News & Views

New computational method uses convolutional neural networks for cis-regulatory sequence analysis to analyze and cluster scATAC-seq data.

Dipole–dipole crosstalk between fluorophores separated by a distance of less than 10 nm induces changes in their photophysics, which adds a challenge to localization microscopy in the sub-10-nm regime.

A diagnostic fragment ion in tandem mass spectrometry enables confident protein lactylation assignment and the discovery of broad lysine modification beyond histones.

Evidence for at least one protein product from 80% of all mouse genes is reported in a comprehensive proteomic analysis of 41 adult mouse tissues. Comparison of tissue profiles between mouse and human suggests that the fundamental biology of this important model organism is even more different from our own than we thought.

New data collection methods enable ab initio protein structure determination by micro-electron diffraction for the first time.

De novo structure generation from tandem mass spectrometry data will advance annotation of unknown metabolomic signals.

A repeat-aware pipeline corrects structural assembly errors and polishes assembly sequences in large repeats without overcorrection.

A novel bright near-infrared fluorescent protein inserted into a nanobody enables visualization of native proteins inside living cells and specific manipulation of cell function, including Boolean protein-based operators.

DiMeLo-seq leverages immunotethered DNA methyltransferases with long-read sequencing to map the locations of chromatin proteins in their natural context.

A novel approach to probabilistically align adjacent multiple tissue slices from spatially resolved transcriptomics data provides unprecedented depth for the investigation of tissue architecture and paves the way for new developments in 3D spatial analytics.

A flexible open-top light-sheet microscope has been developed that can perform deep three-dimensional imaging on all clearing protocols with low and high optical resolution.

Two new toolkits that leverage deep-learning approaches can track the positions of multiple animals and estimate poses in different experimental paradigms.

Engineered viral entry combined with single-cell sequencing technology makes it possible to identify specific ligand–receptor interactions in a high-throughput manner.

Global profiling of changes in the reactivity of cysteine residues in response to phosphorylation during mitosis identifies cysteine residues as potential regulatory and drug binding sites on proteins.

A droplet microfluidic system enables deterministic capture and subsequent sequencing and analysis of single-cell transcriptomes from organoids and other small, individual tissue samples.

Cryofixation-based ultrastructure-expansion microscopy (cryo-ExM) bypasses artifacts caused by chemical fixation and establishes more-native preservation of biological samples.

Introducing iTracer, a system for long-term measurement of lineage dynamics at high resolution.

As last we can edit the immune system’s sleeping giants, as CRISPR tools advance into the world of naive CD4⁺ T cells.

How a decades-old vaccine for yellow fever may help scientists study the brain connectome.

Software solutions pGlyco3 and StrucGP both aim to better assign the glycan part of a glycopeptide beyond simple glycosyl composition, but they differ in their strategies, their requirement for a glycan library and their applicability to O-glycopeptides.