Abstract
Oxygen deficiency is a major problem in the Baltic Sea. To study the impact of hypoxia on the functional diversity of benthic fauna and the possibility of macrozoobenthos recovery, data were analyzed in a gradient of oxygen conditions in the Gdańsk Basin. The research conducted on the basis of biological traits analysis enabled us to analyze the number, type and spatial distribution of biological traits—a proxy for functions performed by macrozoobenthos. A significant depletion of macrofauna was already observed under conditions of reduced oxygen above the bottom, both in terms of functional diversity and biomass. Although taxa observed in hypoxia (DO < 2 mL L−1) perform a number of functions, the remaining species do not form complex structures in the sediments or cause deep bioturbation and bioirrigation. Moreover, their extremely low biomass plays an irrelevant role in benthic–pelagic coupling. Thus, benthic fauna under hypoxia is not an element that ensures the functioning of the ecosystem. We assess that traits important for species dispersal and the presence of taxa resistant to short-term hypoxia in the oxic zone above the halocline provide a “backup” for ecosystem functioning under altered diverse oxygen conditions below the halocline after cessation of hypoxia in the southern Baltic Sea.
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Introduction
Oxygen deficiency is a natural or human-induced phenomenon observed in water bodies around the world1,2. Some of the largest marine anaerobic areas occur in the Baltic Sea3,4. In the deep-water basins of this sea, oxygen deficiencies occur on a long-term basis and are related to the occurrence of strong water stratification and the lack of mixing of well-oxygenated surface waters with bottom waters5. They are also the result of perennially high terrigenous nutrient loads and consequently a high influx of organic matter into the sediments6. Seasonal or episodic oxygen deficiencies have been recorded in coastal waters7,8,9,10,11. Research shows that ongoing climate change contributes to the deterioration of oxygen conditions in seas and oceans12. The results of mathematical modeling indicate that future temperature increases will, inter alia, contribute to an increase in the area affected by hypoxia and anoxia in the central Baltic Sea, and that the duration of their impact will be prolonged13,14. However, hypoxia can be reduced through further reductions in nutrient loads14.
Oxygen depletion affect both the functioning of individual organisms and entire communities, but can also cause changes in ecosystem functioning15,16,17. One of the mechanisms involved in the response to adverse environmental conditions is the escape of mobile fauna from exposed areas18. Organisms that are unable to escape respond by changing their behavior, e.g., increasing the rate of ventilation19, while infauna emerge to the sediment surface20,21. As a result of insufficient levels of dissolved oxygen (DO) in the environment, physiological and biochemical changes are observed in invertebrates, first by maintaining oxygen delivery and transportation (e.g., increasing the respiration rate), then by decreasing the metabolic rate and switching to anaerobic metabolism15,22,23. Recurrent hypoxia causes, inter alia, changes in enzyme activity, which results in reduced effectiveness of the immune system24,25.
Seasonal episodic hypoxia is known to reduce the number of species, their abundance and biomass26,27,28. Small body size, rapid growth rates, as well as reduced bioturbation and annual life cycles are traits of species inhabiting such zones29,30. The presence of such species, also characterized by high tolerance to oxygen depletion and the content of H2S, determines the functioning of the bottom of this zone, i.e., the resistance of the ecosystem to hypoxia. There are also species that, while exhibiting low resistance to hypoxia, have characteristics that allow them to quickly colonize the zone when oxygen conditions improve (e.g., mobility). The presence of such species in close proximity to an area exposed to oxygen deficiency enables the ecosystem to recover, i.e., increases its resilience to hypoxia.
Studies of ecological functioning can be based on ecological relations of individual organisms, such as trophic relationships31,32, or the ability to modify the environment through animal activity33,34. Benthic communities form an interaction matrix of biological traits that drive ecosystem functions (understood as ecosystem processes, i.e., production, decomposition and nutrient cycling) and condition responses to environmental drivers35. Therefore, functioning can also be assessed by studying the number, type and spatial distribution of functions performed by organisms in an ecosystem36. Biological traits analysis (BTA) is one of the methods used to analyze ecosystem functioning35,37,38,39. The BTA approach uses a number of selected traits of organisms related to their role in the functioning of benthic communities in the ecosystem.
Studies of functional diversity have been conducted in the Baltic Sea both by analyzing individual biological traits and by analyzing the occurrence of functions throughout the ecosystem38,39,40,41,42,43,44. The impact of oxygen deficiency on the functioning of macrozoobenthos has been analyzed in laboratory studies conducted on individual species20,21,45 and, rarely, on communities in experiments21,46,47 or on given communities in the environment48,49,50,51. By collecting data under different oxygen conditions in the Gdańsk Basin, we can fill in the gaps in our knowledge about the transformations of communities under such conditions. The objective of our research was to determine the impact of different oxygen conditions on the structure and, consequently, on the functioning of benthic communities in the southern Baltic Sea. We investigated which functions of the benthic macrofauna in the southern Baltic Sea are constrained by adverse oxygen conditions and which remain represented? And finally, we assessed whether the oxic zone above the halocline provides “backup” for the functioning of the ecosystem below the halocline—a depleted zone under reduced or hypoxic conditions.
Results
Bottom–water characteristics
Three groups of sites were defined based on the oxygen condition: normoxia with good oxygen conditions (DO ≥ 4); reduced of oxygen conditions (DO 2–4); and hypoxia (DO < 2). Dissolved oxygen in bottom water ranged from 4.11 to 8.41 mL L−1 in the group of normoxic sites, from 2.55 to 3.91 mL L−1 at sites with reduced oxygen conditions, and from 0.63 to 1.59 mL L−1 at hypoxic sites (Table 1). The salinity of bottom water at the analyzed sites (Fig. 1) was typical of the Gdańsk Basin and increased with depth from 6.7 (20 m) to a maximum of 13.8 (107 m; Table 1).
Taxonomic richness and biomass of macrofauna
A total of 34 species or higher taxa were identified at all the surveyed sites. The highest number of taxa (18) was recorded at a depth of 35 m (site GN). In general, the number of taxa per site decreased with increasing depth and deterioration of oxygen conditions (Fig. 2). The fewest or complete absence of macrozoobenthos taxa was recorded in the two groups of sites below the halocline, statistically significantly less than in normoxia group (Kruskal–Wallis test p < 0.0001, H = 23.698, df = 2; post-hoc test p < 0.001). Between five and 18 taxa (on average 11) were present at individual sites in the shallowest part with DO ≥ 4.0 mL L−1, and all taxa recorded during this research were present at the sites in this group. From zero to six species were present at the sites where oxygen concentration was lower than that under normoxia and under hypoxia, with a total of nine and four taxa recorded, respectively. No macrofauna was recorded at three sites, and only the polychaete Bylgides sarsi was present at five other sites.
