova
    • , Henri Malmi
    •  & Anton V. Zavialov

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