Abstract
Seasonal variation in habitat use and animal behavior can alter host contact patterns with potential consequences for pathogen transmission dynamics. The endangered Florida panther (Puma concolor coryi) has experienced significant pathogen-induced mortality and continues to be at risk of future epidemics. Prior research has found increased panther movement in Florida’s dry versus wet seasons, which may affect panther population connectivity and seasonally increase potential pathogen transmission. Our objective was to determine if Florida panthers are more spatially connected in dry seasons relative to wet seasons, and test if identified connectivity differences resulted in divergent predicted epidemic dynamics. We leveraged extensive panther telemetry data to construct seasonal panther home range overlap networks over an 11 year period. We tested for differences in network connectivity, and used observed network characteristics to simulate transmission of a broad range of pathogens through dry and wet season networks. We found that panthers were more spatially connected in dry seasons than wet seasons. Further, these differences resulted in a trend toward larger and longer pathogen outbreaks when epidemics were initiated in the dry season. Our results demonstrate that seasonal variation in behavioral patterns—even among largely solitary species—can have substantial impacts on epidemic dynamics.
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Introduction
Outbreaks of infectious diseases pose significant threats to the population health and conservation of free-ranging wildlife1,2, and seasonality can have profound impacts on the dynamics of these outbreaks3. Langwig et al.4 outlined five mechanisms through which seasonality may alter transmission dynamics via: (1) variation in sociality, (2) birth pulses causing influxes of new susceptibles, (3) variation in habitat use, (4) variation in climatic factors, and (5) variation in host immune function. Yet these are not necessarily mutually exclusive mechanisms. For example, climatic variation itself can drive changes in habitat use5,6 or animal social behaviors7. Given this covariation, an emergent question is whether seasonality contributes to significant changes in behavior and host contact patterns, which then translate to consequent changes in transmission dynamics.
While seasonal changes to host contact structure have been associated with predicted changes in outbreak size and speed of pathogen spread8, the relative impact of seasonality on pathogen dynamics likely varies by factors such as the magnitude of seasonality9, individual pathogen characteristics (e.g., incubation period), and local context. For example, seasonal variation in habitat selection by zebra (Equus quagga) in Namibia has been associated with increased anthrax mortalities in the wet season. However, during a period of severe drought, zebra altered their habitat selection with a consequent reduction in wet season anthrax mortalities6. The relative importance of seasonality on pathogen transmission may be of particular significance for species of conservation concern, for heavily managed populations, or in resource-limited conditions, where such seasonal effects may dictate optimal timing of surveillance, interventions, or other management activities to protect at-risk populations10,11.
One such relevant example is the Florida panther (Puma concolor coryi), an endangered subspecies of puma found only in south Florida. In the early 1990s, only an estimated 20–25 adult panthers remained12. Panther genetic diversity was extremely limited, suggestive of depressive inbreeding12, and appeared to be associated with increased disease burden among panthers13,14. Panthers have been infected with pathogens such as pseudorabies, rabies, notoedric mange, dermatophytosis, and feline leukemia virus (FeLV), which have all resulted in mortality15. Additionally, infectious agents including feline calicivirus, feline immunodeficiency virus, feline panleukopenia virus, and Toxoplasma gondii have all been documented in panthers13,16,17. FeLV spillover from domestic cats, in particular, was responsible for a deadly outbreak among panthers in 2002–200418,19 and is an ongoing threat to the population20,21. While recent research has devoted much effort to discerning drivers of transmission among panthers (e.g.22,23), far less effort has been devoted to understanding how transmission dynamics among panthers may be subject to seasonal variations.
Southern Florida is subject to seasonal climatic variability with a tropical monsoon climate, wherein 75% of total annual precipitation falls on the landscape during the wet season (from May to October24). Ground that is dry one month can shift to non-traversable swampland the next. Such hydrologic changes have been implicated in observed seasonal variation in movement patterns among Florida panthers, with increased movement observed in the dry season25,26. Although contact rates between puma (Puma concolor) in the Greater Yellowstone area have been shown to vary seasonally27, it is unknown if seasonal hydrological changes in the subtropical climate of south Florida and their effect on panther movement or behavior translate to changes in panther population connectivity. Further, it is unclear if seasonal changes in population connectivity would impart measurable, biologically significant impacts on epidemic outcomes.