Under good oxygen conditions, the biomass at individual sites ranged from 67.4 g m−2 (site PB39) to 546.3 g m−2 (site VE48) and averaged 188.2 g m−2. The bivalve Macoma balthica accounted for the largest proportion of biomass (Fig. 3), averaging 163.3 g m−2 (85%). Eight other taxa contributed > 1% to the biomass: the priapulid worm Halicryptus spinulosus, polychaetes Marenzelleria spp., Hediste diversicolor, bivalves Cerastoderma glaucum, Mya arenaria, Mytilus trossulus, gastropods from Hydrobiinae, and the crustacean Pontoporeia femorata (Fig. 3). Below 80 m the biomass was significantly lower than that under good aerobic conditions, averaging 34.8 g m−2 under deteriorated oxygen conditions and was drastically lower under hypoxia (0.3 g m−2). There was significant difference in community structure (characterized by the biomass of taxa) between all groups (ANOSIM global test R = 0.704, p = 0.001) and in the pairwise test between the normoxia group and the reduced oxygen conditions group (ANOSIM pairwise test R = 0.833, p = 0.001), as well as between the normoxic group and the hypoxic group (pairwise test R = 1, p = 0.001).
The highest average similarity (SIMPER) between the sites in each group (56%) was observed under good oxygen conditions, where the taxa responsible for the similarity between the sites were M. balthica (82%) and, to a much lesser extent, Marenzelleria spp. (4%), M. arenaria (3%) and H. diversicolor (2%). The internal similarity at sites with a DO range of 2.0–4.0 mL L−1 was 24%, with 98% of this similarity attributed to the presence of B. sarsi. Under hypoxia, the similarity was 24%, which was solely due to the presence of B. sarsi. The average difference between the group of normoxic sites and other groups was higher than 88%.
Biological traits and functional diversity
The number of represented modalities decreased with the deterioration of oxygen conditions. At the sites with normoxia, all 66 analyzed modalities of the 16 biological traits were represented by macrozoobenthos (Table 2). Between 50 and 64 categories were represented at individual sites under good oxygen conditions. Fewer, 53 modalities (80%), were recorded under reduced oxygen conditions. Between 19 and 35 modalities were represented at sites with DO < 2.0 mL L−1, where fauna was present. In the group of hypoxic sites, none of the modalities were represented (present) in more than 60% of the samples (grabs) from the sites in this group. At the same time, very rare and rare modalities (represented by one or up to two taxa, respectively) were recorded in all groups. Under normoxia, five modalities were identified as very rare and four as rare. At the sites from the group under reduced oxygen conditions, nine modalities were rare, and 13 modalities were represented by one taxon. At the same time, of all 42 modalities represented under hypoxia, 18 modalities were represented by only one taxon, and 17 were represented by two taxa.
The macrofauna biomass and selected biological traits, as well as dissolved oxygen concentration at each site, are presented in the graphs (Fig. 4). The percentage of individual biological traits in the total macrofauna biomass varied at the sites characterized by good oxygen conditions. Traits reflecting the impact of organisms on sediment structure and nutrient concentrations in pore water (Bioturbation method, Environmental position) indicate a diverse and strong impact under normoxic conditions, but little or no effect under other conditions (Fig. 4b,d). In the case of another effect trait, Longevity, very long-lived organisms dominated at normoxic sites, while short-lived ones dominated in the other groups. As for traits relevant to species dispersal (Fig. 4e–g), such as Reproduction frequency, which is important for colonization of new areas, annual episodic and annual protracted predominated under good aerobic conditions. The latter dominated under lower oxygen conditions (Fig. 4e). Under good oxygen conditions, more than half of the biomass consisted of non-dispersal and local organisms, dispersing within a distance of 10–100 m (Fig. 4g). Both groups of deteriorated oxygen conditions included local or long-distance migrating organisms.
The FD index presented in Fig. 5 for the sampling sites is a measure of dissimilarity between taxa based on traits and relative biomass of taxa in the community.
The values and variability of FD index were higher in good oxygen group compared to the other groups. The median FD index at the sites under normoxia was 0.12 ± 0.10, which was an order of magnitude higher than in the group of sites with reduced oxygen conditions (0.03 ± 0.07) and the group of hypoxic sites (0.06 ± 0.07; Fig. 5).
Discussion
In the soft sediments of Gdańsk Basin, oxygen is the main factor affecting the structure and biomass of benthic communities. Its deterioration follows a gradient of increasing depth, as a consequence of decreasing sediment grain size and increasing organic matter content. Additionally, toxic hydrogen sulfide is released during decomposition processes. Under good oxygen conditions, diverse and abundant benthic fauna communities occur on all types of sediments—from coarse-grained sands with low organic matter content to silts with much higher organic matter content71.
Zoobenthic communities, representing the largest number of biological traits, were observed in the zone above the halocline with good oxygen conditions in bottom waters. All 66 analyzed modalities were observed above the halocline. Törnroos et al.39 reported a comparable richness of modalities (common in theirs and our study) of biological traits for communities from the southern Baltic Sea. Taxonomic richness in the Baltic Sea decreases due to the salinity gradient52, which leads to an overall reduction in number of trait categories represented, but functional richness remains relatively high even at the lowest level of taxon richness39. In the deterioration of oxygen conditions in the Gdańsk Basin, we observed a 34% decline in modalities. In all groups, we observed very rare and rare modalities (i.e., represented by one or a maximum of two taxa, respectively). While in the zone characterized by good oxygen conditions, they accounted for only about 14% of all modalities represented, at oxygen-depleted sites they accounted for 58%, and under hypoxia as much as 83% of the modalities present were represented by up to two taxa. According to the redundancy hypothesis53, there is a minimum level of diversity to ensure the functioning of an ecosystem. In addition to those providing the minimum, the remaining taxa constitute a kind of protection in case of possible disturbances53, such as periodic oxygen deficiency at the bottom. In the case of the southern Baltic deep water region, there is no such protection, as only single taxa with extremely low biomass occur there.
In addition to the richness of biological traits, the FD index is also important when describing functional diversity. In our case, the FD index is a proxy for the dissimilarity between traits, which includes proportion of a biomass in the community. The highest FD index (maximum of 0.31) was observed at the normoxic sites, with a median value of 0.12, which is five times higher compared to the depleted sites. Due to the low biomass of macrofauna below the halocline, the lack of diversification of traits between the taxa forming a community, and the strong dominance of a single taxon at the depleted and hypoxic sites, the median FD index was very low and dropped to 0 at the sites with one taxon. All the obtained values were lower than the results obtained in the western Baltic Sea (range 0.12–0.56), where the recorded number of taxa was significantly higher for most of the study period48. Studies of benthic communities at the soft seabed of the Baltic Sea showed that FD followed a decline in taxonomic richness39. The relatively low FD values observed at the normoxic sites are due to high average similarity of the benthic communities of soft-bottom habitats due to the strong dominance (> 85%) of M. balthica in the total biomass, which is typical in Gdańsk Basin26,28,58. Research in the Aegean Sea has also shown that the FD index (maximum of 0.30) is higher in areas with diverse habitats54.
