This protocol enables users to establish long-term multigenerational ctenophore cultures in the laboratory and details CRISPR–Cas9 genome editing in Mnemiopsis leidyi.

This protocol guides the user through normative modeling analysis using the Predictive Clinical Neuroscience toolkit (PCNtoolkit), enabling individual differences to be mapped at the level of a single subject or observation in relation to a reference model.

This protocol details the synthesis and use of Peroxymycin-1, an activity-based histochemical probe for hydrogen peroxide detection in fixed cell and mouse tissue samples.

Mouse or human organoids are generated from two cervical regions: columnar epithelium-lined endocervix and stratified squamous epithelium-lined ectocervix. Genetic manipulation for modeling cancers and organoid infections is also described.

We present a protocol for building a scanning light-field microscope with digital adaptive optics as an add-on to a standard wide-field microscope to achieve long-term, high-speed intravital fluorescence imaging.

This protocol allows users to quantify metal-bearing engineered nanomaterials in biological tissues and cells and to analyze their dynamic behavior at trace-level concentrations, using single-cell inductively coupled plasma mass spectrometry, single-particle inductively coupled plasma mass spectrometry and synchrotron X-ray absorption fine structure.

Treatments developed in small-animal models of stroke are often not translatable to the clinic. In this protocol, a blood clot in a microcatheter is introduced into the middle cerebral artery of rhesus monkeys; controlled thrombolysis is possible.

It is crucial to know the localization of a protein to fully understand its function. SubCellBarCode is a wet lab–, mass spectrometry- and bioinformatics-based pipeline that generates proteome-wide protein subcellular localization for human cancer cell lines.

LC–HRMS is used for metabolomics studies in the biomedical and environmental sciences. MetaboAnalyst (metaboanalyst.ca) can be used to address challenges in data processing, statistical analysis, functional interpretation and multi-omics integration.

This protocol enables users to establish long-term multigenerational ctenophore cultures in the laboratory and details CRISPR–Cas9 genome editing in Mnemiopsis leidyi.

Fluorescently labeled nuclei of the enteric nervous system are isolated from adult mouse or zebrafish gut and characterized by nuclear RNA sequencing and visualization and quantification of transcripts using a custom analytical pipeline.

Sulc and colleagues describe a design tool, oxView, for visualization and analysis of simulations of DNA, RNA and nucleic acid–protein nanostructures.

This protocol describes the preparation of two lipid-based membrane–core nanoformulations for FOLFOX by using the nanoprecipitation technique. These can be used in the treatment of colorectal cancer and hepatocellular carcinoma.