The objectives of this study were to determine (1) if the panther population is more spatially connected in dry seasons than wet seasons, as measured by home range overlap, and (2) if any differences in connectivity could result in different epidemic outcomes in dry versus wet seasons, and identify transmission conditions under which those difference may be the greatest. We use the term spatial connectivity, hereafter, to refer to the connectedness of individuals within our population as a result of home range or spatial overlap. Results of this study shed light on the impact of social ecology on subsequent pathogen transmission and management in free-ranging wildlife.
Materials and methods
We used very high frequency (VHF) telemetry data previously collected from Florida panthers captured and radiocollared by the Florida Fish and Wildlife Conservation Commission and National Park Service. Captures followed FWC agency guidelines for the immobilization and handling of wild panthers, which have been modeled to closely follow the American Society of Mammalogists’ guidelines for the use of wild mammals in research28. We selected the years of 1996–2007 as our study period, as telemetry coverage during this window showed consistent, high numbers of monitored individuals, with a mean of 36 panthers monitored per year (range 26 to 47; minimum panther population size was approximately 30–90 individuals during this period29). Relocations of radiocollared panthers were recorded from aircraft during the day, typically three times per week. We followed Criffield et al.25 in defining the south Florida wet season as 15 May through 14 October, and dry season as 15 October through 14 May each year (Fig. S1). We reviewed telemetry locations for each individual in each season per year to identify errant or outlier points that were likely erroneous based on extreme divergence from all other points, implying unrealistic speeds of travel; this process resulted in the removal of 13 relocations across the 11 year dataset.
Panther seasonal spatial connectivity
We evaluated seasonal changes in the potential for individuals to interact by estimating spatial overlap between panther home ranges in each year and season30,31,32. We chose spatial overlap as a representation of potential interactions because our data were limited to relocations collected three times per week, which precluded reliably identifying direct contact events. Spatial overlap is representative of the potential for individuals to interact indirectly, and can be representative of direct interactions30, with this approach having been used previously in solitary (Puma concolor33) and social carnivores (wolves, Canis lupus, and lions, Panthera leo34). Further, the long-term nature of our relocation data provided a unique opportunity to examine seasonal patterns across an 11-year period; this is often not feasible with higher temporal resolution GPS collar data due to the shorter battery life of these devices35.
To quantify spatial overlap between panthers, we used the utilization distribution overlap index (UDOI36) with the 95% bivariate normal kernel home range. We chose to use UDOI, as it is the preferred metric when measuring space-use sharing between individuals (as compared to metrics such as volume of intersection and Bhattacharyya’s affinity)36,37. Home range kernels, 95% isopleth areas, and UDOI were estimated using the adehabitatHR package in R (R version 3.6.3 and 4.2.038,39), as the coarseness of our relocation data did not require the use of autocorrelated kernel density estimators40,41.
Weighted networks were then constructed for each season and year, whereby individuals were connected in the network with edges weighted by UDOI (i.e., panthers with very little home range overlap had low edge weights). Our primary analysis did not subset networks by a minimum or threshold UDOI value (hereafter, filter), as such filtering is generally not recommended in network analysis42. However, we also replicated analyses with networks where edges were filtered for UDOI greater than or equal to 0.01 and 0.1 (an additional 44 networks) in order to test the sensitivity of our results to edge definitions.
For each network, we calculated node metrics of degree and strength, and network metrics of density and modularity43, all of which are key indicators of network connectivity and pathogen transmission44. Degree and strength are the number of connections and sum of weighted connections for each node, respectively. Individuals with high degree and/or strength may be candidates as “superspreaders”45. Density is a network-level measure of overall connectivity, while modularity is a measure of network subdivisions which may affect transmission dynamics46. Because networks were different sizes across seasons and years, we calculated the normalized degree for each node (degree divided by n-1, where n is the number of nodes in the network) to facilitate comparisons of networks across the study period. Strength was calculated using UDOI as the edge weight. Density was calculated as the proportion of realized versus possible edges in a network47. Modularity was calculated using a walktrap community-finding algorithm with 4 and 7 steps (to test sensitivity to this choice), with inverse UDOI as the edge weight. All network metrics were calculated using the igraph package in R48.