Hypoxia alters ecosystem functioning by reducing the number of species, density or biomass, or even the total loss of zoobenthos1,16,29. With the loss of taxa, the traits they represent are also lost to the ecosystem, and individual traits reflect the functions performed by organisms in a given ecosystem. For example, the maximum size of adult specimens relates to i.a. trophic relationships, which in turn affects the circulation of matter in an ecosystem55. The lifespan of organisms is considered a proxy for energy fixation, turnover and production rate56. In the Gulf of Gdańsk, organisms ranging from very small to the largest were recorded under normoxic conditions, while only species growing to intermediate size as adult maxima, were present under deteriorated oxygen conditions and hypoxia. In addition, the frequency of organisms in all lifespan modalities is lower in oxygen-deficient regions. The obtained results corroborate the work by Pacheco et al.30,57, who recorded large and medium-sized organisms as well as those with a lifespan of up to 12 year in shallow-water (15 m) habitats in Mejillones Bay (northern Chile). While at a depth of 50 m, in an oxygen-deficient region, only small (but not the smallest) taxa, with a lifespan of several years were observed. In the brackish waters of the Baltic Sea, oxygen-deficient regions are dominated by small species that burrow only in the surface layer of sediments, including zones where large, perennial, deep-burrowing taxa were previously observed58,59. Among the taxa recorded in the Gulf of Gdańsk, long-lived taxa include bivalves. Other taxa live from several months (i.a. small polychaetes and crustaceans) to several years (e.g., priapulid worms, large polychaetes). The size of detritivores determines the size of the food particles they choose60,61 and, at the same time, along with their environmental position, their vulnerability to predator attacks. Of the organisms recorded in this study, the largest (> 50 mm) were adults of selected polychaete species, e.g., Marenzelleria spp., the bivalve mollusk M. arenaria and the crustacean S. entomon. They provide a food base for numerous species of fish62,63 and diving birds64,65. The smallest specimens were those of taxa recorded only under normoxia, such as Jaera spp., Hydrobiinae and Fabricia stellaris. These taxa constitute a food base for benthic invertebrates66 and fish67, including juveniles. Consequently, oxygen deficiency in the seabed zone causes changes and disruptions in the circulation of matter, while the reduction in the size and complexity of benthic food webs alters and disrupts energy pathways1,20.
With regard to ecosystem functioning, a separate group consists of traits directly affecting the biotope, categorized by de Juan et al.35 as effect traits. Among traits related to the role played by organisms directly in the sediment matrix (i.a. environmental position and type of bioturbation), all functional categories were recorded only under good oxic conditions. As aerobic conditions deteriorate, species that are important in organic matter processing—penetrating deep into the sediment, forming complex biogenic structures in the sediment, feeding on subsurface detritus and carrying particles from deeper layers of the sediment to its surface (matter conveyors)—disappear. While the presence of infauna in the upper 2 cm of the sediment was still detectable under deteriorated oxygen conditions, only epifaunal and bentho-pelagic taxa, feeding on detritus on the sediment surface, as well as crawling and swimming organisms were found under hypoxic conditions. These results correspond with previous observations by other authors indicating that as oxygen conditions deteriorate, taxa that penetrate deep layers of the sediment, forming biogenic structures there and feeding on subsurface detritus, as well as sediment conveyors, are lost first29,30,48,68. Even short-term, but often recurring hypoxia leads to a significant reduction in the bioturbation potential of benthic communities through, among other things, removal of large deep burrowing individuals47,69. Under oxic conditions, fauna has a significant impact on solute fluxes50 and can increase the removal of phosphate and silicate from sediments into the bottom water70. Most of the species recorded in our study are associated with the surface layer of the sediment, but some taxa, such as M. balthica, M. arenaria and polychaetes Marenzelleria spp., are able to burrow deep into the sediment71, given that deep burrowers are adults and the largest individuals. Most individuals, including juveniles, occur on the surface layer of the sediment71,72. Organisms responsible for transporting and mixing sediment particles contribute, among other things, to the transfer of fresh organic matter from the surface into deeper layers of the sediment73, and by oxygenating interstitial water, among other things, increase the rate of mineralization of organic matter in the sediments74,75. Moreover, through the disruption of the structure of macrofauna communities and species burial depth, oxygen deficiency affects biogeochemical processes47,69,70,76,77.
Persistent hypoxia leads to a decline in the biodiversity of macrofauna, while the possibility of returning to a state enabling proper functioning (recovery) is facilitated by the presence of undisturbed communities in the immediate vicinity, i.e., the recovery potential of the ecosystem after hypoxia78. The taxonomic richness and density of the macrofauna occurring in the zone above the halocline constitutes a potential reserve for the recovery of benthic communities when oxygen conditions improve in deeper regions. According to the category of response traits35 (Table 2), reproduction, locomotion and dispersal traits are essentials for recovery. Reproductive traits, such as methods and frequency of reproduction, are important for, inter alia, the possibility of restoring benthic communities in post-disturbance areas48,79. Our study shows that under normoxia, organisms reproduce both sexually and asexually. At sites belonging to other groups, almost exclusively sexually reproducing species were observed, in contrast to the results reported from northern Chile30,80, where only asexually reproducing polychaetes were observed in oxygen-deficient zones. Sexual reproduction provides genetic variation and thus increases a system resilience and recovery potential after disturbance81, but there is no external supply of benthic propagule into the deep habitat of the Chilean coast30. The presence of organisms with a long reproductive season in the ecosystem is also important for the process of succession after a disturbance, as they are more likely to colonize the region if conditions improve82. Organisms representing all reproduction frequencies were recorded above the halocline, including numerous taxa that reproduce for several months of the year, such as M. balthica. While macrozoobenthos was present in the area of the Gdańsk Deep and its slope in the past58, not only has no macrozoobenthos been observed in recent years, but hydrogen sulfide may be present in the water above the sediment up to nine months of the year83. We recorded two taxa in this area—polychaetes B. sarsi and juveniles (length < 5 mm) of M. balthica. While B. sarsi quickly colonizes areas as soon as oxygen conditions improve there48,84, M. balthica, on the other hand, takes several months to grow to a few millimeters, so its individuals are indicators of good oxygen conditions for at least several months before harvesting. The latter species reproduces in the southern Baltic Sea in spring and autumn85. The presence of both taxa is due to a series of major water inflows into the Baltic Sea that occurred in 2014–201586. Macrozoobenthos was no longer recorded in samples collected from the Gdańsk Deep in 2016.
