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Unlocking cryo-EM’s multishot potential with square or rectangular beams

Nature Methods volume 21, pages 555–557 (2024)Cite this article

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New condenser aperture designs form square or rectangular beams that match the camera dimensions, which efficiently expands the data acquisition area in cryogenic electron microscopy.

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Fig. 1: Illustration of multishot data acquisition using round and square or rectangular beam.

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Acknowledgements

I thank Z. Yu and N. Spellmon from cryo-EM shared resource, Janelia Research Campus, HHMI, for the helpful comments and discussions.

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  1. Cryo-EM shared resource, Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA

    Xiaowei Zhao

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  1. Xiaowei Zhao
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Correspondence to Xiaowei Zhao.

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The author declares no competing interests.

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Zhao, X. Unlocking cryo-EM’s multishot potential with square or rectangular beams. Nat Methods 21, 555–557 (2024). https://doi.org/10.1038/s41592-024-02224-7

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