We tested for differences in the node- and network-level metrics in dry versus wet seasons. Because panthers were often monitored for multiple numbers of seasons and years (i.e., repeated measures within individuals), for node-level metrics (normalized degree and strength), we used a cluster level bootstrap49 to model metric values as a function of log home range area (in square kilometers) and season. We treated individual panthers as clusters (i.e., panthers were the unit that we resampled). Duration of observation (i.e., the number of seasons) was variable across the population, however, resulting in unequal cluster sizes, so our bootstrap approach sampled with replacement based on cluster size (we sampled with replacement individuals who were monitored for two seasons, three seasons, and so on)49. In each bootstrap iteration, we fitted the above linear model and recorded coefficient estimates (bootstrap statistics). We used 1000 iterations per bootstrap analysis, then extracted 95% confidence intervals for each coefficient from the quantiles of the resulting coefficient bootstrap distributions.
In addition, to specifically test if seasonal differences in panther connectivity were linked to hydrological changes (versus an unmeasured metric of seasonality), we compared our node-level metrics to seasonal precipitation. Precipitation data was accessed from the National Oceanic and Atmospheric Administration’s Climate Data Online service50. We averaged daily precipitation totals across Collier County weather stations from May 15, 1996 to May 14, 2007 and summed these averages per season per year to give seasonal total average precipitation values for each year and season. We used Spearman correlation to test for correlations between these total average precipitation values and median node-level metrics per season and year.
For the network-level metrics of density and modularity, we used non-parametric Kruskal–Wallis rank sum tests to test for differences in these metrics in the dry versus wet seasons across the 22 networks (2 seasons for each of 11 years). All Kruskal–Wallis tests were performed using the stats package in R38.
Seasonal network connectivity and epidemic outcomes
We used a simulation approach to determine if differences in network connectivity alter epidemic outcomes in dry versus wet seasons. A simulation approach allowed us to examine transmission of a range of potential pathogens through empirically-informed networks, a strategy that can be helpful for highlighting the conditions or parameter space under which we expect to see the greatest impact of host or pathogen characteristics on epidemic outcomes5,46,51,52,53,54. Furthermore, network size was variable across years and seasons, and the degree distribution of networks can be influenced by network size and density42. Hence, a simulation approach allowed us to limit epistemic uncertainty (variation resulting from experimental uncertainty55) resulting from variation in network size, such that our simulation results would be indicative of differences between wet and dry season networks.
We simulated networks based on normalized degree and UDOI distributions from the observed networks, using separate distributions for dry and wet seasons. For each observed network, we fit a beta distribution to the normalized degree, and a gamma distribution to the observed UDOI edge weights using the fitdistrplus package in R56. We then took the mean of the beta and gamma distribution parameters across the dry and wet seasons, respectively. In addition, we recorded the number of isolates (completely unconnected individuals) in each observed network and, across all dry and all wet season networks, fit separate Poisson distributions to their respective counts of isolates. This approach resulted in three distributions per season describing normalized degree, UDOI, and the number of network isolates.
We then simulated new networks based on these three distributions for each season (degree, UDOI edge weight, and number of isolates). Simulated networks each had 33 nodes, which was the average network size across all observed networks. We simulated networks as single large components (rather than two or more subcomponents), then added isolated individuals by drawing from the corresponding dry or wet season Poisson distribution. Among non-isolates, we simulated a normalized degree distribution from the corresponding dry or wet season beta distribution, and transformed these values to standard degree values based on the population size. We then used simulated annealing in the ergm package in R57 to generate a network with the target degree distribution. Simulated annealing is not always precise, and because we did not detect statistically significant differences in network density between dry and wet seasons (see results), we constrained simulated network density to ± 25% of the average network density across all networks.
To assign edge weights, we followed Reynolds et al.8 and randomly assigned edge weights by drawing from the corresponding gamma distribution for dry versus wet season UDOI values. There was no evidence of correlation between degree and UDOI (treating each UDOI estimate as independent, Kendall’s τ = − 0.06), supporting random assignment of UDOI values in our simulations. Because UDOI values can be greater than one36, we scaled simulated UDOIs by the maximum drawn UDOI per simulated network so all values fell between 0 and 1 for transmission simulations (see Fig. S2 for representative examples of simulated networks).
Network metrics that reflect higher order or indirect network structure (e.g., clustering coefficient, betweenness) are typically less robust to undersampling of populations58,59,60, so we did not simulate networks constrained by these metrics. The impact of undersampling on modularity is less clear60, and we did not specifically constrain the modularity of simulated networks. However, to ensure our simulated epidemic outcomes were robust to higher order network structure (in the form of modularity), we evaluated epidemic outcomes (see below) for the subset of simulations in which simulated network modularity was within the bounds of observed modularity in respective dry and wet seasons.