Regeneration is also determined by the available pool of mobile colonizers in undisturbed regions82. The mode of locomotion and dispersal in the environment affects the production or dispersal potential of organisms as well as their colonization potential37,48,56. Under fully oxygenated conditions, species with diverse (including long-distance) dispersal habits are found. Experimental field studies87 conducted in shallow water areas showed that the first colonizers of the experimental area were adult immigrants settling at the top sediment layer. While adult crustaceans, gastropods or polychaetes, due to their vertical migration, can successfully colonize a previously disturbed area47, adult M. balthica or M. arenaria are not be expected there. According to Beukema et al.88, colonization of post-hypoxic areas by macrofauna is a complex process—most taxa colonize through settling pelagic larvae or juveniles, as in the case of taxa with simple development, but colonization by adults also occurs. On the other hand, research conducted in the environment after natural and human-induced improvement of oxygen conditions in, among others, the fjords of western Sweden indicated colonization by settling larvae, mobile bentho-pelagic organisms and epifauna78,89. Research on succession after the cessation of oxygen deficiency unanimously indicates that an increase in species richness and density occurs rapidly (from several summer months to a year), but biomass comparable to that in undisturbed areas and the occurrence of very large individuals was recorded only after several years78,90.
The rate of recolonization is also determined by spatial scale82, the impact of other disturbances such as excess organic matter or recurrent oxygen deficiency69. However, the return of large bivalve or shellfish individuals, including long-lived sedentary species, depends on long-term improvement in oxygen conditions. Although it is potentially possible for organisms to form benthic communities once adverse oxygen conditions cease91, this involves a change in the structure of the entire community in favor of taxa that have adaptations to conditions of reduced oxygen concentration and hydrogen sulfide92. In the zone above the halocline, which allows the recovery of macrofauna in deeper regions, taxa with high tolerance to oxygen deficiency were observed, including H. spinulosus, Marenzelleria spp., S. entomon and M. balthica, provided that these are not long-term events22,45,93,94.
Although ecosystem resistance depends, among other factors, on the composition of benthic communities and their resilience95,96, ecosystem recovery depends primarily on the improvement of oxygen conditions. In studies of the recovery potential of macrozoobenthos, it is also necessary to consider other biological response traits that enable the survival under these adverse conditions (including having large energy reserves that can be used in anaerobic metabolism) and the availability of planktic larvae.
As oxygen conditions deteriorated, the biomass, diversity and functionality of the macrozoobenthos declined markedly. Although a few taxa observed under hypoxia, their biomass was extremely low. Both very small and large organisms penetrating deep into the sediments disappear in depleted conditions. In the area under hypoxic conditions, we observe only small, mobile organisms with high turnover of energy and less stable storage of carbon and nitrogen compared to large, long-lived fauna. On the positive site, they can spread quickly when oxygen conditions improve. However, these species do not form complex structures in the sediments and do not cause bioturbation and bioirrigation in deeper sediment layers. Thus, animals are not able to enhance ecosystem functions as production, decomposition or nutrient cycling. Traits important for species dispersal and the presence of taxa temporarily resistant to hypoxia indicate that benthic communities above the halocline may be a potential source of organisms for recolonization of deeper areas in the southern Baltic Sea.
Methods
Study area
The research was carried out in the Gdańsk Basin, in the southern Baltic Sea. The basin consists of the shallower Gulf of Gdańsk in the south and the adjacent Gdańsk Deep, with a maximum depth of 114 m. A seasonal thermocline occurs in this area at a depth of about 30–40 m, and a halocline is usually observed at a depth of about 60–80 m. The bottom sediments of the Gdańsk Basin have a complex structure—sands or mixed sandy sediments in the shallow waters, silts in the central part of the Gulf Gdańsk, and clayey-silty sediments in the Gdańsk Deep97. The Gdańsk Basin is strongly impacted by the Vistula River, which is a great source of nutrients and organic matter to the system98,99,100. In general, oxygen conditions in the depth zones are typical for this basin: those above the halocline are good throughout the year26. Episodic oxygen deficiencies are observed in the zone below the halocline26,28,99,101. In the Gdańsk Deep, on the other hand, starting from its slope (at a depth of approximately 80 m), oxygen deficiencies occur regularly, even for several months a year3,102,103. Anoxic conditions with hydrogen sulfide in the bottom water were observed in the Gdańsk Deep area almost every year in last two decades3.
Sampling and environmental data
Thirty-four sampling sites were located in a gradient of oxygen conditions in the bottom zone in the depth range of 20–107 m (Fig. 1). Samples were collected during several cruises between 2009 and 2016 (Supplementary Table S1). Data for two sites collected during HELCOM monitoring in 2015 were obtained from ICES Database DOME104. Water parameters were analyzed approximately 0.5 m above the seabed. Bottom water temperature and salinity were measured instantaneously using a Seabird CTD system or a multi-parameter 340i WTW Gmb meter. Dissolved oxygen (DO) concentration was measured using Winkler titration or a Seabird SBE43 oxygen sensor (Supplementary Table S1).
Biological data analysis
Samples of macrozoobenthos for species and functional diversity analysis were collected using a standard method with a 0.1 m2 Van Veen grab sampler (1 mm sieve), with 1–5 replicates collected at each site. The residual material remaining on the sieve was preserved with 4% formaldehyde solution. Organisms were identified to the lowest taxonomic unit and counted, and their formalin wet weight was determined to the nearest 0.1 mg. Oligochaeta, polychaetes Marenzelleria, mud snails Hydrobiinae, crustaceans Jaera, juveniles of Idotea and larvae of Chironomidae were not identified to the species level.
Hypoxic conditions are understood differently by different authors and the effects of oxygen deficiency on organisms vary for different species or even life stages of specific species92,105. We have adopted the definition of hypoxia from Diaz and Rosenberg1 as oxygen concentrations below 2 mL L−1, while anoxic conditions refer to the lack of oxygen in the water. Lower oxygen levels become harmful not only when there is hypoxia in the environment, but also when reduced oxygen conditions cause behavioral and physiological responses, such as reduced growth, loss of reproductive capacity, mortality, etc.15,20,91,106. To determine the macrofauna characteristics in relation to oxygen conditions, the sites were divided into three groups based on the concentration of dissolved oxygen in bottom water at the surveyed sites during sampling: normoxia DO ≥ 4.0 mL L−1; deterioration of oxygen conditions DO 2.0–3.9 mL L−1; and hypoxia DO < 2.0 mL L−1 (Supplementary Table S1).
BTA—biological traits analysis
Biological traits analysis (BTA) was used to determine the functional diversity of macrofauna. Each taxon was analyzed and classified based on 16 selected biological traits (Table 2), within which 66 modalities (categories) were distinguished. The classification was based on information available in peer-reviewed scientific literature and databases (i.a.48,107,108,109; Supplementary Table S2). Biological traits modalities were assigned to the lowest possible taxonomic level. The fuzzy coding approach110 was performed based on the affinity of each taxon to the trait. It was scored from 0 to 1, where 0 indicates no affinity of a taxon to a trait category and 1 indicates absolute affinity38.
