Volume 17 Issue 9, September 2022

This protocol allows users to quantify metal-bearing engineered nanomaterials in biological tissues and cells and to analyze their dynamic behavior at trace-level concentrations, using single-cell inductively coupled plasma mass spectrometry, single-particle inductively coupled plasma mass spectrometry and synchrotron X-ray absorption fine structure.

We present a protocol for building a scanning light-field microscope with digital adaptive optics as an add-on to a standard wide-field microscope to achieve long-term, high-speed intravital fluorescence imaging.

Poly(A) inclusive full-length RNA isoform sequencing is a method for measuring poly(A) tail length and composition at single-mammalian-oocyte (subnanogram)-level sensitivity.

The development of nucleoside analogs for use as antiviral agents and antisense oligonucleotides requires efficient synthetic methods. This three-step approach starts with proline-catalyzed fluorination of simple heteroaryl-substituted aldehydes.

This protocol describes the working principle and implementation of axially swept light-sheet microscopy, which can image subcellular features at organ scales in 3D. It discusses how to optimize its design and provides a detailed guide for its construction and alignment.

Treatments developed in small-animal models of stroke are often not translatable to the clinic. In this protocol, a blood clot in a microcatheter is introduced into the middle cerebral artery of rhesus monkeys; controlled thrombolysis is possible.

The functions of circular RNAs are challenging to investigate because of high sequence similarity to their cognate linear mRNAs. This protocol outlines a strategy for specific targeting of circular RNAs in genome-wide pooled CRISPR–Cas13 screens.

The authors describe a protocol for growing a tumor of mouse or human origin beneath the skin of a mouse, which is widely known as the subcutaneous mouse tumor model.