Volume 18 Issue 3, March 2023

A protocol for purification, full-length sequencing and genomic origin mapping of extrachromosomal circle DNA using a three-step purification strategy combined with rolling-circle amplification and Oxford Nanopore sequencing.

IonStar is an integrated proteomics suite utilizing ultrahigh-resolution-MS1 acquisition to achieve high-quality quantification of large sample cohorts. IonStar encompasses strategies for sample preparation, liquid chromatography–mass spectrometry analysis and data processing.

This protocol describes the preparation of high-quality single-cell suspensions from the human placenta for downstream characterization, using droplet-based single-cell RNA sequencing platforms.

The Nodewalk assay converts chromosome conformation capture DNA samples to RNA and, subsequently, cDNA to enable ultrasensitive quantitative analyses of chromatin fiber interactions.

This protocol describes a solvothermal-based process to prepare gram-scale ferrite nanoparticles with well-defined shapes (nanocubes, nanostars, faceted and spherical) having heating properties appealing for clinical magnetic hyperthermia treatments.

This protocol describes an in vitro vesicle formation system to reconstitute, purify and characterize plant-derived coat protein complex II vesicles from Arabidopsis thaliana suspension-cultured cells.

This protocol uses PlantPegDesigner to design and optimize prime editing guide RNA and engineered plant prime editor vectors for efficient prime editing in monocot plants.

Microcrystals are increasingly being used for structural analysis. The challenge addressed by this protocol is to generate large numbers of high-quality crystals of uniform size for various experiments, including time-resolved studies of reactions.

This protocol combines shell-isolated nanoparticle-enhanced Raman spectroscopy and ab initio molecular dynamics simulations to unravel the directional molecular features of interfacial water, enabling a better understanding of electrocatalysis.

Here the authors provide a protocol for functional genomics using engineered symbionts (FUGUES) that utilizes an engineered bee gut bacterium to induce a sustained RNAi response in the host that can be used to study honey bee biology.

The comet assay is commonly used to assess DNA damage. This collection of consensus protocols includes adaptations for a wide range of species and sample types, assay formats and detection of different types of DNA lesions.

A micro-computed X-ray tomography-based approach for quantifying the number and volume of lung cancer nodules over time, enabling the tracking of individual nodule formation, tumor growth and response to therapy.