The number of taxa representing particular modality in every oxic group was counted. Trait modalities were considered rare if they were represented by two taxa in a given group, while modalities that were represented by one taxon were classified as very rare. The frequency of each functional modality in the groups of sites was analyzed based on the presence/absence of each modality in individual samples (grabs).
FD—Functional Diversity index
Rao's quadratic entropy (generalization of Simpson’s diversity index) was used as a measure of functional diversity (FD) of community. The FD index was determined in the free macro “FunctDiv.xls”111, based on the matrices: taxa by traits modalities and biomass of taxa.
For each trait, FD is a measure of distances between pairs of taxa in terms of trait modalities they represent in the entire community, expressed by relative biomass at given site. The FD index is the average value of FD calculated for individual traits, with the range from 0 to 1. If there is a single taxon or all taxa in the community have exactly the same traits, the FD has a minimum value of 0. If every pair of species in the community is completely different in terms of traits, the index reaches the highest value and is equal Simpson’s diversity index (expressed as 1 minus Simpson’s index of dominance).
Statistical analysis
Analysis of Similarities (ANOSIM) was used to determine differences in the taxonomic composition of benthic invertebrate communities and macrofauna biomass at the sampling sites, followed by SIMPER similarity analysis (Similarity Percentages) to assess which taxa were responsible for an observed difference between groups. Prior to similarity analysis, the biomass of individual taxa was transformed (√)112. The nonparametric multivariate Kruskal–Wallis test was used along with post-hoc pairwise comparisons to compare the number of taxa between the sites from the oxygen groups. The analyses were performed using Dell Statistica 13.1 and Primer 7 (PRIMER-E Ltd).
Spatial representation of the results on maps was performed in ArcGIS Pro 2.6.0 ESRI Inc.
Data availability
The datasets generated and/or analyzed during the study are available from the corresponding author on reasonable request.
References
Diaz, R. J. & Rosenberg, R. Spreading dead zones and consequences for marine ecosystems. Science 321, 926–929 (2008).
Rabalais, N. N. et al. Dynamics and distribution of natural and human-caused hypoxia. Biogeosciences 7, 585–619 (2010).
Hansson, M. & Andersson, L. Oxygen Survey in the Baltic Sea 2021 - Extent of Anoxia and Hypoxia, 1960–2021. Report Oceanography 72, (2021).
Fennel, K. & Testa, J. M. Biogeochemical controls on coastal hypoxia. Annu. Rev. Mar. Sci. 11, 105–130 (2019).
Rolff, C., Walve, J., Larsson, U. & Elmgren, R. How oxygen deficiency in the Baltic Sea proper has spread and worsened: The role of ammonium and hydrogen sulphide. Ambio 51, 2308–2324 (2022).
Meier, H. E. M. et al. Disentangling the impact of nutrient load and climate changes on Baltic Sea hypoxia and eutrophication since 1850. Clim. Dyn. 53, 1145–1166 (2019).
Conley, D. J. et al. Hypoxia is increasing in the coastal zone of the Baltic Sea. Environ. Sci. Technol. 45, 6777–6783 (2011).
Andersson, L. S. Hydrography and oxygen in the deep basins. HELCOM Baltic Sea Environment Fact Sheets. https://helcom.fi/baltic-sea-trends/environment-fact-sheets/hydrography/ (2014). Accessed 15 March 2016.
Carstensen, J., Andersen, J. H., Gustafsson, B. G. & Conley, D. J. Deoxygenation of the Baltic Sea during the last century. Proc. Natl. Acad. Sci. USA 111, 5628–5633 (2014).
Naumann, M. et al. Hydrographic-hydrochemical assessment of the Baltic Sea 2017. Marine Sci. Rep. 107 (2018).
Brzana, R., Janas, U. & Tykarska, M. B. Effects of a 70-year old artificial offshore structure on oxygen concentration and macrobenthos in the Gulf of Gdańsk (Baltic Sea). Estuar. Coast. Shelf Sci. 235, 106563. https://doi.org/10.1016/j.ecss.2019.106563 (2020).
Reusch, T. B. H. et al. The Baltic Sea as a time machine for the future coastal ocean. Sci. Adv. 4, eaar8195. https://doi.org/10.1126/sciadv.aar8195 (2018).
Neumann, T. et al. Extremes of temperature, oxygen and blooms in the Baltic Sea in a changing climate. Ambio 41, 574–585 (2012).
Meier, H. E. M., Dieterich, C. & Gröger, M. Natural variability is a large source of uncertainty in future projections of hypoxia in the Baltic Sea. Commun. Earth Environ. 2, 50 (2021).
Wu, R. S. S. Hypoxia: from molecular responses to ecosystem responses. Mar. Pollut. Bull. 45, 35–45 (2002).
Karlson, K., Rosenberg, R. & Bonsdorff, E. Temporal and spatial large-scale effects of eutrophication and oxygen deficiency on benthic fauna in Scandinavian and Baltic waters—a review. Oceanography Mar. Biol. Annu. Rev. 40, 427–489 (2002).
Conley, D. J. et al. Hypoxia-related processes in the Baltic Sea. Environ. Sci. Technol. 43, 3412–3420 (2009).
Rabalais, N. N., Harper, D. E. & Turner, R. E. Responses of nekton and demersal and benthic fauna to decreasing oxygen concentrations. In Coastal Hypoxia: Consequences for Living Resources and Ecosystems, Coastal and Estuarine Studies 58, 115–128 (2001).
Hagerman, L. & Szaniawska, A. Respiration, ventilation and circulation under hypoxia in the glacial relict Saduria (Mesidotea) entomon. Mar. Ecol. Prog. Ser. 47, 55–63 (1988).
Diaz, R. J. & Rosenberg, R. Marine benthic hypoxia: a review of its ecological effects and the behavioural responses of benthic macrofauna. Oceanography Mar. Biol. Annu. Rev. 33, 245–303 (1995).
Villnäs, A., Norkko, J., Lukkari, K., Hewitt, J. & Norkko, A. Consequences of increasing hypoxic disturbance on benthic communities and ecosystem functioning. PLoS ONE 7, e44920. https://doi.org/10.1371/journal.pone.0044920 (2012).
Oeschger, R. Long-term anaerobiosis in sublittoral marine invertebrates from the Western Baltic Sea: Halicryptus spinulosus (Priapulida), Astarte borealis and Arctica islandica (Bivalvia). Mar. Ecol. Prog. Ser. 59, 133–143 (1990).
Normant, M. & Szaniawska, A. Behaviour, survival and glycogen utilisation in the baltic isopod Saduria entomon exposed to long-term oxygen depletion. Mar. Freshw. Behav. Physiol. 33, 201–211 (2000).
