Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
The iBEAT V2.0 is a robust, multisite-applicable, infant-tailored computational pipeline that uses deep learning to process and analyze infant brain magnetic resonance images. This protocol describes the usage and technique details of the iBEAT V2.0.
The authors present a protocol for preparation—by dehydration and mounting—of whole human organs for imaging via X-ray hierarchical phase-contrast tomography, resulting in high-resolution images with a ratio of voxel size to organ diameter of 1:150,000.
EPAM-ia is a method based on flow cytometry for the simultaneous isolation and analysis of endothelial cells, pericytes, astrocytes and microglia from the neurovascular unit, a structure that critically regulates blood–brain barrier function.
The authors present a simple and cost-efficient approach for the covalent coupling of enzymes on solid supports or for the intermolecular cross-linking of enzymes.
This protocol describes cfSNV, a user-friendly software package that comprehensively considers the unique properties of cell-free DNA for the sensitive detection of somatic mutations from blood samples.
In many environmental or clinical settings, it is useful to detect and quantify analytes instantaneously in situ. This protocol describes how to develop metal–organic frameworks for selective, sensitive analysis by luminescence.
In this Perspective, authors from the UK Consortium on Metabolic Phenotyping propose a ‘fit-for-purpose’, four-tiered framework to evaluate the reliability of targeted metabolomics analyses, addressing the need for community-accepted, harmonized guidelines for tiers other than full validation.
A robust, mild and fast approach for the posttranslational, site-directed fluorination of protein sidechains, detectable via 19F-based magnetic resonance methods.
The authors present a protocol for the generation of human blastoids—structures morphologically and transcriptionally resembling complete blastocysts—from pluripotent stem cells and for their use in modeling implantation into hormonally stimulated endometrial cells.
This protocol for concomitant high-throughput mitochondrial DNA genotyping and accessible chromatin profiling of single cells allows paired analysis of clonal relationships and cell states.
The authors present a protocol for the modular 3D bioengineering of multilineage skeletal muscles from human induced pluripotent stem cells, along with assays to characterize morphological and functional features of the artificial muscle constructs.
The tandem mass spectrometers used in clinical chemistry are expensive. This protocol describes how to generate similar results using a single mass spectrometry detector by optimizing in-source fragmentation and data analysis via correlated ion monitoring.
The comet assay is commonly used to assess DNA damage. This collection of consensus protocols includes adaptations for a wide range of species and sample types, assay formats and detection of different types of DNA lesions.
This protocol describes a solvothermal-based process to prepare gram-scale ferrite nanoparticles with well-defined shapes (nanocubes, nanostars, faceted and spherical) having heating properties appealing for clinical magnetic hyperthermia treatments.
Single-cell screening and sorting is useful for addressing many biological questions. The high-throughput droplet analyzer and sorter described in this protocol has been designed so that it can be constructed without specialist engineering training.
A full-body nanobody-based immunolabeling and clearing method that renders mice transparent in 3 weeks, enhancing the signal of fluorescent proteins and allowing their reliable detection and quantification, at cellular resolution, within an entire body.
We present a protocol for the synthesis and usage of QM-FN-SO3, a near-infrared aggregation-induced-emission-active fluorescent probe capable of crossing the blood–brain barrier and ultrasensitively lighting up amyloid-β plaques in living mice.
This Protocol Extension presents an updated tetrahedral DNA nanostructure for the delivery of various bioactive molecules that enables an active targeting strategy to be used for stimuli-sensitive conformation changes and on-site cargo release.