With each simulated network, we simulated pathogen transmission through that network in weekly time steps. Transmission was initiated with a randomly selected individual and simulations continued for a maximum of six months (representing the length of our seasons). Because our networks were based on spatial overlap between panthers, we expected that our simulations would be most representative of indirectly or environmentally transmitted pathogens (e.g., gastrointestinal helminths, feline panleukopenia virus). However, spatial overlap has been used to represent the potential for direct social interactions between Puma concolor and bobcat (Lynx rufus)33, as well as direct intraspecific associations in other species (e.g.30,31,32). Our simulations may therefore also have relevance to directly transmitted pathogens, assuming a direct relationship between spatial overlap and direct contact. As such, transmission simulations were designed to represent a broad range of potential pathogen types, including those that cause chronic infections (susceptible-infectious model, SI; e.g. directly transmitted feline immunodeficiency virus), those that cause infections that can recur after recovery (susceptible-infectious-susceptible model, SIS; e.g. many indirectly transmitted gastrointestinal helminths), and those that cause infections that induce immunity (susceptible-infectious-recovered model, SIR; e.g. directly or indirectly transmitted feline panleukopenia virus).
Transitions between infection states occurred through a stochastic chain binomial process. In each time step, transmission (transition from susceptible to infectious) occurred for an individual if all the following conditions were met: (1) the existence of an edge between a susceptible and infectious individual; (2) a random binomial draw based on scaled UDOI edge weight (high edge weight = high probability of success); (3) a random binomial draw based on an additional edge weight scaling parameter, ρ, used to adjust the relative effect of UDOI-based edge weights; and (4) a random binomial draw based on the probability of transmission, given contact, represented by β (note that this is different from the conventional β in most compartmental models). UDOI can range from zero to greater than one, and even scaled to range from zero to one, empirical evidence from Puma concolor demonstrates that it is unlikely that even highly overlapping panthers interact on a weekly basis27. We therefore included the parameter ρ to scale UDOI-based contact probabilities to better represent the expected low rate of weekly contact in panthers. Further, inclusion of ρ allowed us to explicitly evaluate epidemic outcomes across a range of probabilities of contact. We repeated all simulations using binary (unweighted) edges instead of UDOI-weighted edges, but still including ρ. In SIS and SIR models, recovery from an infectious state (to susceptible or recovered, respectively) occurred at a rate γ, representing the weekly probability of recovery.
All parameters were varied across a range of parameter space in a full factorial design (Table 1). The combination of three model types (SI, SIS, SIR), weighted versus binary networks, and the potential values for the three transmission parameters (ρ, β, γ) resulted in 210 parameter sets which were instituted across dry and wet season simulation scenarios (for a total of 420 parameter sets). For each parameter set, a full simulation consisted of simulation of either a single dry or wet season network, and a transmission simulation through that network. 100 full simulations were completed for each parameter set in each of dry and wet season scenarios, resulting in a total of 42,000 full simulations.
To compare outcomes of simulated epidemics between dry and wet season scenarios, we recorded several key outcomes from each simulated epidemic. These included: outbreak duration (for SIS and SIR), total number of individuals ever infected, and proportion of outbreaks that failed. Failed outbreaks were defined as those that initiated in an unconnected isolate or in a non-isolate but only affected 1 individual; otherwise, epidemics were considered “successful”. We compared outcomes between wet and dry seasons using heat maps; for outbreak duration and total individuals infected, we compared mean values from successful epidemics. In addition, for the key result of “total ever infected”, we fit a generalized linear mixed model (GLMM), assuming a gamma distribution and log link. In this model, our response variable was the total number of panthers ever infected, with fixed effects for season and simulated network density, and a random intercept for parameter set (which incorporated model type). We controlled for simulated network density in this model to determine if differences in epidemic size were the result of season or simply differences in network density that emerged from simulations. Finally, to examine how our transmission parameters contributed to total infections and outbreak durations, we performed a variable importance analysis using the randomForest package in R61,62. For each of these two outcome variables, we performed a random forest regression with 2000 trees for all simulations infecting more than one individual. For outbreak duration, we only analyzed results from the SIR model type (these outbreaks were less likely to persist through the whole simulation duration).
