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Genetics

Discovering CRISPR–Cas off-target breaks

Nature Methods volume 20, pages 641–642 (2023)Cite this article

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DISCOVER-seq+ enables the efficient detection of CRISPR–Cas off-target breaks in primary cells and tissues.

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Fig. 1: DISCOVER-seq+ increases the detection of CRISPR–Cas off-target sites in a variety of model systems.

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Acknowledgements

We thank M. Bienko (Human Technopole and Karolinska Institutet) for providing insightful comments and suggestions. This work was supported by funding from the Swedish Research Council (grant. no. 2022-00721) to N.C. and by intramural funding from Human Technopole to C.J. and N.C.

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Authors and Affiliations

  1. Human Technopole, Milan, Italy

    Carlos Jiménez & Nicola Crosetto

  2. Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden

    Nicola Crosetto

  3. Science for Life Laboratory, Solna, Sweden

    Nicola Crosetto

Authors
  1. Carlos Jiménez
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  2. Nicola Crosetto
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Correspondence to Nicola Crosetto.

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Jiménez, C., Crosetto, N. Discovering CRISPR–Cas off-target breaks. Nat Methods 20, 641–642 (2023). https://doi.org/10.1038/s41592-023-01847-6

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