Philipp, E. E. R. et al. Gene expression and physiological changes of different populations of the long-lived bivalve Arctica islandica under low oxygen conditions. PLoS ONE 7, e44621. https://doi.org/10.1371/journal.pone.0044621 (2012).
Villnäs, A., Norkko, A. & Lehtonen, K. K. Multi-level responses of Macoma balthica to recurring hypoxic disturbance. J. Exp. Mar. Biol. Ecol. 510, 64–72 (2019).
Janas, U., Wocial, J. & Szaniawska, A. Seasonal and annual changes in the macrozoobenthic populations of the Gulf of Gdańsk with respect to hypoxia and hydrogen sulphide. Oceanologia 46, 85–102 (2004).
Rakocinski, C. F. & Menke, D. P. Seasonal hypoxia regulates macrobenthic function and structure in the Mississippi Bight. Mar. Pollut. Bull. 105, 299–309 (2016).
Warzocha, J., Gromisz, S., Wodzinowski, T. & Szymanek, L. The structure of macrozoobenthic communities as an environmental status indicator in the Gulf of Gdańsk (the Outer Puck Bay). Oceanologia 60, 553–559 (2018).
Levin, L. A. et al. Effects of natural and human-induced hypoxia on coastal benthos. Biogeosciences 6, 2063–2098 (2009).
Pacheco, A. S. et al. Functional diversity of marine macrobenthic communities from sublittoral soft-sediment habitats off northern Chile. Helgol. Mar. Res. 65, 413–424 (2011).
Pearson, T. H. Functional group ecology in soft sediment marine benthos: the role of bioturbation. In Oceanography and marine biology (eds Gibson, R. N. et al.) 233–267 (Taylor & Francis, 2001).
Kiljunen, M. et al. Benthic-pelagic coupling and trophic relationships in northern Baltic Sea food webs. Limnol. Oceanogr. 65, 1706–1722 (2020).
Thoms, F. et al. Impact of Macrofaunal communities on the coastal filter function in the Bay of Gdansk. Baltic Sea. Front. Mar. Sci. 5, 1–19 (2018).
Janas, U., Burska, D., Kendzierska, H., Pryputniewicz-Flis, D. & Łukawska-Matuszewska, K. Importance of benthic macrofauna and coastal biotopes for ecosystem functioning—Oxygen and nutrient fluxes in the coastal zone. Estuar. Coast. Shelf Sci. 225, 106238. https://doi.org/10.1016/j.ecss.2019.05.020 (2019).
de Juan, S. et al. Biological traits approaches in benthic marine ecology: Dead ends and new paths. Ecol. Evol. 12, e9001. https://doi.org/10.1002/ece3.9001 (2022).
Díaz, S. & Cabido, M. Vive la différence: plant functional diversity matters to ecosystem processes. Trends Ecol. Evol. 16, 646–655 (2001).
Bremner, J., Paramor, O. A. L. & Frid, C. L. J. Developing a methodology for incorporating ecological structure and functioning into designation of special areas of Conservation (SAC) in the 0–12 Nautical Mile Zone. A report to English Nature from University of Liverpool, Liverpool, 153 s. (2006).
Törnroos, A. & Bonsdorff, E. Developing the multitrait concept for functional diversity. Ecol. Appl. 22, 2221–2236 (2012).
Törnroos, A. et al. Marine benthic ecological functioning over decreasing taxonomic richness. J. Sea Res. 98, 49–56 (2015).
Bonsdorff, E. & Pearson, T. H. Variation in the sublittoral macrozoobenthos of the Baltic Sea along environmental gradients: A functional-group approach. Aust. J. Ecol. 24, 312–326 (1999).
Boström, C., O’Brien, K., Roos, C. & Ekebom, J. Environmental variables explaining structural and functional diversity of seagrass macrofauna in an archipelago landscape. J. Exp. Mar. Biol. Ecol. 335, 52–73 (2006).
Darr, A., Gogina, M. & Zettler, M. L. Functional changes in benthic communities along a salinity gradient—a western Baltic case study. J. Sea Res. 85, 315–324 (2014).
Törnroos, A. et al. Four decades of functional community change reveals gradual trends and low interlinkage across trophic groups in a large marine ecosystem. Global Change Biol. 25, 1235–1246 (2019).
Villnäs, A. et al. Changes in macrofaunal biological traits across estuarine gradients: implications for the coastal nutrient filter. Mar. Ecol. Prog. Ser. 622, 31–48 (2019).
Modig, H. & Ólafsson, E. Responses of Baltic benthic invertebrates to hypoxic events. J. Exp. Mar. Biol. Ecol. 229, 133–148 (1998).
Nilsson, H. & Rosenberg, R. Hypoxic response of two marine benthic communities. Mar. Ecol. Prog. Ser. 115, 209–217 (1994).
Norkko, A., Villnäs, A., Norkko, J., Valanko, S. & Pilditch, C. Size matters: Implications of the loss of large individuals for ecosystem function. Sci. Rep. 3, 2646. https://doi.org/10.1038/srep02646 (2013).
Gogina, M., Darr, A. & Zettler, M. L. Approach to assess consequences of hypoxia disturbance events for benthic ecosystem functioning. J. Mar. Syst. 129, 203–213 (2014).
Norkko, J. et al. Seafloor ecosystem function relationships: In situ patterns of change across gradients of increasing hypoxic stress. Ecosystems 18, 1424–1439 (2015).
Gammal, J., Norkko, J., Pilditch, C. A. & Norkko, A. Coastal hypoxia and the importance of benthic macrofauna communities for ecosystem functioning. Estuaries Coasts 40, 457–468 (2017).
Norkko, J. et al. Ecosystem functioning along gradients of increasing hypoxia and changing soft-sediment community types. J. Sea Res. 153, 1–12 (2019).
Zettler, M. L., Schiedek, D. & Bobertz, B. Benthic biodiversity indices versus salinity gradient in the southern Baltic Sea. Mar. Pollut. Bull. 55, 258–270 (2007).
Bolam, S. G., Fernandes, T. F. & Huxam, M. Diversity, biomass, and ecosystem processes in the marine benthos. Ecol. Monogr. 72, 599–615 (2002).
Dimitriadis, C., Evagelopoulos, A. & Koutsoubas, D. Functional diversity and redundancy of soft bottom communities in brackish waters areas: Local vs regional effects. J. Exp. Mar. Biol. Ecol. 426–427, 53–59 (2012).
Jennings, S., Warr, K. J. & Mackinson, S. Use of size-based production and stable isotope analyses to predict trophic transfer efficiencies and predator-prey body mass ratios in food webs. Mar. Ecol. Prog. Ser. 240, 11–20 (2002).