Results
Panther spatial connectivity was higher in dry seasons than in wet seasons
Florida panther home range overlap networks were generally highly connected, with few observed unconnected individuals across the 22 seasonal networks (Fig. 1, Figs. S3–S13). Overlap, as quantified by UDOI, was right-skewed such that most pairs had low overlap and a smaller proportion had high degrees of overlap (Table S1, Fig. S14). Node-level metrics of normalized degree and strength were higher in dry seasons than in wet seasons (Fig. 2, Table 2). Because we used normalized degree as our response variable in the former model, effect sizes represent changes in node degree per n-1 individuals in a network. As such, seemingly small effect sizes can be associated with larger impacts in realized networks. Larger home range (HR) sizes contributed to higher node level metrics, but did not wholly account for the observed seasonal differences (Table 2, Figs. S15, S16). Furthermore, dry seasons had higher node-level metrics than wet seasons even when filtering edges by higher UDOI values (Table S2). Median values for both node-level metrics were positively correlated with seasonal total average precipitation in dry seasons, while wet seasons were negatively correlated, though these correlations did not achieve statistical significance (note small sample sizes; Fig. 3, Table S3).
In contrast, network-level metrics showed little to no differences between wet and dry seasons, and this was largely consistent across levels of UDOI edge filtering (Table S4). Modularity was higher in dry seasons than wet seasons (median modularity with 4 steps: dry = 0.62, wet = 0.47; with 7 steps: dry = 0.64, wet = 0.47), but only without UDOI filtering for edges, and this difference did not reach statistical significance (with no edge filtering, rank sum = 3.75; p = 0.053; Table S4). Network density was not statistically significantly different between wet and dry seasons at any level of UDOI filtering (with no edge filtering, rank sum = 1.99; p = 0.16; Table S4).
Greater dry season connectivity led to more infected individuals
Simulated epidemic outcomes demonstrated a trend toward more infected individuals, more successful epidemics, and longer durations of epidemics when the onset was in the dry season versus the wet season (Fig. 4, Figs. S17–S23). These findings were largely consistent across model types and parameter space. The maximum difference in mean proportion of individuals infected in the dry season relative to the wet season for SI, SIS, and SIR models was 12.5%, 17.8%, and 16.5%, respectively (Fig. 4, Figs. S17, S18). More generally, the greatest differences in number of individuals infected per season were typically apparent with intermediate to high transmission potential (combination of probability of transmission and edge weight scaling), and intermediate to long duration of infection (intermediate to low weekly probability of recovery; Fig. 4, Figs. S17, S18). When repeating simulations with networks with binary edges (no UDOI weighting), epidemics were typically much larger (often affecting all individuals) than in simulations with weighted edges, yet still showed a consistent trend for larger epidemics in the dry season than the wet season (Figs. S24–S26).
Examination of epidemic curves demonstrated that the differences in epidemic dynamics were more pronounced at some time points (Fig. 5, Fig. S27). For the SIR model producing the largest differences between dry and wet season outbreak sizes (probability of transmission given contact = 0.6, edge weight modifier = 1, weekly probability of recovery = 0.25), dry season epidemics had up to about two more infectious individuals at one time, compared to the wet season (Fig. S27). Because simulated population sizes were only 33 individuals, this reflects about 6% more of the population being infected at a single time point.
While observed networks did not show differences in density between dry and wet seasons, simulated network density, which was an emergent property of simulations, was higher in simulated dry season networks than wet season networks (Fig. S28). This was true despite having constrained densities to the same range of values for wet and dry seasons in our network simulation process, and therefore likely emerged from the differing underlying degree distributions. When accounting for simulated network density in our GLMM, season did not predict the total number of individuals infected in simulations (season: estimate = 0.01, standard error = 0.02, p = 0.7; network density: estimate = 5.4, standard error = 0.38, p < 0.001).
When evaluating the subset of simulation results in which simulated network modularity was within the ranges from observed networks, differences in epidemic sizes between dry and wet seasons were reduced but not eliminated (Figs. S29–S31). Again, this finding was true across model types and parameter space.
Our variable importance analysis found that the edge weight modifying and transmission probability parameters (ρ and β) were the most important for total simulated infections, and, along with the recovery probability parameter (γ), had a nonlinear relationship with this simulated outcome (Figs. S32, S33). In contrast, the recovery probability parameter (γ) was most important for the duration of SIR outbreaks, with a more linear relationship (Figs. S34, S35).
