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Advancing multiplexed imaging for enhanced tissue complexity analysis

Nature Methods volume 20, pages 1280–1281 (2023)Cite this article

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Multiplexed spatial immunophenotyping has advanced our understanding of tissues in the context of homeostasis and disease. Two studies now provide additional tools to overcome challenges with multiplexed imaging: one procedure amplifies the detection of low-abundance antigens by integrating SABER and IMC technologies, and the other is an X-ray-based method that enables the non-destructive multiplexed detection of antigens in tissues at scalable resolution and speed.

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Fig. 1: SABERx-IMC and MEZ-XRF are solutions for challenges in IMC.

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Acknowledgements

N.F.C.C.d.M. is funded by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (grant agreement no. 852832).

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Authors and Affiliations

  1. Pathology, Leiden University Medical Center, Leiden, The Netherlands

    Marieke E. Ijsselsteijn & Noel F. C. C. de Miranda

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  1. Marieke E. Ijsselsteijn
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  2. Noel F. C. C. de Miranda
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Correspondence to Noel F. C. C. de Miranda.

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Ijsselsteijn, M.E., de Miranda, N.F.C.C. Advancing multiplexed imaging for enhanced tissue complexity analysis. Nat Methods 20, 1280–1281 (2023). https://doi.org/10.1038/s41592-023-01935-7

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