Weigel, B., Blenckner, T. & Bonsdorff, E. Maintained functional diversity in benthic communities in spite of diverging functional identities. Oikos 125, 1421–1433 (2016).
Pacheco, A. S., Uribe, R. A., Thiel, M., Oliva, M. E. & Riascos, J. M. Dispersal of post-larval macrobenthos in subtidal sedimentary habitats: Roles of vertical diel migration, water column, bedload transport and biological traits’ expression. J. Sea Res. 77, 79–92 (2013).
Osowiecki, A. & Warzocha, J. Macrobenthos of the Gdansk, Gotland and Bornholm Basins in 1978–1993. Oceanological Studies 1, 137–149 (1996).
Olenin, S. Benthic zonation of the Eastern Gotland Basin, Baltic Sea. Netherlands J. Aquatic Ecol. 30, 265–282 (1997).
Whitlatch, R. B. Patterns of resource utilization and coexistence in marine intertidal deposit-feeding communities. J. Mar Res. 38, 743–765 (1980).
Lopez, G. R. & Levinton, J. S. Ecology of deposit-feeding animals in marine sediments. Q. Rev. Biol. 62, 235–260 (1987).
Pihl, L. Changes in the Diet of Demersal Fish due to Eutrophication-Induced Hypoxia in the Kattegat, Sweden. Can. J. Fish. Aquatic Sci. 51, 321–336 (1994).
Dziaduch, D. Diet composition of herring (Clupea harengus L.) and cod (Gadus morhua L.) in the southern Baltic Sea in 2007 and 2008. Oceanol. Hydrobiol. Stud. 40, 96–109 (2011).
Stempniewicz, L. Feeding ecology of the Long-tailed Duck Clangula hyemalis wintering in the Gulf of Gdańsk (southern Baltic Sea). Ornis Svec. 5, 133–142 (1995).
Kube, J. Spatial and temporal variations in the population structure of the soft- shell clam Mya arenaria in the Pomeranian Bay (southern Baltic sea). J. Sea Res. 35, 335–344 (1996).
Janas, U. & Barańska, A. What is the diet of Palaemon elegans a non-indigenous species in the Gulf of Gdańsk (southern Baltic Sea)?. Oceanologia 50, 221–237 (2008).
Borg, J. P. G., Westerbom, M. & Lehtonen, H. Sex-specific distribution and diet of Platichthys flesus at the end of spawning in the northern Baltic Sea. J. Fish Biol. 84, 937–951 (2014).
Arntz, W. E. Zonation and dynamics of macrobenthos biomass in an area stressed by oxygen deficiency. In Stress Effects on Natural Ecosystems (eds Barrett, G. W. & Rosenberg, R.) 215–225 (Wiley, 1981).
Villnäs, A. et al. The role of recurrent disturbances for ecosystem multifunctionality. Ecology 94, 2275–2287 (2013).
Kendzierska, H., Łukawska-Matuszewska, K., Burska, D. & Janas, U. Benthic fluxes of oxygen and nutrients under the influence of macrobenthic fauna on the periphery of the intermittently hypoxic zone in the Baltic Sea. J. Exp. Mar. Biol. Ecol. 530–531 (2020).
Miernik, N. A., Janas, U. & Kendzierska, H. Role of macrofaunal communities in the Vistula River plume, the Baltic Sea—Bioturbation and bioirrigation potential. Biol. Basel 12, 147. https://doi.org/10.3390/biology12020147 (2023).
Zettler, M. L., Bick, A. & Bochert, R. Distribution and population dynamics of Marenzelleria viridis (Polychaeta, Spionidae) in a coastal water of the southern Baltic. Arch. Fish. Mar. Res. 42, 209–224 (1995).
Josefson, A. B., Norkko, J. & Norkko, A. Burial and decomposition of plant pigments in surface sediments of the Baltic Sea: Role of oxygen and benthic fauna. Mar. Ecol. Prog. Ser. 455, 33–49 (2012).
Karlson, K., Hulth, S., Ringdahl, K. & Rosenberg, R. Experimental recolonisation of Baltic Sea reduced sediments: Survival of benthic macrofauna and effects on nutrient cycling. Mar. Ecol. Prog. Ser. 294, 35–49 (2005).
Sun, M. Y. & Dai, J. Relative influences of bioturbation and physical mixing on degradation of bloom-derived particulate organic matter: Clue from microcosm experiments. Mar. Chem. 96, 201–218 (2005).
Lohrer, A. M., Thrush, S. F. & Gibbs, M. M. Bioturbators enhance ecosystem function through complex biogeochemical interactions. Nature 431, 1092–1095 (2004).
Solan, M. et al. Extinction and ecosystem function in the marine benthos. Science 306, 1177–1180 (2004).
Rosenberg, R., Agrenius, S., Hellman, B., Nilsson, H. C. & Norling, K. Recovery of marine benthic habitats and fauna in a Swedish fjord following improved oxygen conditions. Mar. Ecol. Prog. Ser. 234, 43–53 (2002).
Van Der Linden, P. et al. A biological trait approach to assess the functional composition of subtidal benthic communities in an estuarine ecosystem. Ecol. Indic. 20, 121–133 (2012).
Bon, M. et al. Functional changes in benthic macrofaunal communities along a natural gradient of hypoxia in an upwelling system. Mar. Pollut. Bull. 164, 112056. https://doi.org/10.1016/j.marpolbul.2021.112056 (2021).
Vásquez, C., Quiñones, R. A., Brante, A. & Hernández-Miranda, E. Genetic diversity and resilience in benthic marine populations. Revista Chilena de Historia Natural 96, 4. https://doi.org/10.1186/s40693-023-00117-1 (2023).
Zajac, R. N., Whitlatch, R. B. & Thrush, S. F. Recolonization and succession in soft-sediment infaunal communities: the spatial scale of controlling factors. Hydrobiologia 375, 227–240 (1998).
Kruk-Dowgiałło, L. & Szaniawska, A. Gulf of Gdańsk and Puck Bay. In Ecology of Baltic Coastal Waters. Ecological Studies Vol. 197 (ed. Schiewer, U.) 139–165 (Springer, Berlin, 2008).
Stigebrandt, A., Rosenberg, R., Magnusson, M. & Linders, T. Oxygenated deep bottoms beneath a thick hypoxic layer lack potential of benthic colonization. Ambio 47, 106–109 (2018).
Pierścieniak, K., Grzymała, J. & Wołowicz, M. Differences in reproduction and condition of Macoma balthica and Mytilus trossulus in the Gulf of Gdańsk (Southern Baltic Sea) under anthropogenic influences. Oceanol. Hydrobiol. Stud. 39, 17–32 (2010).
Rak, D. The inflow in the Baltic Proper as recorded in January–February 2015. Oceanologia 58, 241–247 (2016).