Discussion
In this study, we first tested for differences in seasonal spatial connectivity in an endangered carnivore, the Florida panther. Our network approach indicated more and stronger spatial overlap among panthers during Florida’s dry season. We then determined if the observed differences in connectivity resulted in changes to predicted epidemic dynamics, finding that dry season outbreaks were consistently larger, of longer duration, and more likely to be successful.
We found that panther spatial overlap networks were more highly connected in dry seasons than wet seasons. This seasonality in overlap means that panthers likely have increased indirect contact in the dry season, and may also have increased direct contact during this period, assuming that direct contact is directly related to spatial overlap. Our results were consistent even when filtering network edges by higher home range overlap values (UDOI) and accounting for panther home range size, suggesting that the increased connectivity in dry seasons is not simply the result of weak home range overlap connections. Rather, seasonal behavioral changes or alterations in specific habitat use or movement likely contribute to increased spatial connectivity in panthers.
Panthers will reproduce throughout the year, but the majority of litters are born in February–June; with a 90 day gestation, this places most mating interactions in the dry season64. Furthermore, the dry season coincides with peak parturition of key panther prey species (i.e., white-tailed deer, Odocoileus virginianus, and feral swine, Sus scrofa64), which could alter panther habitat use as female panthers prioritize these young prey resources. Previous work has also implicated hydrological changes during the wet season with changes in panther movement; specifically decreased daily movements and shorter step lengths25. Decreased “ease of movement” during the wet season would explain the decreased spatial overlap connectivity we observed. In support of this hypothesis, we observed negative correlations between median values of node-level metrics of connectivity (degree and strength) and wet season precipitation (as total average precipitation), though this relationship did not achieve statistical significance. However, contrary to our expectations, we also observed positive correlations between node-level metrics and dry season precipitation, suggesting that spatial connectivity is the lowest in the driest dry seasons and the wettest wet seasons. This seemingly non-monotonic relationship between precipitation and spatial connectivity could be explained by increasing availability of usable habitat (e.g., unflooded) in the driest dry seasons, thereby reducing competition for spatially structured resources and consequent spatial overlap. In contrast, the wettest wet seasons may then favor increasing spatial isolation of individuals. Alternatively, if prey species become congregated in small areas during wet seasons, panthers may aggregate in these areas, facilitating increased competition and potential aggressive interactions. Aggregations of prey can also facilitate trophic transmission of pathogens like pseudorabies virus, where higher local prey densities increase intra-species transmission, thereby increasing the probability of spillover to predators65. The nature of interactions between panthers in areas of spatial overlap therefore merits investigation, particularly for the study of directly transmitted pathogens.
The mechanisms underlying the seasonal changes in spatial connectivity we observed here are likely nonlinear and multifactorial. Future work with higher resolution movement data could refine our understanding of the relationship between hydrology and panther space use, as well as examine if the changes we observed in panther spatial connectivity are due to increases in specific interactions (e.g., mating) or increased overlap in key habitat areas such as movement corridors or high-quality hunting areas. In panthers and other species of conservation concern, these key overlap areas may be particular regions to target for landscape preservation66 to conserve population genetic connectivity and key resources (e.g. hunting sites67). Alternatively, these could be locations supporting specific behaviors that can facilitate pathogen transmission68,69. For example, in territorial species, scent marking sites may be attractive during mating seasons but otherwise avoided, yielding seasonal differences in indirect contact and potential variation in pathogen outbreak dynamics62,70. Similarly, sharing kill sites of large prey may facilitate panther interactions (as in Puma concolor71) and consequent disease transmission as availability of larger prey varies over space or time. In addition, climate change is likely to compound or alter seasonal variation in spatial connectivity in panthers and other wildlife species. For example, sea level rise and precipitation changes as a result of climate change are expected to significantly alter Florida’s seasonal hydrological dynamics72. As such, future work should consider how such climate-induced landscape changes may affect resource availability, habitat quality and fragmentation, and how these changes may interact with other stressors to wildlife (e.g., human encroachment or alterations in prey availability) or additional climate change effects, such as increased survival of environmental pathogens2.
As a result of seasonal changes in panther spatial connectivity, simulated epidemics were consistently larger among panthers when an outbreak was initiated in the early dry season. This was particularly true for parameter space which may represent moderate to highly transmissible pathogens such as feline panleukopenia virus or feline herpesvirus73. Our variable importance analysis identified a potentially nonlinear relationship between outbreak size and the parameters representing pathogen transmissibility and scaled contact rates (β and ρ, respectively), with rapid increases in outbreak size observed at intermediate values of these parameters. This nonlinearity may contribute to a thresholding effect, above which outbreaks are more substantial and likely to produce biologically significant differences between seasons. While we lacked seasonal epidemiological data for specific pathogens in panthers, our results are consistent with observed seasonal differences in pathogen prevalence in other species, including cases that appear to be driven by seasonality in host contact patterns, behavior, or movement (e.g., rabies in several skunk species74; Mycoplasma gallisepticum in house finches, Haemorhous mexicanus75,76; phocine distemper virus in harbor seals, Phoca vitulina77,78). However, in the case of Tasmanian devils (Sarcophilus harrisii), observed seasonality in contact and biting patterns79,80,81 has not been associated with seasonal variation in new devil facial tumor disease cases82. This discrepancy may be the result of heterogeneity in the latent period before tumors become apparent54, and highlights that our results for simulated panther outbreaks could be altered by individual-level heterogeneities that we did not include here.
While observed dry season networks tended to be more modular—which might be expected to reduce outbreak size44—even when constraining simulation results to match this pattern, dry season outbreaks still resulted in more infected individuals than wet season outbreaks. This supports the conclusion that observed modularity was inadequate to overcome the increased transmission resulting from increased connectivity in dry seasons. Alternatively, our simulated networks were predominantly single large components, rather than multiple smaller subcomponents, the latter of which was observed in our empirical panther networks and might be expected to limit the extent of outbreaks. Panthers located in the Everglades at the far southeastern extent of panther habitat can be somewhat isolated from the broader population, but pathogens from the broader population can still reach these individuals22. Thus, our simulated, single component networks are still representative of broader population connectivity patterns, and reinforce the findings of Sah et al.46 that the ability of modularity to mitigate epidemics appears to be strongest with extremely high subdivision of networks.
The differences in predicted epidemic sizes between dry and wet seasons were typically minor but substantial in some cases, particularly for parameter space that generally produced intermediate to large epidemics (i.e., again most representative of pathogens such as feline panleukopenia virus or feline herpesvirus). When managing small populations, especially those such as panthers with limited host genetic diversity12, seemingly minor epidemic changes may pose significant risk to population health. For example, a cumulative increase in average infections approaching 18%—which we observed here—may represent substantial risk to population viability, where single individuals may be important for population genetic diversity83. Given that climate and land-use change are expected to increase novel inter-species viral transmission events84, we can expect that species of conservation concern like panthers will face new and emerging pathogen threats into the future. Our results help highlight seasonal vulnerability of the panther population and can direct management actions moving forward. For example, Florida panther capture and management activities predominantly occur during Florida’s dry season, and our results indicate this timing should be helpful for detecting emerging outbreaks when they could be more dangerous to population-level panther health.
The differences in epidemic outcomes that we observed here are likely mitigated by the low contact rates exhibited by Puma concolor more generally27. In contrast to solitary panthers, more gregarious or fission–fusion species would be expected to demonstrate larger differences in epidemic dynamics between seasons in the context of seasonal differences in connectivity. For example, seasonal contact differences in pastoral cattle herds in eastern Africa were found to result in significant changes in simulated epidemic dynamics5. The risks of sociality, however, may be mitigated by heterogeneity in individual associations53. Together, these results demonstrate the importance of linking movement, social, and disease ecology in order to understand the impact of seasonal space use and behavior changes on pathogen transmission.
An important observation in this study was finding that node-level metrics of connectivity were increased in dry seasons, while the network-level metric of connectivity, density, did not appear to vary between seasons. Prior research has shown that degree and strength are generally less sensitive to sampling limitations than density60. This suggests that our observed node-level metrics were more representative of connectivity differences than the observed densities. Interestingly, simulated network densities, emergent properties of our network simulations, did show differences between wet and dry seasons, and were an important predictor in simulated outbreak sizes. Degree distribution and density are closely linked43, suggesting that our empirical degree distributions should have been associated with more significant differences in network density than were captured by the sampling approach used here. As such, the predicted differences in outbreak dynamics by season likely represent a real but empirically unobserved effect of network connectivity, especially given the negligible effects of network modularity we observed. This finding highlights the importance of considering a suite of network attributes when assessing network connectivity and the effect of connectivity on outbreak dynamics.