Gamenick, I., Jahn, A., Vopel, K. & Giere, O. Hypoxia and sulphide as structuring factors in a macrozoobenthic community on the Baltic Sea shore: Colonisation studies and tolerance experiments. Mar. Ecol. Prog. Ser. 144, 73–85 (1996).
Beukema, J. J., Flach, E. C., Dekker, R. & Starink, M. A long-term study of the recovery of the macrozoobenthos on large defaunated plots on a tidal flat in the Wadden Sea. J. Sea Res. 42, 235–254 (1999).
Stigebrandt, A. et al. An Experiment with forced oxygenation of the deepwater of the anoxic By Fjord, western Sweden. Ambio 44, 42–54 (2015).
Beukema, J. J. Expected changes in the Wadden Sean benthos in a warmer world: Lessons from periods with mild winters. Netherlands J. Sea Res. 30, 73–79 (1992).
Timmermann, K. et al. Modelling macrofaunal biomass in relation to hypoxia and nutrient loading. J. Mar. Syst. 105–108, 60–69 (2012).
Vaquer-Sunyer, R. & Duarte, C. M. Thresholds of hypoxia for marine biodiversity. Proc. Natl. Acad. Sci. USA 105, 15452–15457 (2008).
Schiedek, D., Vogan, C., Hardege, J. & Bentley, M. Marenzelleria cf. wireni (Polychaeta: Spionidae) from the Tay estuary. Metabolic response to severe hypoxia and hydrogen sulphide. Aquat. Ecol. 31, 211–222 (1997).
Long, W. C., Brylawski, B. J. & Seitz, R. D. Behavioral effects of low dissolved oxygen on the bivalve Macoma balthica. J. Exp. Mar. Biol. Ecol. 359, 34–39 (2008).
Boesch, D. F. & Rosenberg, R. Response to stress in marine benthic communities. In Stress effects on natural ecosystems (eds Barrett, G. W. & Rosenberg, R.) 179–200 (Wiley, 1981).
Llansó, R. J. Effects of hypoxia on estuarine benthos: the lower Rappahannock River (Chesapeake Bay), a case study. Estuar. Coast. Shelf. Sci. 35, 491–515 (1992).
Uścinowicz, S. Basen Gdański. Przegląd Geologiczny 45, 589–594 (1997).
Pastuszak, M. & Igras, J. Temporal and spatial differences in emission of nitrogen and phosphorus from Polish territory to the Baltic Sea. Gdynia-Puławy. NMFRI (2012).
Łukawska-Matuszewska, K., Kiełczewska, J. & Bolałek, J. Factors controlling spatial distributions and relationships of carbon, nitrogen, phosphorus and sulphur in sediments of the stratified and eutrophic Gulf of Gdansk. Cont. Shelf. Res. 85, 168–180 (2014).
Szczepanek, M., Silberberger, M. J., Koziorowska-Makuch, K. & Kedra, M. Utilization of riverine organic matter by macrobenthic communities in a temperate prodelta. Front. Mar. Sci. 9, 974539. https://doi.org/10.3389/fmars.2022.974539 (2022).
Brodecka, A., Majewski, P., Bolałek, J. & Klusek, Z. Geochemical and acoustic evidence for the occurrence of methane in sediments of the Polish sector of the southern Baltic Sea. Oceanologia 55, 951–978 (2013).
Graca, B. et al. Pore water phosphate and ammonia below the permanent halocline in the south-eastern Baltic Sea and their benthic fluxes under anoxic conditions. J. Mar. Syst. 63, 141–154 (2006).
Schmidt, B. et al. Long-term variability of near-bottom oxygen, temperature, and salinity in the Southern Baltic. J.M.S. 213, 103462. https://doi.org/10.1016/j.jmarsys.2020.103462 (2021).
ICES Marine Environmental Database (DOME), File accession 20161980.IMWP2015ZB_2023Jun12.PL. ICES, Copenhagen, Denmark. https://dome.ices.dk (2023) Accessed 02 February 2024.
Rousi, H., Korpinen, S. & Bonsdorff, E. Brackish-Water Benthic Fauna under fluctuating environmental conditions: The role of eutrophication, hypoxia, and global change. Front. Mar. Sci. 6, 464. https://doi.org/10.3389/fmars.2019.00464 (2019).
Galic, N., Hawkins, T. & Forbes, V. E. Adverse impacts of hypoxia on aquatic invertebrates: A meta-analysis. Sci. Total Environ. 652, 736–743 (2019).
Bremner, J. Assessing ecological functioning in marine benthic communities. PhD Thesis. (Newcastle University, 2005).
Nordström, M. C. Coastal food web structure and function: trophic interactions in shallow soft bottom communities. Ph.D. Thesis. (Åbo Akademi University, 2009).
Törnroos, A. Interpreting marine benthic ecosystem functioning in coastal waters: validating the biological trait concept. Ph.D. Thesis. (Åbo Akademi University, 2014).
Chevenet, F., Doléadec, S. & Chessel, D. A fuzzy coding approach for the analysis of long-term ecological data. Freshw. Biol. 31, 295–309 (1994).
Lepš, J., de Bello, F., Lavorel, S. & Berman, S. Quantifying and interpreting functional diversity of natural communities: practical considerations matter. Preslia 78, 481–501 (2006).
Clarke, K. R. & Gorley, R. N. PRIMER v6: User Manual/Tutorial. PRIMER-E, Plymouth (2006).
Acknowledgements
We would like to thank captains, scientific and operational crews of the research vessels and Polish Navy Ship ORP Heweliusz for their help during the collection of samples. We also thank E. Bonsdorff and A. Törnroos-Remes for detailed and fruitful discussions about functioning of Baltic macrofauna and K. Bradtke for statistical consultation. We would like to thank Institute of Meteorology and Water Management, Department of Oceanography and Baltic Monitoring, Gdynia, Poland for providing monitoring data from two sites from 2015 via the ICES. The authors would like to thank two anonymous reviewers for their detailed comments and suggestions.
Funding
This study was funded by the BONUS + HYPER project and the BONUS COCOA project, which were supported by BONUS (Article 185) funded jointly by the European Union and the National Center for Research and Development (NCBiR, Poland).
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Conceptualization H.K. and U.J.; methodology H.K. and U.J.; formal analysis H.K.; investigation H.K.; data curation H.K.; writing—original draft preparation, H.K. and U.J.; writing—review and editing H.K. and U.J.; visualization, H.K.; project administration, U.J. and H.K.; funding acquisition U.J. and H.K. All authors have read and agreed to the published version of the manuscript.
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Kendzierska, H., Janas, U. Functional diversity of macrozoobenthos under adverse oxygen conditions in the southern Baltic Sea. Sci Rep 14, 8946 (2024). https://doi.org/10.1038/s41598-024-59354-3
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DOI: https://doi.org/10.1038/s41598-024-59354-3
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