This study made use of an extensive database of Florida panther relocations, but the frequency of relocations was limited to three weekly (which is a remarkable achievement for aerially relocated VHF collars over more than three decades). This limited our analysis of panther connectivity to an assessment of home range overlap. Home range overlap can sometimes be representative of direct contact and contact rates in free-ranging wildlife (e.g.30), and has been used previously to describe network structure in Puma concolor33. While research suggests that the amount of home range overlap may not correlate well with the number of direct contacts in territorial species, the spatial constraints imposed by hard habitat boundaries on the Florida panther population (along with a growing panther population29,85) result in a more aggregated population that is more amenable to extrapolating spatial overlap to direct contact network structures60. We therefore consider our simulations most relevant to indirectly transmitted pathogens, as well as potentially applicable to directly transmitted agents. Additional work comparing spatial overlap to direct contact rates in panthers and other wildlife species would help refine our understanding of when spatial overlap is most relevant as a proxy for direct contact rates. Thus, while we did not simulate specific pathogens and were limited to spatial overlap as an indicator of population connectivity, our results are able to set expectations for relative epidemic size between seasons across a range of pathogen types.
A significant uncertainty in our simulations was the expected weekly contact rate between panthers. We addressed this uncertainty by exploring epidemic dynamics across a range of possible contact rates, as represented by our edge weight scaling parameter; however, poorly characterized contact rates are an ongoing challenge in studies of panthers and other elusive wildlife species35. Further research with higher resolution movement data, especially in conjunction with proximity loggers, would be ideal to characterize contact rates and behaviors for future behavioral studies and pathogen transmission models. In addition, while we incorporated individual-level heterogeneity in contact patterns through our network approach, additional layers of heterogeneity are likely to affect seasonal variability in epidemic outcomes. For example, host immune response (i.e., susceptibility) or pathogen shedding may vary between individuals86 and seasons3, adding to the complexity of seasonal outbreak dynamics. Furthermore, while we assumed transmission within a single host species, multi-host pathogens are also affected by seasonality, including temporal variation in contact patterns between host species (e.g.87). As such, future work would benefit from examining how additional host and pathogen heterogeneities interact with seasonal dynamics to alter transmission processes.
Conclusions
Florida panther spatial connectivity increased during south Florida’s dry season; these changes can result in substantial differences in epidemic dynamics, though these effects are likely mitigated by the low contact rates exhibited by this solitary carnivore. Regardless, ongoing conservation and management of panthers should benefit from a precedent of performing most capture and handling activities in the dry season, which may facilitate detection of outbreaks when they would have the greatest negative impact. This work demonstrates the importance of linking movement, social, and disease ecology to understand temporally dynamic risks to population health.
Data availability
Full R code for all analyses and simulations is available on GitHub (https://github.com/mjones029/Seasonal_panther_networks) and archived at Zenodo (https://doi.org/10.5281/zenodo.10011452).
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Acknowledgements
This research was supported by the National Science Foundation (DEB-1413925, 1654609, and 2030509). MLJG was supported by the Office of the Director, National Institutes of Health (NIH T32OD010993), the University of Minnesota Informatics Institute MnDRIVE program, and the Van Sloun Foundation. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Florida panther data collected by the Florida Fish and Wildlife Conservation Commission is fully supported by donations to the Florida Panther Research and Management Trust Fund via the registration of “Protect the Panther” license plates. The authors acknowledge the efforts of National Park Service staff in the collection of Florida panther data utilized in this study.
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M.L.J.G., S.N.H., K.V., D.O., M.C., S.V., and M.E.C. designed the study. M.L.J.G. and S.N.H. performed analyses. M.L.J.G. led the writing of the manuscript. All authors provided critical review of the manuscript and approved submission.
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Gilbertson, M.L.J., Hart, S.N., VanderWaal, K. et al. Seasonal changes in network connectivity and consequences for pathogen transmission in a solitary carnivore. Sci Rep 13, 17802 (2023). https://doi.org/10.1038/s41598-023-44815-y
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DOI: https://doi.org/10.1038/s41598-023-44815-y
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